Developing a SNP panel for forensic identification of individuals

Kídd, Kenneth K.; Pakstis, Andrew J.; Speed, William C.; Grigorenko, Elena L.; Kajuna, Sylvester L.B.; Karoma, Nganyirwa J.; Kungulilo, Selemani; Kim, Jong-Jin; Lu, Ru‐Band; Odunsi, Adekunle; Okonofua, Friday; Parnas, Josef; Schulz, Leslie O.; Жукова, О. В.; Kidd, Judith R.

doi:10.1016/j.forsciint.2005.11.017

Cited by 241 publications

(163 citation statements)

References 19 publications

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“…SNPs for individual, phenotypic, and species identification, as well as, ancestry and parentage analysis are available in cats and can be assayed by various techniques 9, 44, 45, 46, 47. Hence, this study embarked upon developing an effective SNP identification panel that can be easily adapted into forensic laboratories for use with cat evidence.…”

Section: Discussionmentioning

confidence: 99%

SNP Miniplexes for Individual Identification of Random‐Bred Domestic Cats

Brooks

Creighton

Gandolfi

et al. 2016

Journal of Forensic Sciences

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Phenotypic and genotypic characteristics of the cat can be obtained from single nucleotide polymorphisms (SNPs) analyses of fur. This study developed miniplexes using SNPs with high discriminating power for random‐bred domestic cats, focusing on individual and phenotypic identification. Seventy‐eight SNPs were investigated using a multiplex PCR followed by a fluorescently labeled single base extension (SBE) technique (SNaPshot®). The SNP miniplexes were evaluated for reliability, reproducibility, sensitivity, species specificity, detection limitations, and assignment accuracy. Six SNPplexes were developed containing 39 intergenic SNPs and 26 phenotypic SNPs, including a sex identification marker, ZFXY. The combined random match probability (cRMP) was 6.58 × 10−19 across all Western cat populations and the likelihood ratio was 1.52 × 1018. These SNPplexes can distinguish individual cats and their phenotypic traits, which could provide insight into crime reconstructions. A SNP database of 237 cats from 13 worldwide populations is now available for forensic applications.

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Section: Discussionmentioning

confidence: 99%

SNP Miniplexes for Individual Identification of Random‐Bred Domestic Cats

Brooks

Creighton

Gandolfi

et al. 2016

Journal of Forensic Sciences

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“…The stability of single nucleotide positions compared to STRs [3,4] makes SNPs attractive for relationship testing, where germline mutations must be considered routinely. Pakstis and Kidd et al have identified panels of human autosomal SNPs with a uniform distribution of heterozygosity across global populations, making them ideal for forensic applications [5][6][7].…”

Section: Snp Genotyping Assaymentioning

confidence: 99%

“…Ibis Biosciences has developed SNP genotyping assays for the PLEX-ID TM mass spectrometry-based platform according to the panels identified by Pakstis and Kidd, et al [5][6][7]. The Ibis platform fully automates analysis of PCR-amplified products and processes up to 15 assay plates in a single run with multiple assays, including mtDNA profiling, SNPs and STRs [8][9][10].…”

Section: Snp Genotyping Assaymentioning

confidence: 99%

Human autosomal SNP profiling using fully automated electrospray ionization time of flight mass spectrometry

Hall

Marzan

Kikpatrick

et al. 2011

Forensic Science International: Genetics Supplement Series

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“…Under identity testing, SNPs are prominently used for paternity testing, mass disaster and missing persons [3][4][5]. A complicated scenario is when SNPs are intended for identification in a mixture, this has been a subject of debate as to their use for mixtures.…”

Section: Introductionmentioning

confidence: 99%

Identification of Individuals in a DNA Mixture Using SNP Markers

Ibrahim¹

2017

IJGG

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Abstract:This article focuses mainly on DNA mixture from two contributors, a victim and an unknown culprit. There are two areas I believe will be of interest to forensic scientists, police and a Jury. These areas are identification of an individual in a DNA mixture and familial DNA database searching of a culprit through a relative. In this article, I looked at identification of individuals in a mixture using Single Nucleotide Polymorphisms (SNPs) markers. SNPs are starting to be used for forensic identification; I employed them as they produce incredible results for identification in a two-person mixture. The conservative method I employed here is the random man not excluded probability -P (RMNE) approach, an inclusion probability method generally considered as a frequentist approach. It was found that an optimum allele frequency of 0.2 is required to produce almost certain identification with much distortion in identifying an individual even when inbreeding is up to 50% in a population. Another interesting thing is that relatives of a suspect whom are actual contributors to the DNA mixture can also be identified. In a case where there are relatives in the mixture it was found that twice the number of SNP panels is required to identify an individual than in a case where no relative is involved. And lastly, typing more SNP panels helps to improve identification and therefore produce forensically useful results.

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Developing a SNP panel for forensic identification of individuals

Cited by 241 publications

References 19 publications

SNP Miniplexes for Individual Identification of Random‐Bred Domestic Cats

SNP Miniplexes for Individual Identification of Random‐Bred Domestic Cats

Human autosomal SNP profiling using fully automated electrospray ionization time of flight mass spectrometry

Identification of Individuals in a DNA Mixture Using SNP Markers

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