Development and assessment of kerateine nanoparticles for use as a hemostatic agent

Luo, Tao; Hao, Shilei; Chen, Xiaoliang; Wang, Ju; Yang, Qian; Wang, Shaobin; Weng, Yulan; Wei, Huimin; Zhou, Jin; Wang, Bochu

doi:10.1016/j.msec.2016.03.007

Cited by 60 publications

(47 citation statements)

References 34 publications

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“…There is a handful of studies demonstrating that keratin extracted from human hair has hemostatic properties and increases animals survival in the acute tissue injury model. 29,30 Control wounds on 4th day postinjury, showed inflammatory infiltrate consisting predominantly of neutrophils, with some additional monocytes and lymphocytes. It is well known neutrophils are mainly responsible for wound debridement at the first stage of healing, however, a large body of evidence suggests that excess of neutrophils can negatively influence tissue repair, due to their lack of target specificity, which in turn can destroy the normal tissue.…”

Section: Discussionmentioning

confidence: 99%

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The role of allogenic keratin‐derived dressing in wound healing in a mouse model

Konop

Sulejczak

Czuwara

et al. 2017

Wound Repair Regeneration

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Keratin is an interesting protein needed for wound healing and tissue recovery. We have recently proposed a new, simple and inexpensive method to obtain fur and hair keratin-derived biomaterials suitable for medical application. The aim of the study was to evaluate the role of the fur keratin-derived protein (FKDP) dressing in the allogenic full-thickness surgical skin wound model. The data obtained using scanning electron microscopy showed that employed processed biomaterial had higher surface porosity compared with control raw material. From the MTS test, it was found keratin biomaterial is not only toxic to the NIH/3T3 cell line (p < 0.05), but also enhances cell proliferation compared with the control. In vivo studies have shown keratin dressings are tissue biocompatible, accelerate wound closure and epithelialization to the statistically significant differences on day 5 (p < 0.05) in comparison to control wounds. Histological examination revealed, that in FKDP-treated wounds the inflammatory response contained predominantly macrophages whilst their morphological untreated variants showed mixed cell infiltrates rich in neutrophils. Predominant macrophages based response creates more favorable environment for healing. In FKDP-dressed wounds the number of microhemorrhages was also significantly decreased (p < 0.05) as compared with undressed wounds. Applied keratin dressing favors reconstruction of a more regular skin structure and assures better cosmetic effect in terms of scar formation and appearance. In conclusion, fur keratin-derived protein dressings significantly accelerated wound healing in the mouse model. Further studies are needed to determine the molecular mechanisms involved in the multilayer wound healing process and to assess the possible use of these dressings for medical purposes.

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Section: Discussionmentioning

confidence: 99%

“…We also assume, that our keratin dressing possesses some hemostatic properties. There is a handful of studies demonstrating that keratin extracted from human hair has hemostatic properties and increases animals survival in the acute tissue injury model …”

Section: Discussionmentioning

confidence: 99%

The role of allogenic keratin‐derived dressing in wound healing in a mouse model

Konop

Sulejczak

Czuwara

et al. 2017

Wound Repair Regeneration

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“…Over the past decades, keratins have been extracted from human hair, feather, wool, horns, hooves and nails [1][2][3], which were fabricated into the films, hydrogels, dressing and scaffolds for biomedical applications in terms of haemostasis, nerve regeneration, wound healing and bone regeneration [4][5][6][7]. In particular, the haemostatic application of keratin proteins derived from human hair has been paid special attention [6,8].…”

Section: Introductionmentioning

confidence: 99%

“…In particular, the haemostatic application of keratin proteins derived from human hair has been paid special attention [6,8]. The hair fibre consists of 50 À 60% keratins [9], and the human hair keratin nanoparticles have been fabricated and used for haemostasis, the results showed that keratin nanoparticles can significantly reduce the blood loss and coagulation time in the liver puncture and tail amputation in rat models [5].…”

Section: Introductionmentioning

confidence: 99%

Recombinant human hair keratin proteins for halting bleeding

Guo

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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Keratins derived from human hair have been widely used for tissue engineering. However, some drawbacks relative to the traditional keratins extracts have been found: (a): difficultly controlling the amino acid composition; (b): batch to batch inconsistent quality; and (c): producing complex keratin and keratin-associated proteins (KAPs), which problems have made some studies concerning human hair keratins stagnant, especially in the mechanism studies related to hemostasis of keratins. Herein, a type-I human hair keratin of K37 and a type-II human hair keratin of K81 were heterologously expressed and firstly used for haemostatic application. SDS-PAGE analysis shows that the recombinant keratins had higher purity compared to the extracted keratins. The circular dichroism (CD) spectra of K37 and K81 suggested that the secondary structures were rich in α-helix. In addition, the recombinant keratin proteins could enhance fibrin colt formation at the site of injury and decrease the bleeding time and blood loss in liver puncture and femoral artery injury rat models. This study provides a new strategy for future works involving design and mechanism studies of keratin biomaterials.

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“…Previous reports have shown that the keratin protein have unique characteristics of bioactivity, biocompatibility, biodegradability, and natural abundance. In recent years, keratin has been applied in wound healing [3], nerve and bone regenerations, hemostasis and cell culture [4], water purification, textile finishing, and composite materials [5]. erefore, a keratin-based material is a very attractive construction.…”

Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of Keratin/Alginate Blend Microparticles

Srisuwan

Srihanam

2018

Advances in Materials Science and Engineering

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The water-in-oil (W/O) emulsification-diffusion method was used for construction of keratin (Ker), alginate (Alg), and Ker/Alg blend microparticles. The Ker, Alg, and Ker/Alg blend solutions were used as the water phase, while ethyl acetate was used as the oil phase. Firstly, different concentrations of Ker solution was used to find suitable content. 1.6% w/v Ker solution was blended with the same concentration of the Alg solution for further microparticle construction. Results from scanning electron microscope analysis show that the microparticles have different shapes: spherical, bowl-like, porous, and hollow, with several sizes depending on the blend ratio. FTIR and TG analyses indicated that the secondary structure and thermal stability of the microparticles were influenced by the Ker/Alg blend ratio. The interaction between functional groups of keratin and alginate was the main factor for both β-sheet structure and Td,max values of the microparticles. The results suggested that Ker/Alg blend microparticles might be applied in many fields by varying the Ker/Alg ratio.

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Development and assessment of kerateine nanoparticles for use as a hemostatic agent

Cited by 60 publications

References 34 publications

The role of allogenic keratin‐derived dressing in wound healing in a mouse model

The role of allogenic keratin‐derived dressing in wound healing in a mouse model

Recombinant human hair keratin proteins for halting bleeding

Preparation and Characterization of Keratin/Alginate Blend Microparticles

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