Developing IMS–IMS–MS for rapid characterization of abundant proteins in human plasma

Valentine, Stephen J.; Kurulugama, Ruwan T.; Bohrer, Brian C.; Merenbloom, Samuel I.; Sowell, Renã A.; Mechref, Yehia; Clemmer, David E.

doi:10.1016/j.ijms.2009.02.030

Cited by 36 publications

(38 citation statements)

References 110 publications

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“…Numerous researchers have since demonstrated the potential of using ESI-IMS for the analysis of a variety of semi-volatile and non-volatile compounds, including environmental contaminants [8][9][10], illicit drugs [11][12][13][14][15][16][17], pharmaceuticals [18][19][20][21][22], chemical warfare agents [23][24][25], explosives [26][27][28][29] and biological molecules [30][31][32][33]. The bulk of the research has been performed on custom-built instruments in academic or government research labs.…”

Section: Introductionmentioning

confidence: 99%

Improved analysis of explosives samples with electrospray ionization-high resolution ion mobility spectrometry (ESI-HRIMS)

Hilton

Krueger

Midey

et al. 2010

International Journal of Mass Spectrometry

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Section: Introductionmentioning

confidence: 99%

Improved analysis of explosives samples with electrospray ionization-high resolution ion mobility spectrometry (ESI-HRIMS)

Hilton

Krueger

Midey

et al. 2010

International Journal of Mass Spectrometry

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“…(127) Peptide mobilities have specific signatures which can be correlated with chemical characteristics such as hydrophobicity. (128) Several groups have used IMS-MS for bottom-up proteomics analysis of biological samples such as human plasma, (104,129) mouse plasma, (112) Drosophila, (114,127,130) combinatorial peptide libraries, (131 -133) and other standard tryptic peptide mixtures. (78,104,119,134) Sowell et al (111) reported on the use of LC-IMS-MS for top-down proteomics analysis of intact proteins; however, this is an area that could be further explored.…”

Section: Proteomicsmentioning

confidence: 99%

Ion Mobility‐Mass Spectrometry

Jiang

Robinson

2013

Encyclopedia of Analytical Chemistry

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Ion mobility spectrometry (IMS) separates ions based on their mobility in an inert buffer gas in the presence of an electric field. The mobility of ions is based on their size, shape, and charge, thus IMS provides insights into structure. In addition to being used for structural information, IMS can also be used as a separation device for complex mixtures. When coupled with mass spectrometry (MS), IMS–MS offers a powerful hybrid analytical technique that has many biological, pharmaceutical, structural, environmental, and other applications. This article provides an overview of IMS–MS, which focuses on principles of drift‐time ion mobility spectrometry (DTIMS), high‐field‐asymmetric ion mobility spectrometry (FAIMS), and traveling wave ion mobility spectrometry (TWIMS) methods. Several IMS–MS instruments are discussed and examples of the current applications of the technology are provided.

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“…Recently, several studies have focused on improving the efficiency of IMS measurements by using two-dimensional mobility separations [33, 34, 41–47]. In the IMS-IMS strategy pursued in our laboratory, ion structures (which influence the ions’ mobilities) are altered between the two separation steps.…”

Section: Introductionmentioning

confidence: 99%

Complexation of Amino Compounds by 18C6 Improves Selectivity by IMS-IMS-MS: Application to Petroleum Characterization

Valentine

Clemmer

2011

J. Am. Soc. Mass Spectrom.

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Complexation of a series of related amino compounds by 18-crown-6 ether (18C6) is studied as a means of improving the resolution of mixtures by combinations of ion mobility spectrometry (IMS) and mass spectrometry (MS) techniques. Mixtures of the isomeric amines n-octylamine (NOA), dibutylamine (DBA), and diisopropylethylamine (DIPEA) were electrosprayed to produce gaseous [M + H]+ ions. These species have overlapping mobilities and are not resolved by IMS. Addition of 18C6 yields [M + 18C6 + H]+ ion complexes that are resolved by IMS. In subsequent experiments, [M + 18C6 + H]+ ion complexes are separated according to their mobilities and specific species are selected and exposed to collisional activation. This analysis yields dissociation voltages that are inversely correlated with the number of separate substitutions on the nitrogen atom of the amino compounds; dissociation voltages of ~40, ~90, and ~150 V are obtained for the tri-, di-, and mono-substituted amino compounds DIPEA, DBA, and NOA, respectively. For these complexes, an inverse correlation is also observed with respect to the gas-phase basicities (GB) of the amino compounds (964, 935, and 895 kJ mol−1, respectively). Studies of 18C6 complexes with a series of n-alkylamines (CnH2n+3N where n=3 to 18, respectively) show that dissociation voltages increase systematically (from ~140 to ~190 V) under the conditions employed. The sensitivity to collision energy provides an additional means of distinguishing between classes of compounds. The approach is extended as a means of separating nitrogen-containing compounds from petroleum.

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Developing IMS–IMS–MS for rapid characterization of abundant proteins in human plasma

Cited by 36 publications

References 110 publications

Improved analysis of explosives samples with electrospray ionization-high resolution ion mobility spectrometry (ESI-HRIMS)

Improved analysis of explosives samples with electrospray ionization-high resolution ion mobility spectrometry (ESI-HRIMS)

Ion Mobility‐Mass Spectrometry

Complexation of Amino Compounds by 18C6 Improves Selectivity by IMS-IMS-MS: Application to Petroleum Characterization

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