Development and application of a novel in vivo overload model of the Achilles tendon in rat

Gains, Connor C; Giannapoulos, Antonios; Zamboulis, Danae Emilie; Lopez-Tremoleda, Jordi; Screen, Hazel R. C.

doi:10.1016/j.jbiomech.2023.111546

Journal of Biomechanics

2023

DOI: 10.1016/j.jbiomech.2023.111546

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Development and application of a novel in vivo overload model of the Achilles tendon in rat

Connor C Gains¹,

Antonios Giannapoulos²,

Danae Emilie Zamboulis³

et al.

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2024

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Collagen denaturation in post-run Achilles tendons and Achilles tendinopathy: In vivo mechanophysiology and magnetic resonance imaging

Fang,

Zhu,

Wei

et al. 2024

Sci. Adv.

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Achilles tendinopathy is often attributed to overuse, but its pathophysiology remains poorly understood. Disruption to the molecular structure of collagen is fundamental for the onset and progression of tendinopathy but has mostly been investigated in vitro. Here, we interrogated the in vivo molecular structure changes of collagen in rat Achilles tendons following treadmill running. Unexpectedly, the tendons’ collagen molecules were not mechanically unfolded by running but denatured through proteolysis during physiological post-run remodeling. We further revealed that running induces inflammatory gene expressions in Achilles tendons and that long-term running causes prolonged, elevated collagen degradation, leading to the accumulation of denatured collagen and tendinopathy development. For applications, we demonstrated magnetic resonance imaging of collagenase-induced Achilles tendon injury in vivo using a denatured collagen targeting contrast agent. Our findings may help close the knowledge gaps in the mechanobiology and pathogenesis of Achilles tendinopathy and initiate new strategies for its imaging-based diagnosis.

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Collagen denaturation in post-run Achilles tendons and Achilles tendinopathy: In vivo mechanophysiology and magnetic resonance imaging

Fang,

Zhu,

Wei

et al. 2024

Sci. Adv.

View full text Add to dashboard Cite

show abstract

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Development and application of a novel in vivo overload model of the Achilles tendon in rat

Cited by 1 publication

References 34 publications

Collagen denaturation in post-run Achilles tendons and Achilles tendinopathy: In vivo mechanophysiology and magnetic resonance imaging

Collagen denaturation in post-run Achilles tendons and Achilles tendinopathy: In vivo mechanophysiology and magnetic resonance imaging

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