Developing multifunctional crops by engineering Brassicaceae glucosinolate pathways

Qin, Han; King, Graham J.; Borpatragohain, Priyakshee; Zou, Jun

doi:10.1016/j.xplc.2023.100565

Cited by 16 publications

(8 citation statements)

References 294 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since Met and Trp are precursors of aliphatic and indole glucosinolates, respectively [ 36 ] ( Figure 2 A,B), in order to determine whether exogenous Met and Trp can promote the biosynthesis of aliphatic and indole glucosinolates, the contents of aliphatic and indole glucosinolates in broccoli seedlings that were treated with Trp, Met, or both was determined. Compared to the control, the contents of aliphatic glucosinolates (glucoiberin, glucoraphanin, glucoalyssin, and glucoerucin) in broccoli seedlings that were treated with Met significantly increased ( Figure 2 C), with the content of glucoraphanin, a precursor of the anticancer compound SFN, increasing to 2.37 times that of the untreated group.…”

Section: Resultsmentioning

confidence: 99%

Application of Tryptophan and Methionine in Broccoli Seedlings Enhances Formation of Anticancer Compounds Sulforaphane and Indole-3-Carbinol and Promotes Growth

Li,

Zhou,

Zhao

et al. 2024

Foods

View full text Add to dashboard Cite

Broccoli is a popular cruciferous vegetable that is well known for its abundant health-promoting biochemicals. The most important of these beneficial biochemicals are glucosinolates, including glucoraphanin and glucobrassicin. Glucoraphanin and glucobrassicin can be broken down by myrosinases into sulforaphane and indole-3-carbinol, which have been demonstrated to have potent cancer-preventive properties. Efforts to increase glucoraphanin in broccoli seedlings have long been a focus; however, increasing glucoraphanin and glucobrassicin simultaneously, as well as enhancing myrosinase activity to release more sulforaphane and indole-3-carbinol, have yet to be investigated. This study aims to investigate the impact of the combined application of tryptophan and methionine on the accumulation of sulforaphane and indole-3-carbinol, as well as their precursors. Furthermore, we also examined whether this application has any effects on seedling growth and the presence of other beneficial compounds. We found that the application of methionine and tryptophan not only increased the glucoraphanin content by 2.37 times and the glucobrassicin content by 3.01 times, but that it also caused a higher myrosinase activity, resulting in a1.99 times increase in sulforaphane and a 3.05 times increase in indole-3-carbinol. In addition, better plant growth and an increase in amino acids and flavonoids were observed in broccoli seedlings with this application. In conclusion, the simultaneous application of tryptophan and methionine to broccoli seedlings can effectively enhance their health-promoting value and growth. Our study provides a cost-effective and multi-benefit strategy for improving the health value and yield of broccoli seedlings, benefiting both consumers and farmers.

show abstract

Section: Resultsmentioning

confidence: 99%

Application of Tryptophan and Methionine in Broccoli Seedlings Enhances Formation of Anticancer Compounds Sulforaphane and Indole-3-Carbinol and Promotes Growth

Li,

Zhou,

Zhao

et al. 2024

Foods

View full text Add to dashboard Cite

show abstract

“…The glucosinolate biosynthesis, nitrogen metabolism and sulfur relay system were enriched. Glucosinolates (GSLs), found mainly in species of the Brassicaceae family, are one of the most well-studied classes of secondary metabolites and GSLs were sulfur-and nitrogen-containing secondary metabolites functioning in plant defense [37]. Interestingly, S-containing compounds like cysteine (Cys), methionine (Met) and Glutathione (GSH) were all enriched, suggested that Sulfur metabolism in seeds may has immense importance in the role of exogenous ABA during the time of seed germination [38].…”

Section: Discussionmentioning

confidence: 99%

Metabolome and transcriptome analyses reveal changes of rapeseed in response to ABA signal during early seedling development

Chen,

Wu,

et al. 2024

BMC Plant Biol

View full text Add to dashboard Cite

Seed germination is an important development process in plant growth. The phytohormone abscisic acid (ABA) plays a critical role during seed germination. However, the mechanism of rapeseed in response to ABA is still elusive. In order to understand changes of rapeseed under exogenous ABA treatment, we explored differentially expressed metabolites (DEMs) and the differentially expressed genes (DEGs) between mock- and ABA-treated seedlings. A widely targeted LC-MS/MS based metabolomics were used to identify and quantify metabolic changes in response to ABA during seed germination, and a total of 186 significantly DEMs were identified. There are many compounds which are involved in ABA stimuli, especially some specific ABA transportation-related metabolites such as starches and lipids were screened out. Meanwhile, a total of 4440 significantly DEGs were identified by transcriptomic analyses. There was a significant enrichment of DEGs related to phenylpropanoid and cell wall organization. It suggests that exogenous ABA mainly affects seed germination by regulating cell wall loosening. Finally, the correlation analysis of the key DEMs and DEGs indicates that many DEGs play a direct or indirect regulatory role in DEMs metabolism. The integrative analysis between DEGs and DEMs suggests that the starch and sucrose pathways were the key pathway in ABA responses. The two metabolites from starch and sucrose pathways, levan and cellobiose, both were found significantly down-regulated in ABA-treated seedlings. These comprehensive metabolic and transcript analyses provide useful information for the subsequent post-transcriptional modification and post germination growth of rapeseed in response to ABA signals and stresses.

show abstract

“…GSLs are a diverse group of nitrogen and sulpfur-containing SM found in the Brassicaceae family plants including Arabidopsis 111,127 . GSLs play important roles in plant defense against various pests and diseases and have significant medicinal values 127,128 . They can be converted by intestinal bacteria into isothiocyanates, which have anti-inflammatory, anti-cancer, antiobesity, and neuroprotective properties 129 .…”

Section: Regulation Of Carotenoid and Artemisinin Biosynthesis By Lightmentioning

confidence: 99%

Light regulation of the biosynthesis of phenolics, terpenoids, and alkaloids in plants

Liu,

Singh,

Pattanaik

et al. 2023

Commun Biol

View full text Add to dashboard Cite

Biosynthesis of specialized metabolites (SM), including phenolics, terpenoids, and alkaloids, is stimulated by many environmental factors including light. In recent years, significant progress has been made in understanding the regulatory mechanisms involved in light-stimulated SM biosynthesis at the transcriptional, posttranscriptional, and posttranslational levels of regulation. While several excellent recent reviews have primarily focused on the impacts of general environmental factors, including light, on biosynthesis of an individual class of SM, here we highlight the regulation of three major SM biosynthesis pathways by light-responsive gene expression, microRNA regulation, and posttranslational modification of regulatory proteins. In addition, we present our future perspectives on this topic.

show abstract

Developing multifunctional crops by engineering Brassicaceae glucosinolate pathways

Cited by 16 publications

References 294 publications

Application of Tryptophan and Methionine in Broccoli Seedlings Enhances Formation of Anticancer Compounds Sulforaphane and Indole-3-Carbinol and Promotes Growth

Application of Tryptophan and Methionine in Broccoli Seedlings Enhances Formation of Anticancer Compounds Sulforaphane and Indole-3-Carbinol and Promotes Growth

Metabolome and transcriptome analyses reveal changes of rapeseed in response to ABA signal during early seedling development

Light regulation of the biosynthesis of phenolics, terpenoids, and alkaloids in plants

Contact Info

Product

Resources

About