Developing aptasensors for forensic analysis

Gooch, James; Daniel, Barbara; Parkin, Mark C.; Frascione, Nunzianda

doi:10.1016/j.trac.2017.07.019

Cited by 43 publications

(22 citation statements)

References 117 publications

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“…Biosensors are innovative methods that can be used in the field of antimicrobial residue detection. They have been developed for dozens of years for clinical and forensic applications [14][15][16][17]. Food safety applications of biosensors that are more recent could benefit from these developments.…”

Section: Biosensorsmentioning

confidence: 99%

The Growing Interest in Development of Innovative Optical Aptasensors for the Detection of Antimicrobial Residues in Food Products

Gaudin¹

2020

Biosensors

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The presence of antimicrobial residues in food-producing animals can lead to harmful effects on the consumer (e.g., allergies, antimicrobial resistance, toxicological effects) and cause issues in food transformation (i.e., cheese, yogurts production). Therefore, to control antimicrobial residues in food products of animal origin, screening methods are of utmost importance. Microbiological and immunological methods (e.g., ELISA, dipsticks) are conventional screening methods. Biosensors are an innovative solution for the development of more performant screening methods. Among the different kinds of biosensing elements (e.g., antibodies, aptamers, molecularly imprinted polymers (MIP), enzymes), aptamers for targeting antimicrobial residues are in continuous development since 2000. Therefore, this review has highlighted recent advances in the development of aptasensors, which present multiple advantages over immunosensors. Most of the aptasensors described in the literature for the detection of antimicrobial residues in animal-derived food products are either optical or electrochemical sensors. In this review, I have focused on optical aptasensors and showed how nanotechnologies (nanomaterials, micro/nanofluidics, and signal amplification techniques) largely contribute to the improvement of their performance (sensitivity, specificity, miniaturization, portability). Finally, I have explored different techniques to develop multiplex screening methods. Multiplex screening methods are necessary for the wide spectrum detection of antimicrobials authorized for animal treatment (i.e., having maximum residue limits).

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Section: Biosensorsmentioning

confidence: 99%

The Growing Interest in Development of Innovative Optical Aptasensors for the Detection of Antimicrobial Residues in Food Products

Gaudin¹

2020

Biosensors

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“…As it is known, aptamers are single-stranded DNA or RNA oligonucleotides synthesized by a combinatorial selection process called SELEX (Systematic Evolution of Ligands by EXponential enrichment) [16]. The development of aptasensors for forensic analysis was revised by Gooch et al [17]. Important practical advantages of these molecules are high stability and easy modification.…”

Section: Inorganic Poisons: Arsenic and Cyanidementioning

confidence: 99%

What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications

et al. 2019

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This article critically discusses the latest advances in the use of voltammetric, amperometric, potentiometric, and impedimetric biosensors for forensic analysis. Highlighted examples that show the advantages of these tools to develop methods capable of detecting very small concentrations of analytes and provide selective determinations through analytical responses, without significant interferences from other components of the samples, are presented and discussed, thus stressing the great versatility and utility of electrochemical biosensors in this growing research field. To illustrate this, the determination of substances with forensic relevance by using electrochemical biosensors reported in the last five years (2015–2019) are reviewed. The different configurations of enzyme or affinity biosensors used to solve analytical problems related to forensic practice, with special attention to applications in complex samples, are considered. Main prospects, challenges to focus, such as the fabrication of devices for rapid analysis of target analytes directly on-site at the crime scene, or their widespread use and successful applications to complex samples of interest in forensic analysis, and future efforts, are also briefly discussed.

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“…Forensic science has benefited from SELEX technology, and great progress has been made to design aptasensors for forensic analysis [22]. As summarized in Table S1, DNA or RNA aptamers have been developed against a few abused drugs.…”

Section: Selection and Characterization Of Aptamers Against Abused Drugsmentioning

confidence: 99%

“…Despite their immense potential in biological diagnostics at POC, aptamer-based biosensors have yet to find their place in forensic sciences for number of reasons including very few aptamer sequences have been developed for relevant targets [20], the need for successful real-life samples application [13], and already-spent money and facilities for antibody-based detection techniques in analytical laboratories [21,22]. In this review, a bottom-up approach for developing aptasensors in the field of forensics is followed by describing the recent technologies used in custom-tailored aptamer development strategies for relevant analytes and presenting their applications in biosensors for the determination of abused drugs.…”

Section: Introductionmentioning

confidence: 99%

A Bottom-Up Approach for Developing Aptasensors for Abused Drugs: Biosensors in Forensics

et al. 2019

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Aptamer-based point-of-care (POC) diagnostics platforms may be of substantial benefit in forensic analysis as they provide rapid, sensitive, user-friendly, and selective analysis tools for detection. Aptasensors have not yet been adapted commercially. However, the significance of the applications of aptasensors in the literature exceeded their potential. Herein, in this review, a bottom-up approach is followed to describe the aptasensor development and application procedure, starting from the synthesis of the corresponding aptamer sequence for the selected analyte to creating a smart surface for the sensitive detection of the molecule of interest. Optical and electrochemical biosensing platforms, which are designed with aptamers as recognition molecules, detecting abused drugs are critically reviewed, and existing and possible applications of different designs are discussed. Several potential disciplines in which aptamer-based biosensing technology can be of greatest value, including forensic drug analysis and biological evidence, are then highlighted to encourage researchers to focus on developing aptasensors in these specific areas.

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Developing aptasensors for forensic analysis

Cited by 43 publications

References 117 publications

The Growing Interest in Development of Innovative Optical Aptasensors for the Detection of Antimicrobial Residues in Food Products

The Growing Interest in Development of Innovative Optical Aptasensors for the Detection of Antimicrobial Residues in Food Products

What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications

A Bottom-Up Approach for Developing Aptasensors for Abused Drugs: Biosensors in Forensics

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