Developing partial interspecific hybrids of Momordica charantia × Momordica balsamina and their advance generations

Rathod, Vijayakumar; Behera, Tusar Kanti; Munshi, A. D.; Gaikwad, Ambika B.; Singh, S. K.; Vinay, N.D.; Boopalakrishnan, G.; Jat, Gograj Singh

doi:10.1016/j.scienta.2021.109985

Cited by 11 publications

(10 citation statements)

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“…These accessions showed a wide morphological variation with regard to a number of traits, including flowering time, fruit shape, size, color, and yield variation. In the bitter gourd germplasm, maximum diversity was observed for numerous characteristics, including sex expression (i.e., gynoecious, predominantly gynoecious, or monoecious [8]), growth habit, maturity, fruit shape, size, color and surface texture [9,10,13,14,26]. Line DBGS-38-1 recorded the highest number of fruits per plant, which could be due to the presence of the maximum numbers of female and male flowers (desirable ratio of female to male flowers) during the whole growing season.…”

Section: Discussionmentioning

confidence: 99%

“…The primary center of origin for bitter gourd is probably India, with China as a secondary center of diversity [6,7]. India is richly endowed with bitter gourd genetic resources, as Indian bitter gourd germplasms are diverse in phenotypic characteristics, i.e., sex expression [8], growth habit, maturity, fruit shape, size, color and surface texture [9][10][11][12][13][14]. Nevertheless, Indian bitter gourd accessions exhibit valuable genes that are as yet unexplored [15].…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Genetic Diversity and Population Structure in Bitter Gourd (Momordica charantia L.) Using Morphological and SSR Markers

Alhariri

Behera

Jat

et al. 2021

Plants

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The present investigation was carried out using 51 diverse bitter gourd accessions as material for studying genetic diversity and relatedness using morphological and SSR markers. A wide variation was observed for morphological traits like the number of days to the first female flower anthesis (37.33–60.67), the number of days to the first fruit harvest (47.67–72.00), the number of fruits/plant (12.00–46.67), fruit length (5.00–22.23 cm), fruit diameter (1.05–6.38 cm), average fruit weight (20.71–77.67 g) and yield per plant (513.3–1976 g). Cluster analysis for 10 quantitative traits grouped the 51 accessions into 6 clusters. Out of 61 SSR primers screened, 30 were polymorphic and highly informative as a means to differentiate these accessions. Based on genotyping, a high level of genetic diversity was observed, with a total of 99 alleles. The polymorphic information content (PIC) values ranged from 0.038 for marker BG_SSR-8 to 0.721 for S-24, with an average of 0.429. The numbers of alleles ranged from 2 to 5, with an average of 3.3 alleles per locus. Gene diversity ranged from 0.04 for BG_SSR-8 to 0.76 for S-24, showing a wide variation among 51 accessions. The UPGMA cluster analysis grouped these accessions into 3 major clusters. Cluster I comprised 4 small, fruited accessions that are commercially cultivated in central and eastern India. Cluster II comprised 35 medium- to long-sized fruited accessions, which made up an abundant and diverse group. Cluster III comprised 11 long and extra-long fruited accessions. The polymorphic SSR markers of the study will be highly useful in genetic fingerprinting and mapping, and for association analysis in Momordica regarding several economic traits.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analysis of Genetic Diversity and Population Structure in Bitter Gourd (Momordica charantia L.) Using Morphological and SSR Markers

Alhariri

Behera

Jat

et al. 2021

Plants

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“…did not manifest the expected ratios of a true F1 hybrid, and in all cross configurations the progeny were non-Mendelian partial hybrids, with the female parent predominating 32 . In interspecific hybrids of Momordica charantia L. × M. balsamina L., SSR analysis showed that amplified fragments of interspecific hybrids exhibited highly conserved similarity with alleles of the female parents, with a very low frequency of male fragments Rathod et al 33 .…”

Section: Discussionmentioning

confidence: 99%

“…In interspecific hybrids of Momordica charantia L. × Momordica balsamina L., SSR analysis showed that amplified fragments of interspecific hybrids exhibited highly conserved similarity with alleles of the female parents, with a very low frequency of male fragments Rathod et al . [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of intergeneric hybrids between Malus and Pyrus

Pasqualetto

Palmieri

Martens

et al. 2022

Horticulture Research

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Apple (Malus) and pear (Pyrus) are economically important fruit crops well known for their unique textures, flavours, and nutritional qualities. Both genera are characterised by a distinct pattern of secondary metabolites, which directly affect not only resistance to certain diseases, but also have significant impacts on the flavour and nutritional value of the fruit. The identical chromosome numbers, similar genome size, and their recent divergence date, together with DNA markers have shown that apple and pear genomes are highly co-linear. This study utilized comparative genomic approaches, including simple sequence repeats, high resolution single nucleotide polymorphism melting analysis, and single nucleotide polymorphism chip analysis to identify genetic differences among hybrids of Malus and Pyrus, and F2 offspring. This research has demonstrated and validated that these three marker types, along with metabolomics analysis are very powerful tools to detect and confirm hybridity of progeny derived from crosses between apple and pear in both cross directions. Furthermore, this work analysed the genus-specific metabolite patterns and the resistance to fire blight (Erwinia amylovora) in progeny. The findings of this work will enhance and accelerate the breeding of novel tree fruit crops that benefit producers and consumers, by enabling marker assisted selection of desired traits introgressed between pear and apple.

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“…M. balsamina , in particular, is considered the closest wild relative that can be crossed with Bitter gourd, falling under the II subclass of the primary gene pool of Bitter gourd ( Bharathi et al., 2012 ). M. balsamina also possesses a high level of tolerance to like pests such as ladybird beetle ( Epilacna septima ), pumpkin caterpillar ( Margaronia indica ), red pumpkin beetle ( Aulocophora fevicoli ), gall fly ( Lasioptera falcata ), root-knot nematode ( Meladogyne incognita ), and diseases such as yellow mosaic and little leaf disease, making it an invaluable genetic resource for the improvement of M. charantia ( Rathod et al., 2021 ). Hence, in addition to medicinal attributes, M. balsamina can serve as a potent genetic source of biotic stress resistance.…”

Section: Introductionmentioning

confidence: 99%

High-quality Momordica balsamina genome elucidates its potential use in improving stress resilience and therapeutic properties of bitter gourd

Vinay,

Singh,

Ellur

et al. 2024

Front. Plant Sci.

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IntroductionMomordica balsamina is the closest wild species that can be crossed with an important fruit vegetable crop, Momordica charantia, has immense medicinal value, and placed under II subclass of primary gene pool of bitter gourd. M. balsamina is tolerant to major biotic and abiotic stresses. Genome characterization of Momordica balsamina as a wild relative of bitter gourd will contribute to the knowledge of the gene pool available for improvement in bitter gourd. There is potential to transfer gene/s related to biotic resistance and medicinal importance from M. balsamina to M. charantia to produce high-quality, better yielding and stress tolerant bitter gourd genotypes.MethodsThe present study provides the first and high-quality chromosome-level genome assembly of M. balsamina with size 384.90 Mb and N50 30.96 Mb using sequence data from 10x Genomics, Nanopore, and Hi-C platforms.ResultsA total of 6,32,098 transposons elements; 2,15,379 simple sequence repeats; 5,67,483 transcription factor binding sites; 3,376 noncoding RNA genes; and 41,652 protein-coding genes were identified, and 4,347 disease resistance, 67 heat stress–related, 05 carotenoid-related, 15 salt stress–related, 229 cucurbitacin-related, 19 terpenes-related, 37 antioxidant activity, and 06 sex determination–related genes were characterized.ConclusionGenome sequencing of M. balsamina will facilitate interspecific introgression of desirable traits. This information is cataloged in the form of webgenomic resource available at http://webtom.cabgrid.res.in/mbger/. Our finding of comparative genome analysis will be useful to get insights into the patterns and processes associated with genome evolution and to uncover functional regions of cucurbit genomes.

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Developing partial interspecific hybrids of Momordica charantia × Momordica balsamina and their advance generations

Cited by 11 publications

References 38 publications

Analysis of Genetic Diversity and Population Structure in Bitter Gourd (Momordica charantia L.) Using Morphological and SSR Markers

Analysis of Genetic Diversity and Population Structure in Bitter Gourd (Momordica charantia L.) Using Morphological and SSR Markers

Molecular characterization of intergeneric hybrids between Malus and Pyrus

High-quality Momordica balsamina genome elucidates its potential use in improving stress resilience and therapeutic properties of bitter gourd

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