SummaryThe study was conducted to formulate pasta with fish protein isolate (FPI) (0%, 2.5 %, 5.0%, 7.5% and 10.0%) and assessed its functional, cooking, textural, proximate, FTIR, and sensory attributes in comparison to control. Results showed that addition of FPI significantly influenced the functional properties. The protein (12.25–20.79%) and ash (0.40–3.21%) content increased significantly (p < 0.05), while cooking time, swelling index, and cooked weight, decreased. The supplementation also increases the firmness (0.181‐0.440kg) and toughness (0.680‐1.33 kg/sec) of pasta. FT‐IR spectra show the presence of the amide I, II, and III groups, with peaks at 1636 cm‐1, 1542 cm‐1 and 1228 cm‐1 with increased intensity as concentration increases. Pasta (uncooked and cooked) exhibits decrease in whiteness and increase in yellowness and redness with increased FPI levels. Pasta with 5% FPI was found acceptable by the panellists with an acceptable fish odour and highest overall acceptability scores.