Development and analysis of an integral fluidodynamic model in hollow fibre for different operational modes

Camacho, F.; Jurado, E.; Luzón, G.; Vicaria, José M.

doi:10.1016/j.memsci.2009.10.013

Cited by 3 publications

(2 citation statements)

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“…Several initial assumptions normally considered by different researchers were assumed: isothermal process; Newtonian uncompressible fluid with constant physical properties; flow rates and pressures being uniformly distributed over the module cross-section; fiber bundles being regarded as a collection of parallel rods, which means that the packing-density distribution and flow distribution were consistent along the module length; the fibers had the same diameter, were rigid and could not be deformed; and entrance and exit effects in the lumen as well as the end effects in the shell were ignored 28 .…”

Section: Assumptions Of the Modelmentioning

confidence: 99%

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Diffusion and Inhibition Processes in a Hollow-fiber Membrane Bioreactor for Hybridoma Culture. Development of a Mathematical Model

Legazpi

Laca

Collado

et al. 2016

Chem.Biochem.Eng.Q.

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The performance of a hollow-fiber membrane bioreactor (HFBR) (molecular weight cut-off 30 kD, fiber surface area 2050 cm 2 ) containing a culture of hybridoma cells has been investigated. Experimental data were used as basis to develop a model of general application. Concentrations of fundamental nutrients (glucose and glutamine), inhibitory products (ammonium and lactate), and monoclonal antibodies (MAb) against bovine lactoferrin (IgG 1 ) were monitored over time. Exchange of nutrients and products occurred across the capillary surface, whereas cells and MAb remained in the extra-capillary space (ECS). A protein-free culture medium (Hybrimax) with and without antibiotics was used. In both cases, the final MAb concentration was the same; however, antibiotic presence slowed down the time to achieve this concentration. Diffusion assays have been carried out in order to support the development of a mathematical model that describes the performance of the HFBR, including mass transfer and reaction terms. Inhibition by ammonium and lactate has been considered in the kinetics, providing model results consistent with experimental data. Further research with other cell lines and/or culture media will allow to broaden the field of application of this model for general use in HFBR systems.

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Section: Assumptions Of the Modelmentioning

confidence: 99%

“…Likewise, the flow resistance and the possible change in membrane properties caused by the adhesion of cells to the fiber membranes were also supposed negligible 9,28 . The material that disappears/appears in the bottle is equal to the material that passes through the fiber walls:…”

Section: Model Equationsmentioning

confidence: 99%