Development and characterisation of a novel and rapid lung eosinophil influx model in the rat

Werner-Klein, Melanie; Göggel, Rolf; Westhof, Andreas; Erb, Klaus J.

doi:10.1016/j.pupt.2008.03.002

Cited by 9 publications

(8 citation statements)

References 38 publications

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“…2 and 3). These results are in accordance with previously reported data using sensitized BN rats [11,19,20]. Despite the widerange of anti-inflammatory effects that rank PDE4 inhibitors as attractive potential replacements for glucocorticoids, their efficacy is limited by emesis, the main side effect [21,22].…”

Section: Discussionsupporting

confidence: 93%

Anti-asthmatic effect of ASP3258, a novel phosphodiesterase 4 inhibitor

Kobayashi¹,

Kubo²,

Hirano³

et al. 2012

International Immunopharmacology

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Section: Discussionsupporting

confidence: 93%

Anti-asthmatic effect of ASP3258, a novel phosphodiesterase 4 inhibitor

Kobayashi¹,

Kubo²,

Hirano³

et al. 2012

International Immunopharmacology

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“…injection of 1mg ovalbumin (OVA, Sigma-Aldrich, St. Louis, MO, USA) emulsified with 40 mg Imject Alum (Pierce, Rockford, IL USA) in a total volume of 1ml. In order to elicit an airway allergic response, these rats were placed in a chamber made of plastic and challenged with an OVA aerosol (1% (w/v) in 0.9% NaCl) nebulized by an ultrasonic nebulizer (PARI BOY, PARI GmbH, Sternberg, Germany) twice a day for 1hour from days 6 to 14.10 rats in the negative control group were sham immunized with 0.9% NaCl and then challenged with an aerosol of 0.9% NaCl at the same frequency as the asthma group [31], [32]. HE staining of lung in asthma was carried out (Fig.…”

Section: Methodsmentioning

confidence: 99%

Is Asthma Related to Choroidal Neovascularization?

Sun

Huang

et al. 2012

PLoS ONE

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BackgroundAge-related degeneration(AMD) and asthma are both diseases that are related to the activation of the complement system. The association between AMD and asthma has been debated in previous studies. The authors investigated the relationship between AMD and asthma systemically.Principal FindingsThe epidemiological study showed that asthma was related to choroidal neovascularization(CNV) subtype(OR = 1.721, P = 0.023). However, the meta-analysis showed there was no association between AMD and asthma. In an animal model, we found more fluoresce in leakage of CNV lesions by FA analysis and more angiogenesis by histological analysis in rats with asthma. Western blot demonstrated an elevated level of C3α-chain, C3α’-chain and VEGF. After compstatin was intravitreally injected, CNV leakage decreased according to FA analysis, with the level of C3 and VEGF protein decreasing at the same time.SignificanceThis study first investigated the relationship between AMD and asthma systematically, and it was found that asthma could be a risk factor for the development of AMD. The study may provide a better understanding of the disease, which may advance the potential for screening asthma patients in clinical practice.

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“…Another explanation may be that neutrophils were identified using detection of different neutrophil antigens. In the rat studies, neutrophils were detected using the expression of His48 (Enns et al 2008, Tiidus 2001, 2005), which may be a different protein with a different function than the 7/4 protein (Rosas et al 2010, van Goor et al 1991, Werner-Klein et al 2008). Therefore, estrogen may increase 7/4 antigen expression and decrease His48 antigen expression.…”

Section: Discussionmentioning

confidence: 99%

“…Depending on the EC model, neutrophils are evident at the injured muscle site as early as 6 h and as late as 3 days after ECs (Koh et al 2003, Pizza et al 2002, 2005). Neutrophils are detected in injured muscle by immunohistochemistry using antibodies that recognize protein antigens, such as His48 (van Goor et al 1991, Werner-Klein et al 2008), Ly6G/C (Daley et al 2008, Hestdal et al 1991, Jutila et al 1994) and 7/4 (Hirsch and Gordon 1983). Although the 7/4 antigen has been determined to be derived from the Ly6B gene located on mouse chromosome 15 (Rosas et al 2010), the function of this and the other two antigens is unknown.…”

mentioning

confidence: 99%

Estrogen modulates 7/4 antigen distribution within eccentrically contracted injured skeletal muscle

Fulkerson

Nicholas

Schneider

2015

Biotechnic & Histochemistry

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Eccentric contractions are skeletal muscle stretches with concurrent active force production; these contractions commonly occur during dynamic sports activities and can cause acute muscle injury. Recovery from this injury depends in part on pro-inflammatory processes, such as neutrophil infiltration at the injured site, which is affected by estrogen. The estrogen effect has been examined broadly, but without distinguishing between major compartments within muscle in which neutrophil infiltration can occur. Therefore, we compared neutrophil antigen expression in two compartments of eccentrically contracted muscle of ovariectomized mice with or without estrogen. To quantify neutrophil antigen expression, serial cross sections of muscle were immunolabeled with antibodies that recognize 7/4 or Ly6C/G, and then quantified using computer-assisted image analysis. At 48 h post-injury, estrogen-positive (E+) mice had more 7/4-positive and Ly6C/G-positive myofibers, increased 7/4 area percentage, and more 7/4-positive cells in the connective tissue. In addition, E+ mice showed more 7/4-positive myofibers that were Ly6C/G-negative and more Ly6C/G-positive myofibers that were 7/4-negative. These data suggest that in injured muscle, estrogen increases 7/4 antigen in connective tissue and myofibers and is associated with more Ly6C/G-positive myofibers when the 7/4 antigen is absent from these myofibers.

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Development and characterisation of a novel and rapid lung eosinophil influx model in the rat

Cited by 9 publications

References 38 publications

Anti-asthmatic effect of ASP3258, a novel phosphodiesterase 4 inhibitor

Anti-asthmatic effect of ASP3258, a novel phosphodiesterase 4 inhibitor

Is Asthma Related to Choroidal Neovascularization?

Estrogen modulates 7/4 antigen distribution within eccentrically contracted injured skeletal muscle

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