Development and application of Whole-herd-Sampling, qPCR-based- Testing, and Precision-Removal methods to Eliminate ASFV in Four Large Swine Herds in China

Abstract

Since the first ASFV case was reported in China in 2018, the conventional depopulation method to control ASF has proved unwieldy because of its high production intensity and complex trade network. To provide an alternative to conventional stamping out methods, we developed a” Whole-herd-Sampling, qPCR-based-Testing, and Precision-Removal” method by sampling every whole herd sampling and qPCR tests to determine the status of ASFV in herds and using a precision removal of identified sows. By developing and applying these methods, we successfully controlled ASF and eliminated the virus from 4 large swine herds from 2019 to 2020. The time to negative herd (TTNH) was 19, 28, 14, and 1 day from farm 1 to 4, respectively. Retention rates of pigs from farm 1 to farm 4 were 69.7%, 65%, 99.4%, and 99.72%, respectively. We anticipated that this innovative method would be a good alternative to the conventional stamping out method and greatly facilitate the control and eradication of ASFV in China and worldwide.