Development and Application of an HPLC–UV Procedure to Determine Multiple Flavonoids and Phenolics in<i>Acanthopanax</i>Leaf Extracts

Zhang, Xiaodan; Zhou, Xinqi; Liu, Xiangqian; Li, Xiaolong; Whang, Wan Kyunn

doi:10.1093/chromsci/bmv189

Cited by 19 publications

(9 citation statements)

References 14 publications

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“…23 Many studies have revealed that rutin is good for human health, such as antibacterial, antiulcer, anti-inflammatory, antiarrhythmia, antitumor, protective and expanding blood vessel. 24,25 Up to now, lots of analytical methods have been applied for the determination of rutin, including chemiluminescence, 26,27 high-performance liquid chromatography (HPLC), 28,29 capillary electrophoresis spectrophotometry, 30,31 UV-Vis spectrophotometry 32 and electrochemical methods. [33][34][35][36] In this work, Cu-doped carbon quantum dots (Cu-CQDs) were prepared by a facile one-step pyrolytic synthesis using Na 2 [Cu(EDTA)] as precursor.…”

Section: Introductionmentioning

confidence: 99%

Fluorescent Sensors Based on Cu-Doped Carbon Quantum Dots for the Detection of Rutin

Wang¹,

Shang²,

Zheng³

et al. 2018

J. Braz. Chem. Soc.

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In this paper, Cu-doped carbon quantum dots (Cu-CQDs) were prepared by carrying out thermolysis of Na 2 [Cu(EDTA)] and hydroxylamine hydrochloride at 300 °C for 2 h. The asprepared Cu-CQDs were characterized by UV-Vis, Fourier transform infrared (FTIR) spectroscopy, transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD). The Cu-CQDs exhibited good fluorescence property, high stability, excitationdependent emission behavior and a quantum yield of 9.8%. More significantly, the synthesized Cu-CQDs were developed for sensing rutin (RT) based on the fluorescence quenching of the prepared Cu-CQDs. A good linear relationship between the fluorescence quenching of Cu-CQDs and different concentrations of RT was obtained in the range of 0.1-15 μg mL -1 with a correlation coefficient of 0.9959. The detection limit was as low as 0.05 μg mL -1 (signal-to-noise ratio, S/N = 3). The possible mechanism of fluorescence quenching was explored, which was proved to be inner filter effect. Importantly, the proposed method was successfully applied in the detection of RT tablet samples and with satisfactory results, showing the practical applications.Keywords: Cu-doped carbon quantum dots, rutin, Na 2 [Cu(EDTA)], fluorescence sensors Rutin tablet samples pretreatment 10 pieces of rutin tablets were ground to powder in a mortar, and 10 mg of the powder was dissolved in 25 mL of ethanol. Recovery experiments were performed by adding different concentrations of rutin to the treated samples. Results and Discussion Characterization of Cu-CQDsThe optical properties of the Cu-CQD were investigated by UV-Vis absorption and fluorescence emission spectra. The UV-Vis spectrum (Figure 2a) showed two shoulders at Fluorescent Sensors Based on Cu-Doped Carbon Quantum Dots for the Detection of Rutin J. Braz. Chem. Soc. 990

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Section: Introductionmentioning

confidence: 99%

Fluorescent Sensors Based on Cu-Doped Carbon Quantum Dots for the Detection of Rutin

Wang¹,

Shang²,

Zheng³

et al. 2018

J. Braz. Chem. Soc.

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“…Phytochemical studies have revealed that Acanthopanax spp. are rich in saponins , flavonoids , phenolics , terpenes , lignans , and other compounds . Various species including Acanthopanax senticosus, Acanthopanax gracilistylus , and Acanthopanax koreanum are particularly rich in saponins, which are considered to be the chemical markers of Acanthopanax .…”

Section: Introductionmentioning

confidence: 99%

Quantitative determination of 15 bioactive triterpenoid saponins in different parts of Acanthopanax henryi by HPLC with charged aerosol detection and confirmation by LC–ESI‐TOF‐MS

Zhang

Liu³

et al. 2016

J of Separation Science

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Triterpenoid saponins are difficult to analyze using high-performance liquid chromatography coupled to UV/vis spectrophotometry due to their lack of chromophores. This study describes the first analytical method for the determination of 15 triterpenoid saponins from the leaves, stems, root bark, and fruits of Acanthopanax henryi, using a high-performance liquid chromatography with charged aerosol detection coupled with electrospray ionization mass spectrometry method. The separation was carried out on a Kinetex XB-C18 column with an acetonitrile/water gradient as the mobile phase, followed by charged aerosol detection. The operating conditions of charged aerosol detection were set at 24 kPa for nitrogen pressure and 100 pA for the detection range. Liquid chromatography with electrospray ionization mass spectrometry is described for the identification of compounds in plant samples. The electrospray ionization mass spectrometry method involved the use of the [M + Na](+) and [M + NH4 ](+) ions for compounds 1-15 in the positive ion mode with an extracted ion chromatogram. The developed method was fully validated in terms of linearity, sensitivity, precision, repeatability, and recovery, then subsequently applied to evaluate the quality of A. henryi.

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“…19) Among Acanthopanax species, the leaves of Acanthopanax senticosus inhibit adipogenesis through down-regulation of lipoprotein lipase in 3T3-L1 cells. 21,22) Moreover, 50% ethanol extract is useful in treating non-alcoholic fatty liver disease in ob/ob mice and it reduces body weight gain in high-fat diet-induced obese mice. 23) This study focused on exploring the possibility that some plants belonging to the same Acanthopanax species might have effects on obesity or related disorders.…”

Section: Discussionmentioning

confidence: 99%

Anti-adipogenic effect of Glycoside St-E2 and Glycoside St-C1 isolated from the leaves of Acanthopanax henryi (Oliv.) Harms in 3T3-L1 cells

Han

et al. 2016

Bioscience, Biotechnology, and Biochemistry

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Acanthopanax henryi (Oliv.) Harms has been used in the treatment of arthritis, rheumatism, and abdominal pain. This study evaluated whether natural compounds isolated from the leaves of A. henryi (Oliv.) Harms could inhibit adipocyte differentiation by regulating transcriptional factors such as peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein α (C/EBPα). AMP-activated protein kinase (AMPK) activity was also evaluated. Among the several compounds isolated from the leaves of A. henryi (Oliv.) Harms, Glycoside St-C1 and Glycoside St-E2 significantly decreased lipid accumulation and the expressions of PPARγ and C/EBPα. Glycoside St-C1 and Glycoside St-E2 were found to activate AMPK when they regulated PPARγ and C/EBPα. Results confirmed that Glycoside St-C1 and Glycoside St-E2 isolated from the leaves of A. henryi (Oliv.) Harms can inhibit adipogenesis through the AMPK-PPARγ-C/EBPα mechanism. Thus, this study suggests that Glycoside St-C1 and Glycoside St-E2 have a therapeutic effect due to activation of the AMPKα.

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Development and Application of an HPLC–UV Procedure to Determine Multiple Flavonoids and Phenolics inAcanthopanaxLeaf Extracts

Cited by 19 publications

References 14 publications

Fluorescent Sensors Based on Cu-Doped Carbon Quantum Dots for the Detection of Rutin

Fluorescent Sensors Based on Cu-Doped Carbon Quantum Dots for the Detection of Rutin

Quantitative determination of 15 bioactive triterpenoid saponins in different parts of Acanthopanax henryi by HPLC with charged aerosol detection and confirmation by LC–ESI‐TOF‐MS

Anti-adipogenic effect of Glycoside St-E2 and Glycoside St-C1 isolated from the leaves of Acanthopanax henryi (Oliv.) Harms in 3T3-L1 cells

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