Detrusor underactivity causes neurogenic voiding dysfunction in a rat bilateral accessory nerve-injury model

Maeda, Kotomi; Hotta, Yuji; Kawata, Ryoya; Kataoka, Tomoya; Kimura, Kazunori

doi:10.1016/j.jphs.2021.03.008

Cited by 4 publications

(1 citation statement)

References 14 publications

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“…Quantitative Real-Time (qRT)-PCR qRT-PCR analysis was performed as previously reported. 18) Total RNA was extracted from RPTECs of each passage using a Pure link RNA mini kit (Thermo Fisher Scientific) according to the manufacturer's instructions. Using a ReverTra qPCR RT Master Mix with gDNA Remover (Toyobo Co., Ltd., Osaka, Japan), total RNA was reverse-transcribed into cDNA, which served as the template for PCR performed using the PowerUp SYBR Green Master Mix (Thermo Fisher Scientific).…”

Section: Methodsmentioning

confidence: 99%

Effect of Replicative Senescence on the Expression and Function of Transporters in Human Proximal Renal Tubular Epithelial Cells

Sanagawa

Hotta

Sezaki

et al. 2022

Biological & Pharmaceutical Bulletin

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In the field of cosmetic research, there is a growing interest in alternatives to animal experiments, such as in vitro models using cultured cells. The trend is spreading to the field of food and drugs. Although various types of cells are used as in vitro models, the effect of cellular senescence on the expression and function of transporters in these models is unclear. In the present study, we examined the effect of replicative senescence (by passage culture) on the expression and function of transporters in renal proximal tubular epithelial cells (RPTECs). The increase in senescence-associated-β-galactosidase (SA-β-gal)-positive cells, cell cycle arrest markers, and senescence-associated secretory phenotype (SASP) markers was associated with an increase in passage numbers of RPTECs. Gene expression of various transporters in RPTEC was also altered. The mRNA level of organic cation transporter 2 decreased most rapidly with passage numbers among the transporters. The uptake of fluorescent cationic substrates in SA-β-gal-positive RPTECs was less than that in SA-β-gal-negative RPTECs. However, these changes in the expression of transporters seem to be significantly different from those observed in rodents and human kidneys in many aspects. As cellular senescence is observed in various situations, especially in RPTECs, it may be necessary to exclude it from toxicological and pharmacokinetic evaluations using in vitro models as much as possible. Additionally, when discussing cellular senescence, it is important to note the differences between aging in cells and aging and senescence in individuals.

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Section: Methodsmentioning

confidence: 99%

Effect of Replicative Senescence on the Expression and Function of Transporters in Human Proximal Renal Tubular Epithelial Cells

Sanagawa

Hotta

Sezaki

et al. 2022

Biological & Pharmaceutical Bulletin

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Can We Differentiate Between Organic and Functional Bladder Outlet Obstruction in Males With Parkinson's Disease?

Vírseda‐Chamorro,

Salinas‐Casado,

Adot‐Zurbano

et al. 2024

Neurourology and Urodynamics

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ObjectivesTo determine the type of bladder outlet obstruction (BOO) in patients with Parkinson's disease (PD).Material and MethodA case–control study was carried out in 46 patients divided into two groups. Group 1 formed by 23 PD patients with BOO (a URA parameter ≥ 29 cm H2O). Group 2 formed by 23 patients with benign prostatic hyperplasia (BPH) and compressive obstruction (an opening pressure > 35 cm H2O) and URA parameter ≥ 29 cm H2O). Both groups underwent a pressure‐flow study to calculate Dynamic Urethral Resistance Relationship (DURR) patterns. Based on previous research, we describe two types of DURR pattern. Pattern A typical of dynamic or functional obstruction and pattern B typical of static or organic obstruction.ResultsWe found that PD patients had a significantly higher frequency of pattern A (70%) than BPH patients (4%). Other significant differences between groups were age (greater in PD group), bladder compliance (greater in PD group), maximum flow rate [Qmax (greater in BPH group)], maximum detrusor pressure [Pmax (greater in BPH group)], detrusor pressure at maximum flow rate [PQmax (greater in BPH group)], opening detrusor pressure (greater in BPH group), and the bladder contractility parameters BCI and Wmax (greater in BPH group). There were no significant differences in perineal voiding electromyography (EMG) activity between groups nor relationship between voiding EMG activity and the type of DURR pattern.ConclusionsOur results are consistent with the usefulness of the DURR pattern to differentiate between functional and organic BOO in PD patients. Most PD patients have functional obstruction although a minority has organic obstruction consistent with BPH.

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Short-term effects of suprapubic catheterization on detrusor muscle contraction in a rat model of postoperative urinary dysfunction

Kawata,

Hotta,

Kataoka

et al. 2024

Continence

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Detrusor underactivity causes neurogenic voiding dysfunction in a rat bilateral accessory nerve-injury model

Cited by 4 publications

References 14 publications

Effect of Replicative Senescence on the Expression and Function of Transporters in Human Proximal Renal Tubular Epithelial Cells

Effect of Replicative Senescence on the Expression and Function of Transporters in Human Proximal Renal Tubular Epithelial Cells

Can We Differentiate Between Organic and Functional Bladder Outlet Obstruction in Males With Parkinson's Disease?

Short-term effects of suprapubic catheterization on detrusor muscle contraction in a rat model of postoperative urinary dysfunction

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