Development and characterisation of a Brassica carinata inbred line incorporating genes for low glucosinolate content from B. juncea

Márquez-Lema, Angustias; Fernández‐Martínez, José M.; Pérez‐Vich, Begoña; Velasco, Leonardo

doi:10.1007/s10681-008-9678-5

Cited by 15 publications

(2 citation statements)

References 29 publications

(39 reference statements)

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“…These authors postulated that genotypes containing mainly sinigrin showed a larger flowering cycle. Sinigrin is the main glucosinolate in Ethiopian mustard seeds and leaves [25]. Although it is difficult to state with precision the degree of participation of the major cell constituents of leaves (protein, lipids, starch, cellulose, etc.)…”

Section: Resultsmentioning

confidence: 99%

Prediction of Agro-Morphological and Nutritional Traits in Ethiopian Mustard Leaves (Brassica Carinata A. Braun) by Visible-Near-Infrared Spectroscopy

Martínez-Valdivieso

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Río-Celestino

2018

Foods

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The particular characteristics of some of the Ethiopian mustard accessions available from seed banks could be used to increase the production and the diversity of products available to consumers and to improve their general quality. The objectives of this study were to determine the genetic variability for agro-morphological (days to first flowering: DFF and leaf pubescence: LP) and nutritional traits (total phenolic content: TPC) among accessions, and to evaluate the potential of near-infrared spectroscopy (NIRS) to predict these traits in Ethiopian mustard leaves. A great variation was found for the traits evaluated. The reference values were regressed against different spectral transformations by modified partial least-squares (MPLS) regression. The coefficients of determination in cross-validation (R2cv) shown by the equations for DFF, LP and TPC were 0.95, 0.63 and 0.99, respectively. The standard deviation to standard error of cross-validation ratio (RPD), were for these traits, as follows: DFF: 4.52, LP: 1.53 and, TPC: 24.50. These results show that the equations developed for DFF and TPC in Ethiopian mustard, can be predicted with sufficient accuracy for screening purposes and quality control, respectively. In addition, the LP equation can be used to identify those samples with “low”, “medium” and “high” groups. From the study of the mean and deviation standard spectra, and regression vectors of MPLS models it can be concluded that some major cell components, highly participated in modelling the equations for these traits.

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Section: Resultsmentioning

confidence: 99%

Prediction of Agro-Morphological and Nutritional Traits in Ethiopian Mustard Leaves (Brassica Carinata A. Braun) by Visible-Near-Infrared Spectroscopy

Martínez-Valdivieso

Font

Río-Celestino

2018

Foods

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“…Serendipitously, in the Brassica genus, several crop species have genomes in common and the ability to perform interspecific hybridizations allows for the transfer of useful adaptive traits between species for targeted crop improvement through conventional breeding (Katche et al 2019). Interspecific crosses with the compatible allotetraploid species B. napus and B. juncea have resulted in B. carinata lines with decreased glucosinolate content (Márquez-Lema et al 2008) and increased seed oil content (Sheikh et al 2009), as well as increased overall genetic diversity (Sheikh et al 2011). Similarly, C22:1 levels in B. carinata were significantly reduced through crosses to both B. napus (Fernandez-Escobar et al 1988) and B. juncea (Getinet et al 1994).…”

Section: Introductionmentioning

confidence: 99%

Development of B. carinata with super-high erucic acid content through interspecific hybridization

et al. 2021

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Key message Disomic alien chromosome addition Brassica carinata lines with super-high erucic acid content were developed through interspecific hybridization with B. juncea and characterized using molecular, cytological and biochemical techniques. Abstract Brassica carinata [A.] Braun (BBCC, 2n = 34) is a climate-resilient oilseed. Its seed oil is high in erucic acid (> 40%), rendering it well suited for the production of biofuel and other bio-based applications. To enhance the competitiveness of B. carinata with high erucic B. napus (HEAR), lines with super-high erucic acid content were developed through interspecific hybridization. To this end, a fad2B null allele from Brassica juncea (AABB, 2n = 36) was introgressed into B. carinata, resulting in a B. carinata fad2B mutant with erucic acid levels of over 50%. Subsequently, the FAE allele from B. rapa spp. yellow sarson (AA, 2n = 20) was transferred to the fad2B B. carinata line, yielding lines with erucic acid contents of up to 57.9%. Molecular analysis using the Brassica 90 K Illumina Infinium™ SNP genotyping array identified these lines as disomic alien chromosome addition lines, with two extra A08 chromosomes containing the BrFAE gene. The alien chromosomes from B. rapa were clearly distinguished by molecular cytogenetics in one of the addition lines. Analysis of microspore-derived offspring and hybrids from crosses with a CMS B. carinata line showed that the transfer rate of the A08 chromosome into male gametes was over 98%, resulting in almost completely stable transmission of an A08 chromosome copy into the progeny. The increase in erucic acid levels was accompanied by changes in the proportions of other fatty acids depending on the genetic changes that were introduced in the interspecific hybrids, providing valuable insights into erucic acid metabolism in Brassica.

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Brassica carinata – a new molecular farming platform for delivering bio‐industrial oil feedstocks: case studies of genetic modifications to improve very long‐chain fatty acid and oil content in seeds

Taylor

Falk

Palmer

et al. 2010

Biofuels Bioprod Bioref

114

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Crop development and species diversity are important aspects of the emerging global bioeconomy, as is maximizing crop value through total crop utilization. We advocate development of Brassica carinata as a biorefinery and bioindustrial oils platform using traditional and molecular breeding techniques and tools. We review genetic studies and breeding efforts to develop elite B. carinata germplasm, work involving development of transformation and regeneration protocols, target gene isolation, and transgene expression. Genetic modification strategies using a B. carinata breeding line as a delivery platform for very long‐chain fatty acid‐enhanced/modified oils are presented as case studies. The target oil products are erucic acid (22:1 Δ13), docosadienoic acid (22:2 Δ5, Δ13) and nervonic acid (24:1 Δ15); in addition transgenic efforts to enhance B. carinata seed oil content are discussed. The overall advantages and current limitations to utilizing this crop are delineated. Other anticipated biobased products from a B. carinata platform may include, but are not limited to, the production of biolubricants, biofuels and biopolymers from the oil, biopesticides, antioxidants, as well as plant gums, and vegetable protein‐based bioplastics and novel food and feed products. In summation, this collaborative B. carinata breeding/germplasm development/value‐added molecular modification effort will not only contribute to the development of renewable feedstocks for the emerging Canadian bioeconomy (biorefinery/bioproducts), but also promises to generate positive economic and environmental benefits. Published in 2010 by John Wiley & Sons, Ltd.

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Development and characterisation of a Brassica carinata inbred line incorporating genes for low glucosinolate content from B. juncea

Cited by 15 publications

References 29 publications

Prediction of Agro-Morphological and Nutritional Traits in Ethiopian Mustard Leaves (Brassica Carinata A. Braun) by Visible-Near-Infrared Spectroscopy

Prediction of Agro-Morphological and Nutritional Traits in Ethiopian Mustard Leaves (Brassica Carinata A. Braun) by Visible-Near-Infrared Spectroscopy

Development of B. carinata with super-high erucic acid content through interspecific hybridization

Brassica carinata – a new molecular farming platform for delivering bio‐industrial oil feedstocks: case studies of genetic modifications to improve very long‐chain fatty acid and oil content in seeds

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