Detoxification of ochratoxin A by Lysobacter sp. CW239 and characteristics of a novel degrading gene carboxypeptidase cp4

Wei, Wei; Yan, Qiushi; Wu, Yanbo; Chen, Ying; Peng, Chengbin; Luo, Mingzhong; Zhou, Yu

doi:10.1016/j.envpol.2019.113677

Cited by 45 publications

(37 citation statements)

References 42 publications

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“…De Bellis et al [ 44 ] and Wei et al [ 45 ] identified the biodegradation products of OTA of some strains, although they did not explore whether OTA participated in metabolic degradation. The biodegradation of OTA by the FS-UV-21 strain has been determined, but the specific metabolites of OTA and the mechanism of metabolic degradation are still unknown.…”

Section: Resultsmentioning

confidence: 99%

Degradation of Ochratoxin A by a UV-Mutated Aspergillus niger Strain

Dong

et al. 2022

Toxins

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Ochratoxin A (OTA) is a mycotoxin that can contaminate a wide range of crops such as grains and grapes. In this study, a novel fungal mutant strain (FS-UV-21) with a high OTA degradation rate (74.5%) was obtained from Aspergillus niger irradiated with ultraviolet light (15 W for 20 min). The effect of pH, temperature, and inoculation concentration on the degradation of OTA by FS-UV-21 was investigated, and the results revealed that the detoxification effect was optimal (89.4%) at a pH of 8 and a temperature of 30 °C. Ultra-performance liquid chromatography-tandem mass spectrometry was used to characterize the degraded products of OTA, and the main degraded product was ochratoxin α. Triple quadrupole-linear ion trap-mass spectrometry combined with LightSight software was used to analyze the biotransformation pathway of OTA in FS-UV-21, to trace the degraded products, and to identify the main metabolite, P1 (C19H18ClNO6, m/z 404). After the FS-UV-21 strain was treated with OTA, the HepG2 cellular toxicity of the degradation products was significantly reduced. For the real sample, FS-UV-21 was used to remove OTA from wheat bran contaminated by mycotoxins through fermentation, resulting in the degradation of 59.8% of OTA in wheat bran. Therefore, FS-UV-21 can be applied to the degradation of OTA in agricultural products and food.

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Section: Resultsmentioning

confidence: 99%

Degradation of Ochratoxin A by a UV-Mutated Aspergillus niger Strain

Dong

et al. 2022

Toxins

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“…Likewise, the D-Ala-D-Ala carboxypeptidase originated from B. amyloliquefaciens ASAG1 could degrade 41% of OTA at 28 °C after 12 h of incubation [ 33 ], while the carboxypeptidase cp4 derived from Lysobacter sp. CW239 could degrade 36.8% of OTA after 24 h of incubation [ 35 ]. The Michaelis constant ( K m ) can reflect the affinity of an enzyme for its substrate, while V max represents the rate of an enzyme-catalyzed reaction when the substrate concentration reaches saturation [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

“…Likewise, a series of carboxypeptidases derived from different strains, including B. amyloliquefaciens ASAG1 [ 33 ], Lysobacter sp. CW239 [ 35 ], and Acinetobacter sp. neg1 [ 39 ] could also degrade OTA into OTα.…”

Section: Discussionmentioning

confidence: 99%

“…Researchers have studied carboxypeptidases from Bacillus amyloliquefaciens ASAG1 [ 33 ], Bacillus subtilis CW14 [ 34 ], and Lysobacter sp. CW239 [ 35 ]. Although a number of articles have reported different kinds of carboxypeptidases derived from different OTA-degrading strains, the mechanism and purified enzyme of a novel screened strain Bacillus subtilis have yet to be researched.…”

Section: Introductionmentioning

confidence: 99%

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Bacillus subtilis ANSB168 Producing d-alanyl-d-alanine Carboxypeptidase Could Alleviate the Immune Injury and Inflammation Induced by Ochratoxin A

Qing

Huo

Huang

et al. 2021

IJMS

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Ochratoxin A (OTA) is toxic to animals and threatens food safety through residues in animal tissues. A novel degrading strain Bacillus subtilis ANSB168 was isolated and further investigated. We cloned d-alanyl-d-alanine carboxypeptidase DacA and DacB from ANSB168 and over-expressed them in Escherichia coli Rosetta (DE3). Then, we characterized the OTA degradation mechanism of DacA and DacB, which was degrading OTA into OTα. A total of 45 laying hens were divided into three equal groups. The control group was fed basal feed, and other groups were administered with OTA (250 μg/kg of feed). A freeze-dried culture powder of ANSB168 (3 × 107 CFU/g, 2 kg/T of feed) was added to one of the OTA-fed groups for 28 days from day one of the experiment. We found that OTA significantly damaged the kidney and liver, inducing inflammation and activating the humoral immune system, causing oxidative stress in the layers. The ANSB168 bioproduct was able to alleviate OTA-induced kidney and liver damage, relieving OTA-induced inflammation and oxidative stress. Overall, DacA and DacB derived from ANSB168 degraded OTA into OTα, while the ANSB168 bioproduct was able to alleviate damages induced by OTA in laying hens.

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“…Carboxypeptidases produced by Bacillus amyloliquefaciens ASAG1 and Lysobacter sp. CW239 were shown to biodegrade OTA [92,93]. Trichosporon mycotoxinivorans also cleaves OTA into phenylalanine and OTα in vitro and its inclusion in their diet blocked the detrimental effects of OTA on several variables of the immune system in broilers [94].…”

Section: Microbial Degradation Of Mycotoxin and Feed Additivesmentioning

confidence: 99%

Mycotoxin and Gut Microbiota Interactions

Guerre

2020

Toxins

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The interactions between mycotoxins and gut microbiota were discovered early in animals and explained part of the differences in susceptibility to mycotoxins among species. Isolation of microbes present in the gut responsible for biotransformation of mycotoxins into less toxic metabolites and for binding mycotoxins led to the development of probiotics, enzymes, and cell extracts that are used to prevent mycotoxin toxicity in animals. More recently, bioactivation of mycotoxins into toxic compounds, notably through the hydrolysis of masked mycotoxins, revealed that the health benefits of the effect of the gut microbiota on mycotoxins can vary strongly depending on the mycotoxin and the microbe concerned. Interactions between mycotoxins and gut microbiota can also be observed through the effect of mycotoxins on the gut microbiota. Changes of gut microbiota secondary to mycotoxin exposure may be the consequence of the antimicrobial properties of mycotoxins or the toxic effect of mycotoxins on epithelial and immune cells in the gut, and liberation of antimicrobial peptides by these cells. Whatever the mechanism involved, exposure to mycotoxins leads to changes in the gut microbiota composition at the phylum, genus, and species level. These changes can lead to disruption of the gut barrier function and bacterial translocation. Changes in the gut microbiota composition can also modulate the toxicity of toxic compounds, such as bacterial toxins and of mycotoxins themselves. A last consequence for health of the change in the gut microbiota secondary to exposure to mycotoxins is suspected through variations observed in the amount and composition of the volatile fatty acids and sphingolipids that are normally present in the digesta, and that can contribute to the occurrence of chronic diseases in human. The purpose of this work is to review what is known about mycotoxin and gut microbiota interactions, the mechanisms involved in these interactions, and their practical application, and to identify knowledge gaps and future research needs.

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Detoxification of ochratoxin A by Lysobacter sp. CW239 and characteristics of a novel degrading gene carboxypeptidase cp4

Cited by 45 publications

References 42 publications

Degradation of Ochratoxin A by a UV-Mutated Aspergillus niger Strain

Degradation of Ochratoxin A by a UV-Mutated Aspergillus niger Strain

Bacillus subtilis ANSB168 Producing d-alanyl-d-alanine Carboxypeptidase Could Alleviate the Immune Injury and Inflammation Induced by Ochratoxin A

Mycotoxin and Gut Microbiota Interactions

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