Development and Application of Sensitive Methods with Luminescence Detections for Determination of Biologically Active Compounds

Abstract

This review describes a comprehensive investigation in our laboratory focusing on development of sensitive analytical methods by utilizing luminescence detections, and leading to practical applications for quantification of biologically related compounds, especially drugs of abuse. The developed methods mainly used a high-performance liquid chromatographic separation technique with fluorescence and chemiluminescence detections. The main targets of our investigation are ultra-small amounts of biologically active compounds, especially centered on abused drugs such as stimulants, narcotics and anorectics in various matrices. Furthermore, drug-drug interaction of these compounds was also examined. The results obtained in our study might contribute to the prediction of and the protection of human health from the risks of abused drugs. The developed methods might be useful for pharmaceutical, clinical and forensic studies on drugs of abuse as well as other important biologically active compounds.

HPLC Analysis of Homocysteine and Related Compounds

“…Several eminent reviews have been published over the past decade regarding these detection tools, e.g., HPLC-UV/Vis detection [21], HPLC-FL detection [22], HPLC-luminescence detection [23], HPLC and/or capillary electrophoresis [2,3], electrochemical assays [24], and chromatographic methods in the study of autism [20], together with their application to the quantification of Hcy and/or related compounds and descriptions of the importance of measuring these compounds. In this chapter, we review HPLC methods and their applications in recent publications (from 2008 to 2017).…”

Analysis of Drugs of Abuse in Biological Specimens

“…Many reviews have focused on the drugs of abuse themselves, [5][6][7][8] specific biological specimens, such as hair, 9,10) the methodology, 11,12) or the analytical instruments. [2][3][4] In this review, on the basis of reports published in the last three years (2008-2010) describing the analyses of drugs of abuse in biological specimens, different perspectives, such as the need for and the background of drug analysis in biological specimens, and difficulty of drug testing in biological specimens, are introduced together with some actual examples.…”