2017
DOI: 10.1021/acsami.7b09992
|View full text |Cite
|
Sign up to set email alerts
|

Developing an Inositol-Phosphate-Actuated Nanochannel System by Mimicking Biological Calcium Ion Channels

Abstract: In eukaryotic cells, ion channels, which ubiquitously present as polypeptides or proteins, usually regulate the ion transport across biological membranes by conformational switching of the channel proteins in response to the binding of diverse signaling molecules (e.g., inositol phosphate, abbreviated to InsP). To mimic the gating behaviors of natural Ca channels manipulated by InsPs, a smart poly[(N-isopropylacrylamide-co-4-(3-acryloylthioureido) benzoic acid)] (denoted as PNI-co-ATBA) was integrated onto a p… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
18
0

Year Published

2018
2018
2024
2024

Publication Types

Select...
7

Relationship

2
5

Authors

Journals

citations
Cited by 25 publications
(18 citation statements)
references
References 71 publications
0
18
0
Order By: Relevance
“…Then, electrolytes with different concentrations of CaCl 2 were separately added to the cell exposed to the PAA membrane. Figure 5b displays the CaCl 2 concentration dependence of the ionic current (at +0.2 V) change ratio [defined as (I − I 0 )/I 0 , where I 0 is the initial current] for the bare, PNIPAAm and PNI-co-CF 3 -PT 0.2 -co-DDDEEKC 0.2 -modified PAA membranes 45 . The detection range for Ca 2+ extended from 1 × 10 −4 mol L −1 to an ultratrace level of 1 × 10 −11 mol L −1 , which fully covered the intracellular and extracellular Ca 2+ / 1 × 10 −8 to 1 × 10 −3 mol L −157,58 and makes it possible to further utilize this artificial nanochannel for medical treatment.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…Then, electrolytes with different concentrations of CaCl 2 were separately added to the cell exposed to the PAA membrane. Figure 5b displays the CaCl 2 concentration dependence of the ionic current (at +0.2 V) change ratio [defined as (I − I 0 )/I 0 , where I 0 is the initial current] for the bare, PNIPAAm and PNI-co-CF 3 -PT 0.2 -co-DDDEEKC 0.2 -modified PAA membranes 45 . The detection range for Ca 2+ extended from 1 × 10 −4 mol L −1 to an ultratrace level of 1 × 10 −11 mol L −1 , which fully covered the intracellular and extracellular Ca 2+ / 1 × 10 −8 to 1 × 10 −3 mol L −157,58 and makes it possible to further utilize this artificial nanochannel for medical treatment.…”
Section: Resultsmentioning
confidence: 99%
“…A piece of PAA membrane (bare or modified) was mounted between a two-compartment electrochemical cell according to the literature 45 . Ag/AgCl electrodes were used to apply a transmembrane potential across the membrane.…”
Section: Ionic Current Measurementmentioning
confidence: 99%
“…Inositol. Inspired by Ca 2+ ionic channels, a biomimetic inositol phosphate‐responsive nanopore device was built on AAO membranes . Inositol phosphate (InsP) is a signaling molecule and can be specifically recognized in the nanomolar range by a N ‐isopropylacrylamide‐ co ‐4‐(3‐acryloylthioureido) benzoic acid copolymer (PNI‐ co ‐ATBA) by hydrogen bond formation with the ATBA moieties.…”
Section: Rational Integration Of (Bio)molecular Architectures Into Namentioning
confidence: 99%
“…NIPAAm-co-ATBA 0.35 @SiO 2 was prepared through a typical surface-initiated atom transfer radical polymerization (SI-ATRP) according to the literature [34]. The rationality of the copolymer design has been validated in our previous work [27].…”
Section: Synthesis Of Nipaam-co-atba 035 @Siomentioning
confidence: 99%
“…Furthermore, for the fabrication of novel phosphoprotein enrichment material, the developed responsive copolymer was grafted onto the porous silica gel (diameter 5 μm, inner pore size 350 Å) through SI-ATRP [34]. The copolymer-modified silica gel (denoted as NIPAAm-co-ATBA 0.35 @SiO 2 ) was characterized through scanning electron microscopy (SEM), nitrogen isothermal adsorption, thermal gravimetric analysis (TGA) and organic element analysis and zeta-potential analysis.…”
Section: Fabrication and Characterization Of The Nipaam-co-atba 035 mentioning
confidence: 99%