Development and cell death domain-containing asparagine-rich protein (DCD/NRP): an essential protein in plant development and stress responses

Camargos, Luiz Fernando de; Fraga, Otto Teixeira; Oliveira, Celio Cabral; Silva, José Cleydson Ferreira da; Fontes, Elizabeth P. B.; Reis, Pedro A. B.

doi:10.1007/s40626-018-0128-z

Cited by 16 publications

(12 citation statements)

References 66 publications

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“…TFs act as converging nodes that integrate stress signals and programmed cell death responses. NAC TFs have been frequently associated with stress and cell death responses in several plants, including tobacco, oat, rice, maize, sunflower, and soybean 4 , 44 , 60 – 66 . The comprehensive analysis of SAGs and other genes associated with the control of PCD-related mechanisms in crops offers a new insight over the cellular mechanisms of stress adaption and PCD, raising new possibilities for biotechnological intervention and development of modern agribusiness.…”

Section: Discussionmentioning

confidence: 99%

Contrasting roles of GmNAC065 and GmNAC085 in natural senescence, plant development, multiple stresses and cell death responses

Melo

Lourenço-Tessutti

Fraga

et al. 2021

Sci Rep

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NACs are plant-specific transcription factors involved in controlling plant development, stress responses, and senescence. As senescence-associated genes (SAGs), NACs integrate age- and stress-dependent pathways that converge to programmed cell death (PCD). In Arabidopsis, NAC-SAGs belong to well-characterized regulatory networks, poorly understood in soybean. Here, we interrogated the soybean genome and provided a comprehensive analysis of senescence-associated Glycine max (Gm) NACs. To functionally examine GmNAC-SAGs, we selected GmNAC065, a putative ortholog of Arabidopsis ANAC083/VNI2 SAG, and the cell death-promoting GmNAC085, an ANAC072 SAG putative ortholog, for analyses. Expression analysis of GmNAC065 and GmNAC085 in soybean demonstrated (i) these cell death-promoting GmNACs display contrasting expression changes during age- and stress-induced senescence; (ii) they are co-expressed with functionally different gene sets involved in stress and PCD, and (iii) are differentially induced by PCD inducers. Furthermore, we demonstrated GmNAC065 expression delays senescence in Arabidopsis, a phenotype associated with enhanced oxidative performance under multiple stresses, higher chlorophyll, carotenoid and sugar contents, and lower stress-induced PCD compared to wild-type. In contrast, GmNAC085 accelerated stress-induced senescence, causing enhanced chlorophyll loss, ROS accumulation and cell death, decreased antioxidative system expression and activity. Accordingly, GmNAC065 and GmNAC085 targeted functionally contrasting sets of downstream AtSAGs, further indicating that GmNAC85 and GmNAC065 regulators function inversely in developmental and environmental PCD.

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Section: Discussionmentioning

confidence: 99%

Contrasting roles of GmNAC065 and GmNAC085 in natural senescence, plant development, multiple stresses and cell death responses

Melo

Lourenço-Tessutti

Fraga

et al. 2021

Sci Rep

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“…The genes can be classified as those that control development and common versatile defense responses in plants, irrespective of the stress type and genes that are specifically expressed in the presence of toxic metals. The first group would include genes encoding (i) enzymes involved in antioxidant response pathways— the whole set of radical scavenging enzymes, including proteins of the ascorbate–glutathione cycle, as well as the enzymes of supportive redox systems (glyoxalase system, methionine sulfoxide reductases) [ 18 , 79 ], (ii) proteins involved in SSC production, (iii) enzymes crucial for PA and Mel biosynthesis pathways [ 143 , 144 ], (iv) heat shock proteins and other chaperones responsible for proper folding and (de)aggregation of proteins [ 145 ], (v) DNA repair enzymes [ 44 ], and (vi) proteins regulating developmental processes, such as stress-responsive asparagine rich protein (NRP) [ 146 ], signal transducing mitogen-activated kinases (MPK) or proteins involved in chromatin modifications [ 141 ]. As depicted in the subsections above and in Table 2 , the priming agents predominantly affect the antioxidant activity, and numerous studies confirmed that enhanced expression of genes encoding antioxidant enzymes, superoxide dismutase (SOD), peroxidases (POD), and catalase (CAT), enzymes of the ascorbate–glutathione cycle, occurs as a priming effect [ 44 , 75 , 88 ].…”

Section: Genes That Can Be Targeted To Induce Priming Effects For Metallic Stressmentioning

confidence: 99%

Priming Strategies for Benefiting Plant Performance under Toxic Trace Metal Exposure

Wiszniewska

2021

Plants

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Combating environmental stress related to the presence of toxic elements is one of the most important challenges in plant production. The majority of plant species suffer from developmental abnormalities caused by an exposure to toxic concentrations of metals and metalloids, mainly Al, As, Cd, Cu, Hg, Ni, Pb, and Zn. However, defense mechanisms are activated with diverse intensity and efficiency. Enhancement of defense potential can be achieved though exogenously applied treatments, resulting in a higher capability of surviving and developing under stress and become, at least temporarily, tolerant to stress factors. In this review, I present several already recognized as well as novel methods of the priming process called priming, resulting in the so-called “primed state” of the plant organism. Primed plants have a higher capability of surviving and developing under stress, and become, at least temporarily, tolerant to stress factors. In this review, several already recognized as well as novel methods of priming plants towards tolerance to metallic stress are discussed, with attention paid to similarities in priming mechanisms activated by the most versatile priming agents. This knowledge could contribute to the development of priming mixtures to counteract negative effects of multi-metallic and multi-abiotic stresses. Presentation of mechanisms is complemented with information on the genes regulated by priming towards metallic stress tolerance. Novel compounds and techniques that can be exploited in priming experiments are also summarized.

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“…Similarly, GmNAC030 belongs to a partially overlapped regulatory circuit integrating drought, ER, and biotic stresses with natural senescence in planta , and thus is considered a GmNAC-SAG . GmNAC030 interacts with GmNAC081 (TERN subfamily), which is involved in both dPCD and ePCD [ 43 , 56 , 57 ].…”

Section: Leaf Senescence-associated Gmnac Genes: Expression Profile and In Silico Analysesmentioning

confidence: 99%

Senescence-Associated Glycine max (Gm)NAC Genes: Integration of Natural and Stress-Induced Leaf Senescence

Fraga

Melo

Quadros

et al. 2021

IJMS

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Leaf senescence is a genetically regulated developmental process that can be triggered by a variety of internal and external signals, including hormones and environmental stimuli. Among the senescence-associated genes controlling leaf senescence, the transcriptional factors (TFs) comprise a functional class that is highly active at the onset and during the progression of leaf senescence. The plant-specific NAC (NAM, ATAF, and CUC) TFs are essential for controlling leaf senescence. Several members of Arabidopsis AtNAC-SAGs are well characterized as players in elucidated regulatory networks. However, only a few soybean members of this class display well-known functions; knowledge about their regulatory circuits is still rudimentary. Here, we describe the expression profile of soybean GmNAC-SAGs upregulated by natural senescence and their functional correlation with putative AtNAC-SAGs orthologs. The mechanisms and the regulatory gene networks underlying GmNAC081- and GmNAC030-positive regulation in leaf senescence are discussed. Furthermore, new insights into the role of GmNAC065 as a negative senescence regulator are presented, demonstrating extraordinary functional conservation with the Arabidopsis counterpart. Finally, we describe a regulatory circuit which integrates a stress-induced cell death program with developmental leaf senescence via the NRP-NAC-VPE signaling module.

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Development and cell death domain-containing asparagine-rich protein (DCD/NRP): an essential protein in plant development and stress responses

Cited by 16 publications

References 66 publications

Contrasting roles of GmNAC065 and GmNAC085 in natural senescence, plant development, multiple stresses and cell death responses

Contrasting roles of GmNAC065 and GmNAC085 in natural senescence, plant development, multiple stresses and cell death responses

Priming Strategies for Benefiting Plant Performance under Toxic Trace Metal Exposure

Senescence-Associated Glycine max (Gm)NAC Genes: Integration of Natural and Stress-Induced Leaf Senescence

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