This article discusses the process of developing and perceptional validation by prospective users of the augmented reality application on mobile devices for three-dimensional (3D) visualization of museum collectibles. The main purpose of this publication is to visualize museum collection objects in 3D models on Android mobile devices so that visitors could be able to see collections from all sides. Augmented reality application displays a 3D model by scanning a unique marker for every collection. The test results showed that the application worked properly on smartphones using Android 4.0 or later version and with a minimum of 1 GB of memory in low-light room condition or brighter. Moreover, test results on various markers conditions indicated that the application could still be able to read the marker and display a 3D model with the marker condition that was crossed out with a ballpoint or there were traces of the folded line. In addition, if the marker was covered with a solid object such as paper with a maximum of 40%, the marker was still readable by the application. The results of the perceptual evaluation by users showed that the average of acceptance rate was 82.282% at the Nusantara Keris Museum and 88.47% at the Surakarta Cultural Heritage Museum.