Developing Chenopodium ficifolium as a potential B genome diploid model system for genetic characterization and improvement of allotetraploid quinoa (Chenopodium quinoa)

Subedi, Madhav; Neff, Erin; Davis, Thomas M.

doi:10.1186/s12870-021-03270-5

Cited by 11 publications

(10 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This difference may be relevant to the adaptation of the Canadian accession to local wet growing conditions. Similar promising observations have been made through the sequencing of wild C. ficifolium , and comparisons of the flowering locus genes [ 9 ]. Our results support the utility of sequencing and assembling genomes of additional wild Chenopodium species.…”

Section: Discussionsupporting

confidence: 65%

See 1 more Smart Citation

Genomic Sequence of Canadian Chenopodium berlandieri: A North American Wild Relative of Quinoa

Samuels

Lapointe

Halwas

et al. 2023

Plants

View full text Add to dashboard Cite

Chenopodium berlandieri (pitseed goosefoot) is a widespread native North American plant, which was cultivated and consumed by indigenous peoples prior to the arrival of European colonists. Chenopodium berlandieri is closely related to, and freely hybridizes with the domesticated South American food crop C. quinoa. As such it is a potential source of wild germplasm for breeding with C. quinoa, for improved quinoa production in North America. The C. berlandieri genome sequence could also be a useful source of information for improving quinoa adaptation. To this end, we first optimized barcode markers in two chloroplast genes, rbcL and matK. Together these markers can distinguish C. berlandieri from the morphologically similar Eurasian invasive C. album (lamb’s quarters). Second, we performed whole genome sequencing and preliminary assembly of a C. berlandieri accession collected in Manitoba, Canada. Our assembly, while fragmented, is consistent with the expected allotetraploid structure containing diploid Chenopodium sub-genomes A and B. The genome of our accession is highly homozygous, with only one variant site per 3–4000 bases in non-repetitive sequences. This is consistent with predominant self-fertilization. As previously reported for the genome of a partly domesticated Mexican accession of C. berlandieri, our genome assembly is similar to that of C. quinoa. Somewhat unexpectedly, the genome of our accession had almost as many variant sites when compared to the Mexican C. berlandieri, as compared to C. quinoa. Despite the overall similarity of our genome sequence to that of C. quinoa, there are differences in genes known to be involved in the domestication or genetics of other food crops. In one example, our genome assembly appears to lack one functional copy of the SOS1 (salt overly sensitive 1) gene. SOS1 is involved in soil salinity tolerance, and by extension may be relevant to the adaptation of C. berlandieri to the wet climate of the Canadian region where it was collected. Our genome assembly will be a useful tool for the improved cultivation of quinoa in North America.

show abstract

Section: Discussionsupporting

confidence: 65%

“…Given that C. berlandieri is native to Canada, and that C. berlandieri appears to share the nutritional advantages of quinoa [ 8 ], the idea of re-domesticating it for cultivation is intriguing. A similar idea has been proposed for the diploid native North American C. ficifolium [ 9 ].…”

Section: Introductionmentioning

confidence: 94%

Genomic Sequence of Canadian Chenopodium berlandieri: A North American Wild Relative of Quinoa

Samuels

Lapointe

Halwas

et al. 2023

Plants

View full text Add to dashboard Cite

show abstract

“…Chenopodium ficifolium was proposed as a potential diploid model species for the genetic analyses of the tetraploid crop quinoa (Subedi et al ., 2021). We have therefore transformed Arabidopsis with the inducible CqFTL2-1 gene of quinoa to see whether this would result in the same outcome as the transfer of its C. ficifolium ortholog.…”

Section: Discussionmentioning

confidence: 99%

TheFLOWERING LOCUS T LIKE 2-1gene ofChenopodiumtriggers precocious flowering in Arabidopsis seedlings

Abeyawardana

Moravec

Krüger

et al. 2022

Preprint

View full text Add to dashboard Cite

The FLOWERING LOCUS T (FT) gene is the essential integrator of flowering regulatory pathways in angiosperms. The paralogs of the FT gene may perform antagonistic functions, as exemplified by BvFT1, that suppresses flowering in Beta vulgaris, unlike the paralogous activator BvFT2. The roles of FT genes in other amaranths were less investigated. Here, we transformed Arabidopsis thaliana with the FLOWERING LOCUS T like (FTL) genes of Chenopodium and found, that both FTL1 and FTL2-1 accelerated flowering, despite having been the homologs of the Beta vulgaris floral promoter and suppressor, respectively. The floral promotive effect of FTL2-1 was so strong that it caused lethality when overexpressed under the 35S promoter. FTL2-1 placed in inducible cassette accelerated flowering after the induction with methoxyphenozide. The occasional expression of FTL2-1 led to precocious flowering in some primary transformants even without chemical induction. After the FTL gene duplication in Amaranthaceae, the FTL1 copy maintained the role of floral activator. The second copy FTL2 underwent subsequent duplication and functional diversification, which enabled to control the onset of flowering in amaranths to adapt to variable environments.

show abstract

“…These genes could be either functional candidate genes with an identified or predicted function or positional candidate genes that are co-localized with a trait locus ( Pflieger et al, 2001 ). As an example, the flowering time trait of many angiosperm species is functionally related to Flowering Locus T (FT) gene homologs ( Pin and Nilsson, 2012 ; Subedi et al, 2021 ) and can be utilized for the study of the trait in any other flowering species. Positional candidate genes used for CGA can be genes identified through QTL mapping, marker-trait association, or syntenic regions between different genomes ( Salentijn et al, 2007 ).…”

Section: Breeding Methods For End-use Quality Traits Of Wheatmentioning

confidence: 99%

“…This would also be beneficial to neglected and underutilized, yet nutritionally important crops that are being left behind from genetic improvement such as quinoa, finger millet etc. ( Subedi, 2020 ; Gautam et al, 2021 ).…”

Section: Challenges and Future Opportunities For Improving Wheat End-...mentioning

confidence: 99%

Wheat end-use quality: State of art, genetics, genomics-assisted improvement, future challenges, and opportunities

et al. 2023

Self Cite

View full text Add to dashboard Cite

Wheat is the most important source of food, feed, and nutrition for humans and livestock around the world. The expanding population has increasing demands for various wheat products with different quality attributes requiring the development of wheat cultivars that fulfills specific demands of end-users including millers and bakers in the international market. Therefore, wheat breeding programs continually strive to meet these quality standards by screening their improved breeding lines every year. However, the direct measurement of various end-use quality traits such as milling and baking qualities requires a large quantity of grain, traits-specific expensive instruments, time, and an expert workforce which limits the screening process. With the advancement of sequencing technologies, the study of the entire plant genome is possible, and genetic mapping techniques such as quantitative trait locus mapping and genome-wide association studies have enabled researchers to identify loci/genes associated with various end-use quality traits in wheat. Modern breeding techniques such as marker-assisted selection and genomic selection allow the utilization of these genomic resources for the prediction of quality attributes with high accuracy and efficiency which speeds up crop improvement and cultivar development endeavors. In addition, the candidate gene approach through functional as well as comparative genomics has facilitated the translation of the genomic information from several crop species including wild relatives to wheat. This review discusses the various end-use quality traits of wheat, their genetic control mechanisms, the use of genetics and genomics approaches for their improvement, and future challenges and opportunities for wheat breeding.

show abstract

Developing Chenopodium ficifolium as a potential B genome diploid model system for genetic characterization and improvement of allotetraploid quinoa (Chenopodium quinoa)

Cited by 11 publications

References 38 publications

Genomic Sequence of Canadian Chenopodium berlandieri: A North American Wild Relative of Quinoa

Genomic Sequence of Canadian Chenopodium berlandieri: A North American Wild Relative of Quinoa

TheFLOWERING LOCUS T LIKE 2-1gene ofChenopodiumtriggers precocious flowering in Arabidopsis seedlings

Wheat end-use quality: State of art, genetics, genomics-assisted improvement, future challenges, and opportunities

Contact Info

Product

Resources

About