Detoxification with resin promotes the shift from acidogenesis to solventogenesis and prevents acid crash during butanol fermentation from wheat straw

Liu, Guoqiang; Yi, Zhuolin; Li, Jiang; Yang, Lin; Fang, Yang; Du, Anping; He, Kai; Zhao, Hai; Jin, Yanling

doi:10.1007/s13399-023-04023-0

Cited by 2 publications

(2 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although acid re-assimilation for solvent production is important in this phase, a direct pathway for butanol production called the "hot channel" has been described, which would require significant metabolic activity in this channel. In recent research, the hot channel pathway has also been identified as a significant route for butanol production without the need for acid accumulation (Jang et al, 2023;Liu et al, 2023;Mogollón et al, 2023). The high sensitivity of the parameter V 8 to acetone also suggests that the re-assimilation pathway known as the "cold channel" is active.…”

Section: Parametric Sensitivitymentioning

confidence: 99%

NADH-based kinetic model for acetone-butanol-ethanol production by Clostridium

Quintero-Díaz,

Mendoza,

Avignone-Rossa

2023

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

We present in this work a kinetic model of the acetone-butanol-ethanol (ABE) fermentation based on enzyme kinetics expressions. The model includes the effect of the co-substrate NADH as a modulating factor of cellular metabolism. The simulations obtained with the model showed an adequate fit to the experimental data reported by several authors, matching or improving the results observed with previous models. In addition, this model does not require artificial mathematical strategies such as on-off functions to achieve a satisfactory fit of the ABE fermentation dynamics. The parametric sensitivity allowed to identify the direct glucose → acetyl-CoA → butyryl-CoA pathway as being more significant for butanol production than the acid re-assimilation pathway. Likewise, model simulations showed that the increase in NADH, due to glucose concentration, favors butanol production and selectivity, finding a maximum selectivity of 3.6, at NADH concentrations above 55 mM and glucose concentration of 126 mM. The introduction of NADH in the model would allow its use for the analysis of electrofermentation processes with Clostridium, since the model establishes a basis for representing changes in the intracellular redox potential from extracellular variables.

show abstract

Section: Parametric Sensitivitymentioning

confidence: 99%

NADH-based kinetic model for acetone-butanol-ethanol production by Clostridium

Quintero-Díaz,

Mendoza,

Avignone-Rossa

2023

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Despite indications that inhibitors may be stimulatory in some cases, fermentative processing of truly crude hydrolysates results in very low solvent production [ 44 , 45 ] in the vast majority of cases. Thus, interventions are necessary, either in the composition of the fermentation medium in the form of detoxification, masking of the negative effect by the addition of other substances, or intervention in the genome of the production strain.…”

Section: Introductionmentioning

confidence: 99%

Application of fed-batch strategy to fully eliminate the negative effect of lignocellulose-derived inhibitors in ABE fermentation

Branska,

Koppova,

Husakova

et al. 2024

Biotechnol Biofuels

View full text Add to dashboard Cite

Background Inhibitors that are released from lignocellulose biomass during its treatment represent one of the major bottlenecks hindering its massive utilization in the biotechnological production of chemicals. This study demonstrates that negative effect of inhibitors can be mitigated by proper feeding strategy. Both, crude undetoxified lignocellulose hydrolysate and complex medium supplemented with corresponding inhibitors were tested in acetone–butanol–ethanol (ABE) fermentation using Clostridium beijerinckii NRRL B-598 as the producer strain. Results First, it was found that the sensitivity of C. beijerinckii to inhibitors varied with different growth stages, being the most significant during the early acidogenic phase and less pronounced during late acidogenesis and early solventogenesis. Thus, a fed-batch regime with three feeding schemes was tested for toxic hydrolysate (no growth in batch mode was observed). The best results were obtained when the feeding of an otherwise toxic hydrolysate was initiated close to the metabolic switch, resulting in stable and high ABE production. Complete utilization of glucose, and up to 88% of xylose, were obtained. The most abundant inhibitors present in the alkaline wheat straw hydrolysate were ferulic and coumaric acids; both phenolic acids were efficiently detoxified by the intrinsic metabolic activity of clostridia during the early stages of cultivation as well as during the feeding period, thus preventing their accumulation. Finally, the best feeding strategy was verified using a TYA culture medium supplemented with both inhibitors, resulting in 500% increase in butanol titer over control batch cultivation in which inhibitors were added prior to inoculation. Conclusion Properly timed sequential feeding effectively prevented acid-crash and enabled utilization of otherwise toxic substrate. This study unequivocally demonstrates that an appropriate biotechnological process control strategy can fully eliminate the negative effects of lignocellulose-derived inhibitors. Graphical Abstract

show abstract

Detoxification with resin promotes the shift from acidogenesis to solventogenesis and prevents acid crash during butanol fermentation from wheat straw

Cited by 2 publications

References 42 publications

NADH-based kinetic model for acetone-butanol-ethanol production by Clostridium

NADH-based kinetic model for acetone-butanol-ethanol production by Clostridium

Application of fed-batch strategy to fully eliminate the negative effect of lignocellulose-derived inhibitors in ABE fermentation

Contact Info

Product

Resources

About