Detoxification of wood hydrolysates with laccase and peroxidase from the white-rot fungus Trametes versicolor

Jönsson, Leif J.; Palmqvist, Eva; Nilvebrant, Nils‐Olof; Hahn‐Hägerdal, Bärbel

doi:10.1007/s002530051233

Cited by 364 publications

(186 citation statements)

References 28 publications

Supporting

Mentioning

170

Contrasting

Unclassified

Order By: Relevance

“…Absorbance at 280nm represents furfural, 5-hydroxymethylfurfural and phenyl ring absorption band in lignin [22]. Compared with the absorbance value at 280nm, 1.93, of the untreated acid hydrolyzate, the absorbance values of overliming treatment were decreased with the pH increasing.…”

Section: Overliming Of the Acid Hydrolyzatementioning

confidence: 92%

“…However, more researchers investigated detoxification methods to detoxify the hydrolyzate to improve fermentation efficiency. Detoxification methods include neutralization [17], overliming [18], steam stripping [19], ion-exchange [20], treatment with activated carbon [20], wood charcoal [21], and laccase and peroxidase [22]. In many instances, the most economical and widely used method of detoxification involves treatment of hydrolyzates with solid calcium hydroxide [23].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Adaptation fermentation of Pichia stipitis and combination detoxification on steam exploded lignocellulosic prehydrolyzate

Zhu¹,

Yong²,

Xu³

et al. 2009

View full text Add to dashboard Cite

Yeast Pichia stipitis CBS 5776 was developed through adaptation fermentation step by step in steam exploded corn stover prehydrolyzate because high concentration of weak acids and other inhibitors present in the prehydrolyzate could degrade the fermentability. However, the adaptability of Pichia stipitis CBS 5776 in the prehydrolyzate was so limited that steam stripping and overliming were applied to remove these inhibitors from it. Corn stover was steam exploded; the filtrate of steam exploded corn stover was hydrolyzed with dilute sulfuric acid, and then the acid hydrolyzate was detoxified and fermented by Pichia stipitis CBS 5776. Steam stripping could remove volatile compounds from the acid hydrolyzate and the filtrate. At a steam stripping time of 120min, 81% acetic acid and 59% formic acid were removed from the acid hydrolyzate, 77% acetic acid and 45% formic acid were removed from the filtrate, while furfural was stripped off completely from the acid hydrolyzate and the filtrate. Overliming could reduce the contents of furfural and phenolics present in the acid hydrolyzate, however, sugars, especially pentoses, were also removed partially. It was necessary to detoxify the acid hydrolyzate in order to ferment the sugars to ethanol. Acid hydrolyzate detoxified with a combination of steam stripping for 120 min and overliming at pH11 and 60℃ for 90 min, its fermentability was significantly improved. Xylose was consumed nearly completely in 24h with an ethanol yield of 15.92g/l, 80.34% of theoretical.

show abstract

Section: Overliming Of the Acid Hydrolyzatementioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Adaptation fermentation of Pichia stipitis and combination detoxification on steam exploded lignocellulosic prehydrolyzate

Zhu¹,

Yong²,

Xu³

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Due to its abundance in hardwood hydrolysates (Jö nsson et al 1998), 4-hydroxybenzoic acid is often used as a model compound to study the effects of phenolics. Other phenols, including vanillin, vanillic acid, hydroxybenzaldehyde, syringaldehyde, catechol, resorcinol, and salicylic acid have also been studied (as cited in Palmqvist and Hahn-Hä gerdal 2000).…”

Section: Common Inhibitors In Hydrolysates Effects and Mechanismsmentioning

confidence: 99%

“…The toxicity of phenols has been successfully reduced by the addition of laccase (EC 1.10.3.2) to hydrolysates (Jö nsson et al 1998). Expression of the Trametes versicolor laccase gene into S. cerevisiae (Larsson et al 2001a) resulted in decreased fermentation times on hydrolysates, while overexpression of a phenylacrylic-acid decarboxylase resulted in improved growth rate and ethanol productivity in dilute-acid hydrolysates (Larsson et al 2001b).…”

Section: Improving Inhibitor Tolerance Of S Cerevisiaementioning

confidence: 99%

Alcoholic fermentation of carbon sources in biomass hydrolysates by Saccharomyces cerevisiae: current status

Maris

Abbott

Bellissimi

et al. 2006

Antonie van Leeuwenhoek

436

276

View full text Add to dashboard Cite

Fuel ethanol production from plant biomass hydrolysates by Saccharomyces cerevisiae is of great economic and environmental significance. This paper reviews the current status with respect to alcoholic fermentation of the main plant biomass-derived monosaccharides by this yeast. Wild-type S. cerevisiae strains readily ferment glucose, mannose and fructose via the Embden-Meyerhof pathway of glycolysis, while galactose is fermented via the Leloir pathway. Construction of yeast strains that efficiently convert other potentially fermentable substrates in plant biomass hydrolysates into ethanol is a major challenge in metabolic engineering. The most abundant of these compounds is xylose. Recent metabolic and evolutionary engineering studies on S. cerevisiae strains that express a fungal xylose isomerase have enabled the rapid and efficient anaerobic fermentation of this pentose.L-Arabinose fermentation, based on the expression of a prokaryotic pathway in S. cerevisiae, has also been established, but needs further optimization before it can be considered for industrial implementation. In addition to these already investigated strategies, possible approaches for metabolic engineering of galacturonic acid and rhamnose fermentation by S. cerevisiae are discussed. An emerging and major challenge is to achieve the rapid transition from proof-of-principle experiments under 'academic' conditions (synthetic media, single substrates or simple substrate mixtures, absence of toxic inhibitors) towards efficient conversion of complex industrial substrate mixtures that contain synergistically acting inhibitors.

show abstract

“…In addition to the fungus described here, Schneider (18) proposed the use of a mutant yeast to remove acetic acid from hemicellulose hydrolysates. Treatment of hydrolysates with laccase and peroxidase enzymes has also been described (19).…”

Section: Discussionmentioning

confidence: 99%

Bioabatement to Remove Inhibitors from Biomass-Derived Sugar Hydrolysates

Nichols¹,

Dien²,

Guisado³

et al.

Twenty-Sixth Symposium on Biotechnology for Fuels and Chemicals

View full text Add to dashboard Cite

Bioabatement is a potential method to remove inhibitory compounds from lignocellulose hydrolysates that could be incorporated into a scheme for fermentation of ethanol from cellulose. Coniochaeta ligniaria NRRL30616, an Ascomycete that metabolizes furfural and 5-hydroxymethylfurfural, is a unique strain that may be useful for detoxifying biomass sugars. NRRL30616 and 23 related fungal strains were screened for the ability to metabolize furans and grow in dilute-acid hydrolysate of corn stover. NRRL30616 was the best strain for removal of inhibitors from hydrolysate, and abatement of hydrolysate by inoculation with the strain allowed subsequent yeast fermentation of cellulose to ethanol.

show abstract

Detoxification of wood hydrolysates with laccase and peroxidase from the white-rot fungus Trametes versicolor

Cited by 364 publications

References 28 publications

Adaptation fermentation of Pichia stipitis and combination detoxification on steam exploded lignocellulosic prehydrolyzate

Adaptation fermentation of Pichia stipitis and combination detoxification on steam exploded lignocellulosic prehydrolyzate

Alcoholic fermentation of carbon sources in biomass hydrolysates by Saccharomyces cerevisiae: current status

Bioabatement to Remove Inhibitors from Biomass-Derived Sugar Hydrolysates

Contact Info

Product

Resources

About