Developing imprinted polymer nanoparticles for the selective separation of antidiabetic drugs

Abstract

In this study, new molecularly imprinted polymer (MIP) nanoparticles are designed for selective recognition of different drugs used for the treatment of type 2 diabetes mellitus, i.e. sitagliptin (SG) and metformin (MF). The SG- and MF-imprinted polymer nanoparticles are synthesized by free-radical initiated polymerization of the functional monomers: methacrylic acid and methyl methacrylate; and the crosslinker: ethylene glycol dimethacrylate. The surface morphology of resultant MIP nanoparticles is studied by atomic force microscopy. Fourier transform infrared spectra of MIP nanoparticles suggest the presence of reversible, non-covalent interactions between the template and the polymer. The effect of pH on the rebinding of antidiabetic drugs with SG- and MF-imprinted polymers is investigated to determine the optimal experimental conditions. The molecular recognition characteristics of SG- and MF-imprinted polymers for the respective drug targets are determined at low concentrations of SG (50-150 ppm) and MF (5-100 ppm). In both cases, the MIP nanoparticles exhibit higher binding response compared to non-imprinted polymers. Furthermore, the MIPs demonstrate high selectivity with four fold higher responses toward imprinted drugs targets, respectively. Recycled MIP nanoparticles retain 90% of their drug-binding efficiency, which makes them suitable for successive analyses with significantly preserved recognition features.

P2BM.6 - Tailoring Molecular Imprinted Polymers as Biomimetic Recognition Coatings for Anti-Diabetic Drugs

“…In previous studies [4], we showed that sitagliptin or metformin imprinted particles could be used efficiently for respective drug rebinding. The cross sensitivity of the prepared imprinted particles was substantially high for target drug rebinding.…”

A new molecularly imprinted polymer for selective extraction and pre‐concentration of guaifenesin in different samples: Adsorption studies and kinetic modeling

“…The supernatant was filtered through a 0.45 µm Millipore membrane filter and analyzed for the residual concentration of GUA. The amount of GUA adsorbed per unit mass of the adsorbent (Qe) was calculated according to Equation : where C 0 and C e are the initial and equilibrium concentrations of the GUA (mg/L), V is the solution volume (L), and m is the amount of the adsorbent (g) .…”

“…where C 0 and C e are the initial and equilibrium concentrations of the GUA (mg/L), V is the solution volume (L), and m is the amount of the adsorbent (g) [33].…”

Molecular Imprinting in Separation Science

“…A group of eight papers is mainly concerned with environmental issues, including analyses of water , food and other samples . Determination of drugs in various matrices is the third group of papers concerning mostly blood and urine. Naturally occurring substances such as protoberberines , protochatechuic acid and baicalin , mainly from plants, have been also determined.…”