Developing a tissue glue by engineering the adhesive and hemostatic properties of metal oxide nanoparticles

Abstract

Despite decades of research, wound complications remain a major cause of postoperative mortality, especially in the face of multiple comorbidities. Addressing the issue of anastomotic leakages and impaired wound healing from a new angle is of great interest with the prospect of having direct impact on patient outcome. Recently, aqueous suspensions of silica and iron oxide nanoparticles have been employed to connect biological tissue by serving as an adhesive layer eventually leading to macroscopic gluing of tissue. In this work, we explore the prospects of this effect by introducing bioactive tissue adhesives composed of nanoparticles produced via scalable and sterile flame spray pyrolysis. We investigate six different metal oxides on cytocompatibility, hemostatic activity and adhesive properties in a small intestine lap joint model. While bioglass nanoparticles show exceptionally strong procoagulant and adhesive properties, the cell membrane integrity is impaired at high particle concentrations. Interestingly, when bioglass is combined with ceria, a material that has well-documented cytoprotective effects, the resulting hybrid particles exhibit the same beneficiary effects as bioglass while featuring superior cytocompatibility. Taken together, we demonstrate highly modular synthesis of nanoparticles expressing adhesive properties in conjunction with tailored bioactivity. Such bioactive nanoparticles as adhesion nuclei in wound healing have a wide range of potential applications in surgical wound care and regenerative medicine.

Coercivity Determines Magnetic Particle Heating

“…THP‐1 cells were cultivated, seeded, and incubated with nanoparticles according to previous work . After incubation, the contents of the wells were extracted and centrifuged at 6000 g for 5 min.…”

Tannic Acid Cross‐linked Polysaccharide‐Based Multifunctional Hemostatic Microparticles for the Regulation of Rapid Wound Healing

“…Negative zeta potential was caused by the carboxymethyl group in CMC. It was reported that HMs with negative zeta potential could activate blood coagulation factor XII and play important roles in rapid hemostasis …”

Antibacterial and biodegradable tissue nano-adhesives for rapid wound closure

“…In addition to improving the mechanical properties and adhesive strength by the synthetic polymers, [8][9][10][11] inorganic nanoparticles (NPs) themselves are known as tissue adhesives owing to the so-called "nanobridging effect" between numerous protein chains in the wound. [12][13][14][15][16][17][18] It has been recently reported that several inorganic NPs including silica NPs, magnetic NPs and metal NPs exhibit strong adhesive properties in the wound closure of animal models. [13][14][15][16][17][18] This novel strategy is advantageous in terms of cost, efficiency and convenience for clinical translation when compared with traditional polymer-based tissue adhesion methods.…”

“…[12][13][14][15][16][17][18] It has been recently reported that several inorganic NPs including silica NPs, magnetic NPs and metal NPs exhibit strong adhesive properties in the wound closure of animal models. [13][14][15][16][17][18] This novel strategy is advantageous in terms of cost, efficiency and convenience for clinical translation when compared with traditional polymer-based tissue adhesion methods. 12 However, a few studies have reported the antibacterial effects of these inorganic tissue adhesives.…”