Detoxification of Lignocellulosic Hydrolysates for Improved Bioethanol Production

Chandel, Anuj K.; Silva, Sílvio Silvério da; Singh, Om V.

doi:10.5772/16454

Cited by 23 publications

(9 citation statements)

References 94 publications

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“…24 To improve the 2,3-BDO production from hydrolysates, treatment processes to remove microbial inhibitors prior to fermentation are still required. 25 Therefore, a microwave-assisted heating-activated carbon adsorption method was employed to detoxify furfural and phenol from cotton stalk hydrolysate under the following conditions: activated carbon of 1%, microwave power of 330 W and detoxification time of 10 min. 26 This processing removed 81.2% of furfural and 92.3% of phenol but only 10.6% of the total sugar loss.…”

Section: Optimization Of the Cotton Stalk Hydrolysate Concentrationmentioning

confidence: 99%

Adaptive laboratory evolution of Klebsiella pneumoniae for improving 2,3-butanediol production

Li¹,

Zhang²,

Dang

2016

Bioengineered

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Microbial production of 2,3-butanediol is limited by the toxic components in the lignocellulose hydrolysate. To improve the 2,3-butanediol production via Klebsiella pneumoniae from cotton stalk hydrolysate, a method coupling a high tolerance of strain and detoxification of the hydrolysate was thus investigated in this study. The strain tolerance of K. pneumoniae to the cotton stalk hydrolysate was improved via an adaptive laboratory evolution, which involved a stepwise increase in the hydrolysate concentration in the medium. Compared with the initial strain, the resulting strain increased the biomass 3.2-fold in a medium of 20 g/L hydrolysate and produced 10.45 g/L of 2,3-butanediol at an optimal concentration of 60 g/L hydrolysate. After detoxification of cotton stalk hydrolysate, the cell metabolism of K. pneumoniae was further promoted, and the 2,3-butanediol production increased by 1.2 folds. Using fed-batch fermentation, the concentration of 2,3-butanediol reached 35.5 g/L with a yield of 0.43 g/g. The results demonstrated that the bioconversion of low-cost cotton stalk hydrolysate into 2,3-butanediol improves the economics of microbial 2,3-butanediol production.

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Section: Optimization Of the Cotton Stalk Hydrolysate Concentrationmentioning

confidence: 99%

Adaptive laboratory evolution of Klebsiella pneumoniae for improving 2,3-butanediol production

Li¹,

Zhang²,

Dang

2016

Bioengineered

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“…Usually less toxic substances are formed in catalytic processes like hydrolysis than in thermochemical depolymerization processes such as pyrolysis, but nevertheless this may lead to issues when applied in biological systems (Arnold, Moss, Henkel, & Hausmann, 2017;Jönsson, Alriksson, & Nilvebrant, 2013;Palmqvist & Hahn-Hägerdal, 2000). The inhibitor concentrations showed great fluctuation between different hydrolyzates, as summarized by (Chandel, da Silva, & Singh, 2011). Acetic acid concentration varies from 0.4 g/L (Alriksson, Cavka, & Jönsson, 2011) to 5.45 g/L , furfural concentration from 0.15 g/L (Nigam, 2001) to 2.2 g/L (Qian et al, 2006), and hydroxymethylfurfural (HMF) concentration from 0.07 g/L (Villarreal, Prata, Felipe, & Almeida E Silva, 2006) to 3.3 g/L (Alriksson et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Potential of biotechnological conversion of lignocellulose hydrolyzates by Pseudomonas putida KT2440 as a model organism for a bio‐based economy

et al. 2019

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Lignocellulose‐derived hydrolyzates typically display a high degree of variation depending on applied biomass source material as well as process conditions. Consequently, this typically results in variable composition such as different sugar concentrations as well as degree and the presence of inhibitors formed during hydrolysis. These key obstacles commonly limit its efficient use as a carbon source for biotechnological conversion. The gram‐negative soil bacterium Pseudomonas putida KT2440 is a promising candidate for a future lignocellulose‐based biotechnology process due to its robustness and versatile metabolism. Recently, P. putida KT2440_xylAB which was able to metabolize the hemicellulose (HC) sugars, xylose and arabinose, was developed and characterized. Building on this, the intent of the study was to evaluate different lignocellulose hydrolyzates as platform substrates for P. putida KT2440 as a model organism for a bio‐based economy. Firstly, hydrolyzates of different origins were evaluated as potential carbon sources by cultivation experiments and determination of cell growth and sugar consumption. Secondly, the content of major toxic substances in cellulose and HC hydrolyzates was determined and their inhibitory effect on bacterial growth was characterized. Thirdly, fed‐batch bioreactor cultivations with hydrolyzate as the carbon source were characterized and a diauxic‐like growth behavior with regard to different sugars was revealed. In this context, a feeding strategy to overcome the diauxic‐like growth behavior preventing accumulation of sugars is proposed and presented. Results obtained in this study represent a first step and proof‐of‐concept toward establishing lignocellulose hydrolyzates as platform substrates for a bio‐based economy.

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“…Different purification processes to remove contaminants have been described by Chandel et al 21 These methods include chemical/physical conditioning steps 22 followed by evaporative concentration methods. 23 The conditioning steps generate large amounts of solid waste whose disposal can be expensive and pose environmental concerns.…”

Section: Introductionmentioning

confidence: 99%

From Biomass to Sugar Alcohols: Purification of Wheat Bran Hydrolysates Using Boronic Acid Carriers Followed by Hydrogenation of Sugars over Ru/H-ZSM-5

Sanchez‐Bastardo

Delidovich

Alonso

2018

ACS Sustainable Chem. Eng.

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Wheat bran is a lignocellulosic waste of milling industry. It contains hemicelluloses which can be valorized into arabitol and xylitol via a few-step approach. It begins with extraction and hydrolysis of hemicelluloses to produce a solution of xylose and arabinose along with proteins and inorganic salts. This work focusses on the purification of sugars of this hydrolysate and the subsequent catalytic production of sugar alcohols. A purification process based on the recovery of sugars by anionic extraction with a boronic acid, followed by back-extraction and a further refining step with ion exchange resins is described. After this process, a high purity sugars solution (~90%) free of inorganic elements and proteins was obtained. The feasibility of the process was also highlighted by a successful recycling of the organic phase containing the boronic acid. The hydrogenation of purified sugars was then performed over Ru/H-ZSM-5. A high yield into pentitols of ~70% with 100% selectivity was achieved. Importantly, the catalytic hydrogenation of sugars in the hydrolysate prior to purification did not occur. We determined that proteins caused the deactivation of the catalyst and consequently the inhibition of the production of sugar alcohols.

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Detoxification of Lignocellulosic Hydrolysates for Improved Bioethanol Production

Cited by 23 publications

References 94 publications

Adaptive laboratory evolution of Klebsiella pneumoniae for improving 2,3-butanediol production

Adaptive laboratory evolution of Klebsiella pneumoniae for improving 2,3-butanediol production

Potential of biotechnological conversion of lignocellulose hydrolyzates by Pseudomonas putida KT2440 as a model organism for a bio‐based economy

From Biomass to Sugar Alcohols: Purification of Wheat Bran Hydrolysates Using Boronic Acid Carriers Followed by Hydrogenation of Sugars over Ru/H-ZSM-5

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