Detoxification of mycotoxin patulin by the yeast Rhodosporidium paludigenum

Zhu, Ruiyu; Feussner, Kirstin; Wu, Tao; Yan, Fujie; Karlovský, Petr; Zheng, Xiaodong

doi:10.1016/j.foodchem.2015.01.066

Cited by 120 publications

(87 citation statements)

References 28 publications

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“…Fifty-one percent of patulin was absorbed by dead yeast cells of R. paludigenum [28]. However, the test on the role of P. caribbica in patulin absorption indicated that patulin was not detected in the broken yeast cells and the cell wall.…”

Section: Discussionmentioning

confidence: 99%

“…R. paludigenum was observed to have degraded patulin through the activities of its intracellular enzymes, and the enzymes were induced by the patulin [28]. In this way, the intracellular enzymes of P. caribbica alone and the P. caribbica induced with patulin were extracted and their ability to degrade patulin was confirmed.…”

Section: Discussionmentioning

confidence: 99%

“…A study by Coelho, Celli, Ono, Wosiacki, Hoffmann, Pagnocca, and Hirooka [26] showed that antagonistic yeast cells incubated with PAT decreased its contamination through two mechanisms: (1) PAT adsorption at the yeast cell wall, and (2) PAT absorption into yeast cells. Zhu et al [28] pointed out that, when dead and live cells of Rhodosporidium paludigenum were incubated with patulin for three days, 51% of the patulin was absorbed by the dead cells while no patulin was detected in the viable yeast cells.…”

Section: Introductionmentioning

confidence: 99%

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The Possible Mechanisms Involved in Degradation of Patulin by Pichia caribbica

Zheng¹,

Yang²,

Zhang³

et al. 2016

Toxins

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In this work, we examined the mechanisms involved in the degradation of patulin by Pichia caribbica. Our results indicate that cell-free filtrate of P. caribbica reduced patutlin content. The heat-killed cells could not degrade patulin. However, the live cells significantly reduced the concentration of the patulin. In furtherance to this, it was observed that patulin was not detected in the broken yeast cells and cell wall. The addition of cycloheximide to the P. caribbica cells decreased the capacity of degradation of patulin. Proteomics analyses revealed that patulin treatment resulted in an upregulated protein which was involved in metabolism and stress response processes. Our results suggested that the mechanism of degradation of patulin by P. caribbica was not absorption; the presence of patulin can induce P. caribbica to produce associated intracellular and extracellular enzymes, both of which have the ability to degrade patulin. The result provides a new possible method that used the enzymes produced by yeast to detoxify patulin in food and feed.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Possible Mechanisms Involved in Degradation of Patulin by Pichia caribbica

Zheng¹,

Yang²,

Zhang³

et al. 2016

Toxins

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show abstract

“…In some cases, however, the biotransformation product is not detectable through chemical analysis methods, and indirect methods must therefore be adopted to distinguish between adsorption and biotransformation. The involvement of an enzymatic reaction, rather than simple binding, is evidenced by the loss of biotransformation activity following heat or acid-inactivation of cells or in an extracellular extract treated with enzyme inactivating agents, such as proteinase K, SDS or EDTA [12,15,19,98,99]. Furthermore, the subcellular location of active components responsible for biotransformation can be identified by comparing activities in the whole cell culture, extracellular extract (by centrifugation) and cytoplasmic extract (by cell lysis) [25,27,34,45,50,59,88,99].…”

Section: Strategies and Methodologiesmentioning

confidence: 99%

Strategies and Methodologies for Developing Microbial Detoxification Systems to Mitigate Mycotoxins

Zhu

Hassan

Lepp

et al. 2017

Toxins

100

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Mycotoxins, the secondary metabolites of mycotoxigenic fungi, have been found in almost all agricultural commodities worldwide, causing enormous economic losses in livestock production and severe human health problems. Compared to traditional physical adsorption and chemical reactions, interest in biological detoxification methods that are environmentally sound, safe and highly efficient has seen a significant increase in recent years. However, researchers in this field have been facing tremendous unexpected challenges and are eager to find solutions. This review summarizes and assesses the research strategies and methodologies in each phase of the development of microbiological solutions for mycotoxin mitigation. These include screening of functional microbial consortia from natural samples, isolation and identification of single colonies with biotransformation activity, investigation of the physiological characteristics of isolated strains, identification and assessment of the toxicities of biotransformation products, purification of functional enzymes and the application of mycotoxin decontamination to feed/food production. A full understanding and appropriate application of this tool box should be helpful towards the development of novel microbiological solutions on mycotoxin detoxification.

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“…Patulin (PAT), depicted in figure 6, is most commonly and significantly detected from Penicillium expansum contamination of apples and apple products, though many species belonging to the Penicillium, Aspergillus or Byssochlamys fungal genera are acknowledged producers, with other fruits, vegetables, and cereals also found as substrates (Zhu et al 2015;Zouaoui et al 2015).…”

Section: Patulinmentioning

confidence: 99%

Dietary mycotoxins, co-exposure, and carcinogenesis in humans: Short review

Ruyck

Boevre

Huybrechts

et al. 2015

Mutation Research/Reviews in Mutation Research

230

148

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Mycotoxins, toxic secondary metabolites of fungi, affect global agriculture so prolifically that they are virtually ubiquitous at some concentration in the average human diet. Studies of in vitro and in vivo toxicity are discussed, leading to investigations of co-exposed mycotoxins, as well as carcinogenic effects. Some of the most common and toxicologically significant mycotoxins, such as the aflatoxins, ochratoxins, fumonisins, deoxynivalenol, T-2 toxin, HT-2 toxin, patulin, zearalenone, and some ergot alkaloids are outlined. The wide variety of pathogenic mechanisms these compounds employ are shown capable of inducing a complex set of interactions. Of particular note are potential synergisms between mycotoxins with regard to carcinogenic attributable risk, indicating an important field for future study.

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Detoxification of mycotoxin patulin by the yeast Rhodosporidium paludigenum

Cited by 120 publications

References 28 publications

The Possible Mechanisms Involved in Degradation of Patulin by Pichia caribbica

The Possible Mechanisms Involved in Degradation of Patulin by Pichia caribbica

Strategies and Methodologies for Developing Microbial Detoxification Systems to Mitigate Mycotoxins

Dietary mycotoxins, co-exposure, and carcinogenesis in humans: Short review

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