Developing standards and test protocols for testing the hemocompatibility of biomaterials

Braune, S.; Lendlein, Andreas; Jung, F.

doi:10.1016/b978-0-08-100497-5.00004-5

Cited by 14 publications

(7 citation statements)

References 159 publications

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“…The major prerequisite for materials intended for life science applications is their adequate biocompatibility. − Therefore, LbL films of iFPs as potential coatings for cardiovascular stents or catheters were evaluated using the widely accepted hemolysis test for evaluation of hemocompatibility of biomaterials. , It has been demonstrated that the superb hemocompatibility of clinically validated PTFEP ,, is correlated with its ability to selectively and irreversibly adsorb human serum albumin (HSA) from blood plasma . For that reason, the ability of fluorinated LbL nanofilms to bind HSA was also evaluated and compared to films of this nonionic polyphosphazene, PTFEP, which was solution-cast from ethyl acetate.…”

Section: Resultsmentioning

confidence: 99%

Biocompatible Nanocoatings of Fluorinated Polyphosphazenes through Aqueous Assembly

Selin

Albright

Ankner

et al. 2018

ACS Appl. Mater. Interfaces

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Nonionic fluorinated polyphosphazenes, such as poly[bis(trifluoroethoxy)phosphazene] (PTFEP), display superb biocompatibility, yet their deposition to surfaces has been limited to solution casting from organic solvents or thermal molding. Herein, hydrophobic coatings of fluorinated polyphosphazenes are demonstrated through controlled deposition of ionic fluorinated polyphosphazenes (iFPs) from aqueous solutions using the layer-by-layer (LbL) technique. Specifically, the assemblies included poly[(carboxylatophenoxy)(trifluoroethoxy)phosphazenes] with varied content of fluorine atoms as iFPs (or poly[bis(carboxyphenoxy)phosphazene] (PCPP) as a control nonfluorinated polyphosphazene) and a variety of polycations. Hydrophobic interactions largely contributed to the formation of LbL films of iFPs with polycations, leading to linear growth and extremely low water uptake. Hydrophobicity-enhanced ionic pairing within iFP/BPEI assemblies gave rise to large-amplitude oscillations in surface wettability as a function of capping layer, which were the largest for the most fluorinated iFP, while control PCPP/polycation systems remained hydrophilic regardless of the film top layer. Neutron reflectometry (NR) studies indicated superior layering and persistence of such layering in salt solution for iFP/BPEI films as compared to control PCPP/polycation systems. Hydrophobicity of iFP-capped LbL coatings could be further enhanced by using a highly porous polyester surgical felt rather than planar substrates for film deposition. Importantly, iFP/polycation coatings displayed biocompatibility which was similar to or superior to that of solution-cast coatings of a clinically validated material (PTFEP), as demonstrated by the hemolysis of the whole blood and protein adsorption studies.

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Section: Resultsmentioning

confidence: 99%

Biocompatible Nanocoatings of Fluorinated Polyphosphazenes through Aqueous Assembly

Selin

Albright

Ankner

et al. 2018

ACS Appl. Mater. Interfaces

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“…Adequate biocompatibility is one of the main requirements for materials designed for life sciences applications. − Therefore, PPz coatings were evaluated for hemolytic activity using dilute whole rabbit blood test (American Society for Testing and Materials). , Figure B presents the results of this test for coatings with matched thicknesses and PPzs as the top layer. Remarkably, both sulfonated PPzs with fluorinated side groups (FESP and FPSP) showed levels of hemolysis less than 1%.…”

Section: Results and Discussionmentioning

confidence: 99%

New Family of Water-Soluble Sulfo–Fluoro Polyphosphazenes and Their Assembly within Hemocompatible Nanocoatings

Albright

Marin

Kaner

et al. 2019

ACS Appl. Bio Mater.

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In this work, novel sulfo−fluoro polyphosphazenes (PPzs) were synthesized via macromolecular substitution of polydichlorophosphazene utilizing "non-covalent protection" methodology by converting acid functionalities into hydrophobic alkylammonium salts. Resulting PPzs showed excellent solubility in aqueous solutions over a broad pH range and contained ∼25% sulfo-groups and 20% either trifluoroethoxy-(FESP) or trifluoromethylphenoxy-(FPSP) side groups, as determined by NMR spectroscopy. Their polyelectrolyte behavior was evaluated by binding with an oppositely charged polyion, branched polyethylenimine (PEI), which resulted in the formation of interpolymer complexes as shown by dynamic light scattering (DLS). Contrary to a sulfonated, nonfluorinated PPz homopolymer (SP), fluorinated macromolecules effectively bound human serum albumin (HSA) as revealed by dynamic light scattering and asymmetric flow field flow fractionation (AF4) studies. Moreover, FESP and FPSP both displayed low hemolytic activity as evaluated in solution using porcine red blood cells. Using the layer-by-layer (LbL) technique, FESP and FPSP were assembled into nanocoatings with PEI. Both fluorinated and nonfluorinated sulfo PPzs showed linear growth with PEI because of strong ionic pairing between sulfo and amino groups. However, films of fluorinated PPzs displayed higher hydrophobicity, lower swelling, and improved stability in high ionic strength environment when compared to coatings formed by a sulfonated, nonfluorinated SP, or a carbon-chain polymer poly(styrene sulfonic acid). Hemocompatibility of FESP and FPSP nanofilms was demonstrated in vitro using whole rabbit blood hemolysis tests, which showed less than 1% hemolysis. Altogether, the present study introduces a new class of hemocompatible, sulfo−fluoropolymers that shows promise for life science applications.

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“…47,48 Macrophages secrete high levels of proinflammatory reactive oxygen species (ROS) that enhance inflammatory responses and cellular damage. 49 Furthermore, inflammation and coagulation promoted each other, and excessive inflammation induced coagulation and increased the risk of infection. 50 To study the response of monocytes on the coatings, we incubated the samples with mouse macrophage leukemia cells (RAW 264.7).…”

Section: Anticoagulant Performance Of the Hydrogelmentioning

confidence: 99%

Hemocompatibility Multi-in-One Hydrogel Coating with ROS-Triggered Inflammation Suppression and Anti-Infection Properties for Blood-Contacting Device

Wei

Liu

et al. 2022

Biomacromolecules

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In traditional blood-contacting medical devices, infection and thrombosis are easily formed on the surface of the materials. In addition, inflammation is also a clinical complication that cannot be ignored. More importantly, there is a mutually promoting relationship between the inflammatory response and the infection as well as thrombosis. In this work, we propose a self-adaptive antiinflammatory coating strategy combined with anti-infection and anticoagulant capacity, which was accomplished based on nano-Ag particles and dexamethasone (Dex)-loaded hydrogel coating. The coating loaded with nano-Ag endows it with good bactericidal performance, including Gram-positive and Gram-negative bacteria. As an anti-inflammatory drug, Dex was grafted onto hydrogel coating by a reactive oxygen species (ROS)-cleavable thioketal (TK) bond and released upon the trigger of an inflammatory environment, blocking further inflammatory cascade, providing self-adaptive antiinflammatory properties, and avoiding side effects of the drug. It was demonstrated that the coating worked as a precise strategy to resist coagulation, infection, and inflammation, provided a new perspective for designing clinical complication-conformable coatings, and had great application prospects on blood-contacting medical devices.

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Developing standards and test protocols for testing the hemocompatibility of biomaterials

Cited by 14 publications

References 159 publications

Biocompatible Nanocoatings of Fluorinated Polyphosphazenes through Aqueous Assembly

Biocompatible Nanocoatings of Fluorinated Polyphosphazenes through Aqueous Assembly

New Family of Water-Soluble Sulfo–Fluoro Polyphosphazenes and Their Assembly within Hemocompatible Nanocoatings

Hemocompatibility Multi-in-One Hydrogel Coating with ROS-Triggered Inflammation Suppression and Anti-Infection Properties for Blood-Contacting Device

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