Deuterium magnetic resonance of selectively deuterated cholesteryl esters in dipalmitoyl phosphatidylcholine dispersions

Seipin performs dissectible functions in promoting lipid droplet biogenesis and regulating droplet morphology

“…We found several lines of evidence demonstrating that, in the absence of seipin, LD biogenetic defects are accompanied by NL accumulation within the ER membrane. Biophysical studies and calculated predictions have indeed determined that NL can accumulate within a membrane bilayer up to 3–7 mass% before becoming unstable and “oiling out” ( Gorrissen et al ., 1981 , 1982 ; Hamilton and Small, 1981 ; Mackinnon et al ., 1992 ; King et al ., 1994 ; Khandelia et al ., 2010 ). We have therefore concluded that seipin functions in facilitating the release of NL from the ER into LDs and that, in the absence of this function, NL accumulates until it blebs stochastically from the ER.…”

Physicochemical Determinations of Sterols by Deuterium and Carbon-13 Nuclear Magnetic Resonance

“…The result of such calculations can be represented using computer-assisted spatial drawing routines such as PLUTO 78; the location of the axis of motional averaging for the fused-ring system of cholesterol and cholesteryl palmitate, in dipalmitoyl phosphatidylcholine (DPPC), is represented in Fig. The palmitoyl chain is indeed known to fold back into the membrane (Gorrissen et al, 1981) and therefore "pulls" the rigid cholesteryl skeleton away from the average orientation found for cholesterol. One sees clearly that the steroid skeleton of cholesteryl palmitate is markedly tilted away from the position of that of cholesterol.…”

Studies on phytosterol acetate esters and phytosterols liposomes

“…The average particle size of all liposomes was approximately 60 nm. It was found that the incorporation of phytosterol esters did not significantly change (Gorrissen et al, 1980) or decreased the particle size of liposomes (Wang et al, 2017). The incorporated phytosterol esters molecules may replace some phospholipid molecules or form lipid rafts in the membrane, leading to tighter packing of the membrane lipids, which in turn reduces or does not significantly change the average particle diameter of liposomes (Wang et al, 2017).…”