Development and application of a liquid chromatographic method for analysis of nucleotides and nucleosides in milk and infant formulas

Gill, Brendon D.; Indyk, Harvey E.

doi:10.1016/j.idairyj.2006.08.001

Cited by 115 publications

(55 citation statements)

References 38 publications

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“…Initially, the concentration of nucleotides in colostrum is very low, but reaches a maximum 24-48 h after parturition, followed by a gradual decrease as lactation continues, up to the third week, when levels stabilise (Gill and Indyk, 2007a). This pattern is seen for nucleoside-5′-monophosphates; 5′-AMP, 5′CMP and 5′UMP, as well as nucleoside-5′-, di-and tri-phosphates, UDP galactose and UDP glucose (Gill and Sanchez-Medina 1981).…”

Section: Nucleotides and Nucleosidesmentioning

confidence: 99%

Composition and properties of bovine colostrum: a review

McGrath

Fox

McSweeney

et al. 2015

Dairy Sci. & Technol.

314

263

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Colostrum is the initial milk secreted by mammals following parturition, the composition and physicochemical properties of which are highly dynamic and variable. The composition and physicochemical properties of colostrum during the initial postpartum period has not been systematically reviewed for many years, although the topic remains of interest both to milk producers and processors. In this article, the current understanding of the composition of colostrum, i.e. carbohydrates, proteins, growth factors, enzymes, enzyme inhibitors, nucleotides and nucleosides, cytokines, fats, vitamins and minerals, is reviewed. In addition, the physicochemical properties, i.e. pH and buffering capacity, colour, density and specific gravity, osmotic pressure, somatic cell count, properties of casein micelles, ethanol stability and rennet coagulation properties are discussed, as well as the effects of heat-treating colostrum.

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Section: Nucleotides and Nucleosidesmentioning

confidence: 99%

Composition and properties of bovine colostrum: a review

McGrath

Fox

McSweeney

et al. 2015

Dairy Sci. & Technol.

314

263

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“…stress, illness and the gastrointestinal tract disorders. Colostrum increases cell proliferation and migration, enhances gut immunity, and therefore can be used to treat or prevent infections of the gastrointestinal tract (Antonio et al 2001;Gill and Indyk 2007;Gill et al 2012). …”

Section: Goat's Colostrum Composition Vitamin a Vitamin Ementioning

confidence: 99%

Dynamical changes of basic chemical indicators and significant lipophilic vitamins in caprine colostrum

2014

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Colostrum is currently used as a food supplement. It has been sought after by an increasing number of consumers for its unique composition. Its use is supported by EU legislation that defines colostrum as an animal product for human consumption. Therefore, information on its chemical properties and the content of lipophilic vitamins are important when evaluating colostrum quality and safety. The aim of this study was to assess the dynamic changes of basic chemical properties (protein, fat, lactose, and dry matter) and the content of selected lipophilic vitamins (vitamin A and vitamin E) in white short-haired goat's colostrum. Attention was paid to the first milk from the first hour to the sixth day after parturition as the initial milk composition changes rapidly during this period. Protein, fat, and lactose concentrations were measured by near infrared spectroscopy, vitamins were analysed simultaneously by ultra-high performance liquid chromatography with detection in the ultraviolet part of the spectrum. The most significant changes in chemical indicators occurred during the first three days (P ≤ 0.01) post partum. The highest decrease was observed in protein concentrations. It was × 2 lower than in the first hour post partum. The concentration of vitamin A rapidly decreased during 36 h. In the following hours, gradual changes were observed (P > 0.05). The analysed components in caprine colostrum were similar to mature milk after 132 h post partum. Goat's colostrum, composition, vitamin A, vitamin EColostrum is the initial mammary secretion after parturition. It is secreted for approximately first 5-7 days after delivery. Considering its unique composition and current legislation, colostrum has attracted a great deal of attention in the pharmaceutical industry and has been used as a food supplement for promoting health and enhancing the immune system. Colostrum is an excellent supplement recommended especially for the elderly, sportsmen and in some specific conditions, e.g. stress, illness and the gastrointestinal tract disorders. Colostrum increases cell proliferation and migration, enhances gut immunity, and therefore can be used to treat or prevent infections of the gastrointestinal tract (Antonio et al. 2001;Gill and Indyk 2007;Gill et al. 2012).This first mammary secretion is considered as an important nutritional supplement. It contains more dry matter, mineral salts, and proteins but less lactose than mature milk. Furthermore, the fat content is often, but not always, higher than that of milk. The proportion of whey proteins is substantially higher in colostrum than in milk. The foremost protein fraction in colostrum consists of immunoglobulines (IgG, IgM and IgA). Changes in the composition of fatty acids are mostly qualitative; colostrum usually contains more polyunsaturated fatty acids, phospholipids and cholesterol than mature milk (Roginski et al. 2002;Uruakpa et al. 2002;Fox and Kelly 2006).Concentrations of fat soluble vitamins A, D, E, and carotenoids and water soluble vitamins B 1 , B 2...

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“…A number of analytical procedures have been proposed for the determination of nucleobases including electrochemical techniques (Ba et al 2012;Wei et al 2011;Huang et al 2011;Li et al 2013;Svorc and Kalchar 2014), high-performance liquid chromatography (Gill and Indyk 2007;Yeung et al 2008;Graven et al 2014), capillary electrophoresis (Ye and Jiang 2002;RodriguezGomez and Carabias-Martinez 2014;Haunschmidt et al 2008), spectroscopic methods (Amri et al 2003;Heisler et al 2002), chemiluminescence (Liu and Xue 2006), and mass spectrometry (Huang and Chang 2007). Capillary gas chromatography (GC) has high potential to separate closely related organic compounds in a short time.…”

Section: Introductionmentioning

confidence: 99%