Detrimental Impact of λ-Cyhalothrin on the Biocontrol Efficacy of Eocanthecona furcellata by Affecting Global Transcriptome and Predatory Behavior

Abstract

Whether and how insecticide exposure will affect the biological control efficacy of predatory arthropods is critical in insecticide toxicology research but largely unexplored. In the current study, reduced biocontrol efficacy was observed in a predatory stink bugEocanthecona furcellataafter insecticide application in the field. Thus, we constructed a comparative transcriptome analysis and identified a total of 4364 upregulated and 1043 down regulated differentially expressed genes following the sublethal exposure of λ-cyhalothrin. The reduced juvenile hormone (JH) titer and increased trehalose content were observed. The predation capacity and theoretical maximum predation of predators were decreased by 31.08 and 48.90% in response to λ-cyhalothrin, respectively. Furthermore, JH supplementation after λ-cyhalothrin treatment could significantly stimulate trehalase and detoxification enzyme activities, as well as restore the predatory ability of E. furcellata. Our results help to understand the toxicological mechanism of predatory stink bug species in responding to insecticides, benefit predators' ecological services, and optimize the insecticide selection.

Insulin Receptor Substrate-1 (IRS1) Regulates Oogenesis and Vitellogenesis inPropylea japonicaby Mediating theFOXO Transcription FactorExpression, Independent of JH and 20E Signaling Pathways

“…The quantification of JH and 20E titers was performed by the double-antibody sandwich method of enzyme-linked immunosorbent assay (ELISA) (Shanghai Enzyme-linked Biotechnology Co., Ltd.), as previously described. , …”