Development and application of a novel multiplex PCR assay for the differentiation of four haemosporidian parasites in the chicken Gallus gallus domesticus

Xuan, Mai Nguyen Thi; Kaewlamun, Winai; Saiwichai, Tawee; Thanee, Suchansa; Poofery, Juthathip; Tiawsirisup, Sonthaya; Channumsin, Manun; Kaewthamasorn, Morakot

doi:10.1016/j.vetpar.2021.109431

Cited by 10 publications

(5 citation statements)

References 29 publications

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“…The application of CISH tools using parasite-specific probes opens new opportunities to link tissue stages found in wild birds with certain haemosporidian parasites [ 93 , 94 , 95 , 234 , 250 , 251 , 252 ]. This was difficult or impossible to do before the molecular era because co-infections of Apicomplexan parasites are common and even predominating in many avian hosts worldwide [ 4 , 7 , 9 , 10 , 23 , 38 , 40 , 42 , 58 , 105 , 110 , 118 , 253 , 254 , 255 , 256 , 257 , 258 , 259 , 260 , 261 , 262 , 263 , 264 , 265 , 266 , 267 , 268 , 269 , 270 , 271 , 272 , 273 , 274 ]. Recent molecular and microscopic studies show that the morphological diversity of haemosporidian exo-erythrocytic stages is unexpectedly diverse, particularly in Haemoproteus and Leucocytozoon parasites, which have historically been difficult or impossible to link to certain parasite species or lineages, even on genera levels, due to common co-infections […”

Section: Resultsmentioning

confidence: 99%

Insights into the Biology of Leucocytozoon Species (Haemosporida, Leucocytozoidae): Why Is There Slow Research Progress on Agents of Leucocytozoonosis?

Valkiūnas

Iezhova

2023

Microorganisms

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Blood parasites of the genus Leucocytozoon (Leucocytozoidae) only inhabit birds and represent a readily distinct evolutionary branch of the haemosporidians (Haemosporida, Apicomplexa). Some species cause pathology and even severe leucocytozoonosis in avian hosts, including poultry. The diversity of Leucocytozoon pathogens is remarkable, with over 1400 genetic lineages detected, most of which, however, have not been identified to the species level. At most, approximately 45 morphologically distinct species of Leucocytozoon have been described, but only a few have associated molecular data. This is unfortunate because basic information about named and morphologically recognized Leucocytozoon species is essential for a better understanding of phylogenetically closely related leucocytozoids that are known only by DNA sequence. Despite much research on haemosporidian parasites during the past 30 years, there has not been much progress in taxonomy, vectors, patterns of transmission, pathogenicity, and other aspects of the biology of these cosmopolitan bird pathogens. This study reviewed the available basic information on avian Leucocytozoon species, with particular attention to some obstacles that prevent progress to better understanding the biology of leucocytozoids. Major gaps in current Leucocytozoon species research are discussed, and possible approaches are suggested to resolve some issues that have limited practical parasitological studies of these pathogens.

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Section: Resultsmentioning

confidence: 99%

Insights into the Biology of Leucocytozoon Species (Haemosporida, Leucocytozoidae): Why Is There Slow Research Progress on Agents of Leucocytozoonosis?

Valkiūnas

Iezhova

2023

Microorganisms

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“…The global prevalence of haemosporidia in red junglefowl was 74.8% (175/234), which is much higher than that of fighting cocks from Thailand (20.8%, 52/250) [ 15 ], but lower than that in domestic chickens from Nan, Prachinburi, and Chachoengsao provinces of Thailand (79.6%, 125/157) [ 16 ] and in indigenous chickens from the north central part of Nigeria (75.0%, 81/108) [ 17 ]. The reason for this may be the abundance of vegetation in tropical areas, with species of Culicoides and avian haemosporidia transmitted by biting midges and other insect vectors [ 18 , 19 ].…”

Section: Discussionmentioning

confidence: 99%

First report of haemosporidia and associated risk factors in red junglefowl (Gallus gallus) in China

et al. 2022

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Background Avian haemosporidia infect both domestic and wild birds, causing anemia, acute tissue degeneration, and depopulation in wild birds. Poultry and wild birds have been reported as common reservoirs of haemosporidia, but limited information is available for red junglefowl (Gallus gallus) in China. The present study investigated the prevalence and molecular characterization of haemosporidia in red junglefowl. Methods Blood samples were collected from 234 red junglefowl from Jinghong City of Yunnan Province, and genomic DNA was extracted from these samples. The prevalence of haemosporidia was determined by nested PCR targeting the mitochondrial cytochrome b (cytb) gene. Molecular characterization was investigated based on phylogenetic analysis of cytb sequences, and associated risk factors were analyzed using the Chi-square (χ2) test. Results The overall prevalence of haemosporidia was 74.8% (175/234), and three species were identified, namely Haemoproteus enucleator, Leucocytozoon californicus, and Plasmodium juxtanucleare. The prevalence of haemosporidia in adult fowl (81.1%, 107/132) was significantly higher (χ2 = 6.32, df = 1, P = 0.012) than that in juveniles (66.7%, 68/102). Three novel haemosporidian lineages were revealed. Conclusions This study examined the prevalence and identified species of avian haemosporidians in red junglefowl, providing new information on the molecular epidemiology and geographical distribution of haemosporidian parasites. Our results indicated high prevalence and diverse species distribution of these haemosporidians in red junglefowl. To the best of our knowledge, this is the first record of haemosporidian infection in red junglefowl in China. Graphical Abstract

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“…Nucleic acids are vital components of living organisms, revealing a wealth of biological or pathological information. 1 Benefiting from its excellent sensitivity and specificity, the PCR method has become the gold standard for nucleic acid detection and has evolved as a versatile tool in disease diagnostics, 2 modern agriculture, 3 biological research, 4 and epidemic prevention such as COVID-19. 5,6 PCR-based nucleic acid detection often requires three key links: sample pretreatment, thermocycling amplification, and content characterization, as shown in Fig.…”

Section: Introductionmentioning

confidence: 99%

A sample-to-answer DNA detection microfluidic system integrating sample pretreatment and smartphone-readable gradient plasmonic photothermal continuous-flow PCR

Zhang

Zhao

Jiang

et al. 2022

Analyst

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Development and application of a novel multiplex PCR assay for the differentiation of four haemosporidian parasites in the chicken Gallus gallus domesticus

Cited by 10 publications

References 29 publications

Insights into the Biology of Leucocytozoon Species (Haemosporida, Leucocytozoidae): Why Is There Slow Research Progress on Agents of Leucocytozoonosis?

Insights into the Biology of Leucocytozoon Species (Haemosporida, Leucocytozoidae): Why Is There Slow Research Progress on Agents of Leucocytozoonosis?

First report of haemosporidia and associated risk factors in red junglefowl (Gallus gallus) in China

A sample-to-answer DNA detection microfluidic system integrating sample pretreatment and smartphone-readable gradient plasmonic photothermal continuous-flow PCR

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