“…The ability to sense and respond to intracellular metabolites is crucial for better understanding and regulating pathway behavior for enhanced metabolite production [ 1 ]. Many different types of biosensors have been developed and characterized in microbial hosts, including aptamers, riboswitches, fluorescence resonance energy transfer (FRET)-based sensors, and transcription factor (TF)-based sensors [ 2 , 3 , 4 , 5 ]. Aptamers are nucleic-acid-based biosensors that undergo a conformational change upon the binding of a target metabolite.…”