Deuterium Depleted Water Inhibits the Proliferation of Human MCF7 Breast Cancer Cell Lines by Inducing Cell Cycle Arrest

Yavari, Kamal; Kooshesh, Lida

doi:10.1080/01635581.2019.1595048

Cited by 27 publications

(26 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We found that long-term DDW consumption by DIO rats normalized the activities of the antioxidant enzymes SOD and CAT. This observation is consistent with previous data regarding the protective effect of DDW on the antioxidant system of the body in various pathologies [18,20,26,50,64,65].…”

Section: Discussionsupporting

confidence: 93%

Deuterium-Depleted Water as Adjuvant Therapeutic Agent for Treatment of Diet-Induced Obesity in Rats

et al. 2019

View full text Add to dashboard Cite

In this study, we present the potential application of deuterium-depleted water (DDW) for the prevention and adjuvant treatment of obesity in rats. We tested the hypothesis that DDW can alleviate diet-induced obesity (DIO) and its associated metabolic impairments. Rats fed a high-fat diet had an increased body weight index (BWI), glucose concentration, and level of certain proinflammatory cytokines; decreased levels of insulin in the serum; decreased tryptophan and serotonin in the brain, and a decreased concentration of some heavy metals in the liver. Drinking DDW at a concentration of 10 ppm deuterium/protium (D/H) ad libitum for 3 weeks restored the BWI, glucose (serum), tryptophan (brain), and serotonin (brain) levels and concentration of Zn in the liver in the DIO animals to those of the controls. The levels of proinflammatory cytokines (IL-1β, IL-6, IFNγ) and anti-inflammatory TNFα were decreased in DIO rats, while anti-inflammatory cytokine (IL-4, IL-10) levels remained at the control levels, which is indicative of a pathophysiological syndrome. In contrast, in groups of rats treated with DDW, a significant increase in anti-inflammatory (IL-4, IL-10) and proinflammatory cytokines (IFNγ) was observed. This finding indicates a reduction in systemic inflammation in obese animals treated with DDW. Similarly, the high-fat diet caused an increased level of oxidative stress products, which was accompanied by decreased activity of both superoxide dismutase and catalase, whereas the administration of DDW decreased the level of oxidative stress and enhanced antioxidant enzyme activities.Molecules 2020, 25, 23 2 of 15 the development of new, safer and more effective pharmaceutical approaches for obesity treatment is essential.Natural water is a mixture of molecules containing stable isotopes 16 O, 17 O, 18 O, 1 H, and 2 H. The ratio of deuterium atoms to protium (D/H) in fresh and marine water is 132-156 µg/g (ppm) [7,8].Since the mass of protium H is half of the mass of deuterium D, effects arising from the replacement of isotopes influence biological systems; for example, the rates of chemical reactions between substances containing these isotopes may differ by a factor of 5-10 [9-11].Numerous publications over the last 20 years have been devoted to the study of the role of deuterium in natural (150 ppm) or reduced content in the human body [12]. It has been shown that deuterium-depleted water (DDW) exhibits antidotal properties with individual and combined effects on the dosage of pharmaceutical substances and auxiliary substances [13][14][15][16]. The mechanisms of such influence are due to the structure, physicochemical properties of DDW [17] and changes in ligand-receptor interactions in biological objects of different hierarchical levels [18]. DDW increases the rate of photosynthesis and promotes the growth of plants and aquatic animals [19,20]. In Europe, the USA, Japan, China and Russia, DDW is used for prophylactic and therapeutic purposes [21,22]. Currently, DDW is using as an adjuvant in cancer treatment ...

show abstract

Section: Discussionsupporting

confidence: 93%

Deuterium-Depleted Water as Adjuvant Therapeutic Agent for Treatment of Diet-Induced Obesity in Rats

et al. 2019

View full text Add to dashboard Cite

show abstract

“…One of the possible mechanisms of DDW influence on the cell cycle can be carried out through the suppression of individual genes expression. Thus it was found that the water δ 2 H = −679‰ inhibited the cells proliferation at the G0/G1 stage and the S phase cell population [105]. Moreover, female mice which consumed water δ 2 H = −839‰ showed significant suppression of 7,12-dimethylbenz[ a ]anthracene (DMBA)-induced expression of Bcl2, Kras, and Myc [52].…”

Section: Biological Effects Of Deuterium-depleted Water (Ddw)mentioning

confidence: 99%

“…At the same time the activity of antioxidant protection enzymes (catalase, superoxide dismutase) and malondialdehyde content in MCF-7 cells changed. In general, the analysis of the cell cycle shows the ability of deuterium in low concentrations to cause a cell cycle stop during the G1/S transition [105]. Also changes in prooxidant-antioxidant system functional activity of nervous tissue cells against the background of their incubating in medium with δ 2 H = −679‰ led to decrease in mitochondrial potential of cerebellar neurons and their increased mortality rate during glucose deprivation and temperature stress (39 °C) [87].…”

Section: Biological Effects Of Deuterium-depleted Water (Ddw)mentioning

confidence: 99%

Deuterium-Depleted Water Influence on the Isotope 2H/1H Regulation in Body and Individual Adaptation

et al. 2019

View full text Add to dashboard Cite

This review article presents data about the influence of deuterium-depleted water (DDW) on biological systems. It is known that the isotope abundances of natural and bottled waters are variable worldwide. That is why different drinking rations lead to changes of stable isotopes content in body water fluxes in human and animal organisms. Also, intracellular water isotope ratios in living systems depends on metabolic activity and food consumption. We found the 2H/1H gradient in human fluids (δ2H saliva >> δ2H blood plasma > δ2Hbreast milk), which decreases significantly during DDW intake. Moreover, DDW induces several important biological effects in organism (antioxidant, metabolic detoxification, anticancer, rejuvenation, behavior, etc.). Changing the isotope 2H/1H gradient from “2H blood plasma > δ2H visceral organs” to “δ2H blood plasma << δ2H visceral organs” via DDW drinking increases individual adaptation by isotopic shock. The other possible mechanisms of long-term adaptation is DDW influence on the growth rate of cells, enzyme activity and cellular energetics (e.g., stimulation of the mitochondrion activity). In addition, DDW reduces the number of single-stranded DNA breaks and modifies the miRNA profile.

show abstract

“…The deuterium concentration in the body plays an important role in the metabolic processes of living systems [ 1 , 2 , 3 , 4 ]. The biological effects caused by deuterium depleted water (DDW) have been studied at various levels: cellular [ 5 , 6 , 7 , 8 ], tissue [ 9 ], organismic [ 10 , 11 , 12 , 13 , 14 ]. It was found that low concentrations of deuterium in the drinking diet increase stress resistance in mammals [ 15 ], and may also have neuroprotective properties [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Inequality in the Frequency of the Open States Occurrence Depends on Single 2H/1H Replacement in DNA

et al. 2020

View full text Add to dashboard Cite

In the present study, the effect of 2H/1H isotopic exchange in hydrogen bonds between nitrogenous base pairs on occurrence and open states zones dynamics is investigated. These processes are studied using mathematical modeling, taking into account the number of open states between base pairs. The calculations of the probability of occurrence of open states in different parts of the gene were done depending on the localization of the deuterium atom. The mathematical modeling study demonstrated significant inequality (dependent on single 2H/1H replacement in DNA) among three parts of the gene similar in length of the frequency of occurrence of the open states. In this paper, the new convenient approach of the analysis of the abnormal frequency of open states in different parts of the gene encoding interferon alpha 17 was presented, which took into account both rising and decreasing of them that allowed to make a prediction of the functional instability of the specific DNA regions. One advantage of the new algorithm is diminishing the number of both false positive and false negative results in data filtered by this approach compared to the pure fractile methods, such as deciles or quartiles.

show abstract

Deuterium Depleted Water Inhibits the Proliferation of Human MCF7 Breast Cancer Cell Lines by Inducing Cell Cycle Arrest

Cited by 27 publications

References 43 publications

Deuterium-Depleted Water as Adjuvant Therapeutic Agent for Treatment of Diet-Induced Obesity in Rats

Deuterium-Depleted Water as Adjuvant Therapeutic Agent for Treatment of Diet-Induced Obesity in Rats

Deuterium-Depleted Water Influence on the Isotope 2H/1H Regulation in Body and Individual Adaptation

Inequality in the Frequency of the Open States Occurrence Depends on Single 2H/1H Replacement in DNA

Contact Info

Product

Resources

About