The experiment of the hardness property for 65Mn spring steel under different heat treatments is conducted. The results are discussed based on the regression orthogonal design. The hardness property of 65Mn steel has been discussed by applying diverse heat treatment technology and the optimum parameters of heat treatment for the best hardness value are obtained through experiments. The influence of the parameters on hardness property is studied by applying the regression orthogonal design. The relation between the hardness and diverse heat treatment parameters has been given by using planar figure. The optimum heat treatment parameter for maximum hardness ability is obtained as following: 851.4°C for quenching temperature, 18 min for quenching time, and 146.4°C for tempering temperature, respectively. The result showed that the selection of material heat treatment process parameters has a great influence on the hardness of the material, which will provide a reliable basis to further study the wear resistance of material.