Developing and quality testing of microsatellite loci for four species of Glossina

Ravel, Sophie; Séré, Modou; Manangwa, Oliver; Kagbadouno, Moise S.; Mahamat, Mahamat Hisséne; Shereni, William; Okeyo, Winnie A.; Argilés-Herrero, Rafael; Meeûs, Thierry De

doi:10.1016/j.meegid.2020.104515

Cited by 6 publications

(5 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A total of nine di-nucleotidic microsatellite loci were used (GFF3, GFF4, GFF8, GFF12, GFF16, GFF18, GFF21, GFF23, GFF27) with primers designed from a previously built microsatellite bank of G. f. fuscipes (Ravel et al, 2020). All the markers selected were autosomal (i.e.…”

Section: Microsatellite Markersmentioning

confidence: 99%

Population genetics of Glossina fuscipes fuscipes from southern Chad

Ravel¹,

Mahamat²,

Ségard³

et al. 2023

Peer Community Journal

Self Cite

View full text Add to dashboard Cite

In Subsaharan Africa, tsetse flies (genus Glossina) are vectors of trypanosomes causing Human African Trypanosomiasis (HAT) and Animal African Trypanosomosis (AAT). Some foci of HAT persist in Southern Chad, where a program of tsetse control was started against the local vector Glossina fuscipes fuscipes in the Mandoul focus in 2014, and in Maro in 2018. Flies were also sampled in 2018 in Timbéri and Dokoutou. We analyzed the population genetics of G. fuscipes fuscipes from the four tsetse-infested zones. The trapping samples were characterized by a strong female biased sex-ratio, except in Timbéri and Dokoutou that had high tsetse densities. Apparent density and effective population density appeared smaller in the main foci of Mandoul and Maro and the average dispersal distance (within the spatial scale of each zone) was as large as or larger than the total length of each respective zone. The genetic signature of a population bottleneck was found in the Mandoul and Timbéri area, suggesting a large ancient interconnected metapopulation that underwent genetic subdivision into small, isolated pockets due to adverse environmental conditions. The long-range dispersal and the existence of genetic outliers suggest a possibility of migration from remote sites such as the Central African Republic in the south (although the fly situation remains unknown there) and/or a genetic signature of recent exchanges. Due to likely isolation, an eradication strategy may be considered for sustainable HAT control in Mandoul focus. Another strategy will probably be required in Maro focus, which probably experiences much more exchanges with its neighbors.

show abstract

Section: Microsatellite Markersmentioning

confidence: 99%

Population genetics of Glossina fuscipes fuscipes from southern Chad

Ravel¹,

Mahamat²,

Ségard³

et al. 2023

Peer Community Journal

Self Cite

View full text Add to dashboard Cite

show abstract

“…Three legs of each tsetse fly were subjected to chelex treatment to obtain DNA for genotyping (Ravel et al, 2007). Genotyping was undertaken for 11 loci: X55-3 (Solano et al, 1997), XpGp13, pGp24 (Luna et al, 2001), GPCAG (Baker & Krafsur, 2001), pGp15, pGp16, and pGp27 (Ravel et al, 2020). Loci A10, B3, XB104, C102 were kindly provided by A. Robinson (Insect Pest Control Sub-program, Joint Food and Agriculture Organization of the United Nations/International Atomic Energy Agency Program of Nuclear Techniques in Food and Agriculture).…”

Section: Methodsmentioning

confidence: 99%

“…Three legs of each tsetse fly were subjected to chelex treatment to obtain DNA for genotyping (Ravel et al, 2007). Genotyping was undertaken for 11 loci: X55-3 (Solano et al, 1997), XpGp13, pGp24 (Luna et al, 2001, GPCAG (Baker & Krafsur, 2001), pGp15, pGp16, and pGp27 (Ravel et al, 2020) (Camara et al, 2006;Bouyer et al, 2007;Solano et al, 2009;Melachio et al, 2011), information about these loci were never published. Readers will find this information in Table A1 in the Appendix.…”

Section: Genotypingmentioning

confidence: 99%

Population genetics ofGlossina palpalis gambiensisin the sleeping sickness focus of Boffa (Guinea) before and after eight years of vector control: no effect of control despite a significant decrease of human exposure to the disease

Kagbadouno¹,

Modoui

Ségard³

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Human African trypanosomosis (HAT), aka sleeping sickness, is still a major concern in endemic countries. Its cyclical vector are biting insects of the genus Glossina or tsetse flies. In Guinea, the mangrove ecosystem contains the main HAT foci of Western Africa. There, the cyclical vector is Glossina palpalis gambiensis. A still ongoing vector control campaign (VCC) started in 2011 in the focus of Boffa, using tiny targets, with a significant impact on the prevalence of the disease. To assess the sustainability of these results, we have studied the impact of this VCC on the population biology of G. p. gambiensis in Boffa. We used the genotyping at several microsatellite markers and population genetics tools of tsetse flies from different sites and at different dates before and after the beginning of the VCC. In variance with a significant impact of VCC on the apparent densities of flies captured in the traps deployed, the global population of G. p. gambiensis displayed no genetic signature of control, and behaved as a very large population occupying the entire zone. This implies that targets deployment efficiently protects the human populations locally, but not where targets cannot be deployed and where the main tsetse population exploits available resources. This also entails the pursuit of vector control measures until HAT can be considered as entirely eradicated from the focus.

show abstract

“…We used a total of nine di-nucleotidic microsatellite loci (GFF3, GFF4, GFF8, GFF12, GFF16, GFF18, GFF21, GFF23, and GFF27) with primers designed from a previously built microsatellite bank of G. f. fuscipes [51]. All markers were autosomal (i.e., not on the X chromosome).…”

Section: Microsatellite Markersmentioning

confidence: 99%

Limited impact of vector control on the population genetic structure ofGlossina fuscipes fuscipesfrom the sleeping sickness focus of Maro, Chad

Ravel,

Ségard,

Mollo

et al. 2024

Parasite

Self Cite

View full text Add to dashboard Cite

Tsetse flies (genus Glossina) transmit deadly trypanosomes to human populations and domestic animals in sub-Saharan Africa. Some foci of Human African Trypanosomiasis due to Trypanosoma brucei gambiense (g-HAT) persist in southern Chad, where a program of tsetse control was implemented against the local vector Glossina fuscipes fuscipes in 2018 in Maro. We analyzed the population genetics of G. f. fuscipes from the Maro focus before control (T0), one year (T1), and 18 months (T2) after the beginning of control efforts. Most flies captured displayed a local genetic profile (local survivors), but a few flies displayed outlier genotypes. Moreover, disturbance of isolation by distance signature (increase of genetic distance with geographic distance) and effective population size estimates, absence of any genetic signature of a bottleneck, and an increase of genetic diversity between T0 and T2 strongly suggest gene flows from various origins, and a limited impact of the vector control efforts on this tsetse population. Continuous control and surveillance of g-HAT transmission is thus recommended in Maro. Particular attention will need to be paid to the border with the Central African Republic, a country where the entomological and epidemiological status of g-HAT is unknown.

show abstract

Developing and quality testing of microsatellite loci for four species of Glossina

Cited by 6 publications

References 70 publications

Population genetics of Glossina fuscipes fuscipes from southern Chad

Population genetics of Glossina fuscipes fuscipes from southern Chad

Population genetics ofGlossina palpalis gambiensisin the sleeping sickness focus of Boffa (Guinea) before and after eight years of vector control: no effect of control despite a significant decrease of human exposure to the disease

Limited impact of vector control on the population genetic structure ofGlossina fuscipes fuscipesfrom the sleeping sickness focus of Maro, Chad

Contact Info

Product

Resources

About