Development and characterisation of structural changes in chromosome 3Hch from Hordeum chilense in common wheat and their use in physical mapping

Said, Mahmoud; Recio, Rocío; Cabrera, Adoración

doi:10.1007/s10681-012-0712-2

Cited by 16 publications

(33 citation statements)

References 48 publications

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“…This approach was subsequently applied to barley (Shi and Endo 1999) and rye (Friebe et al 2000). The chromosome 2C c induces frequent breakage in the centromeric region of barley chromosomes, with high rate of telocentric chromosomes and whole-arm translocations (Endo 2009 (Said et al 2012) of H. chilense. Alien introgression is an important strategy in both common (Khlestkina 2014) and durum wheats (Ceoloni et al 2014).…”

Section: Discussionmentioning

confidence: 99%

“…2C) monosomic for the gametocidal 2C were backcrossed with CS followed by five generations of selfing (Cifuentes et al 2005). The breeding procedure used to obtain these cytogenetic stocks was that described in (Said et al 2012).…”

Section: Plant Materialsmentioning

confidence: 99%

“…Root tips were prepared as described by Said et al (2012). Preparation of somatic metaphase chromosomes and FISH protocol were carried out as described by Cabrera et al (2002).…”

Section: Fluorescence In Situ Hybridization (Fish)mentioning

confidence: 99%

“…The availability of molecular markers capable of distinguishing between H. chilense chromatin in wheat background constitutes an important tool for selection. Barley expressed sequence tag (EST) have been successfully transferred to H. chilense (Hagras et al 2005a, b) and they have been used for physical mapping of chromosomes 4H ch (Said and Cabrera 2009), 1H ch (Cherif-Mouaki et al 2011) and 3H ch (Said et al 2012). In addition, the development of the conserved orthologous set (COS) (Quraishi et al 2009) signifies an additional source of markers potentially useful for H. chilense.…”

Section: Introductionmentioning

confidence: 99%

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Cytological and molecular characterization of wheat-Hordeum chilense chromosome 7Hch introgression lines

et al. 2014

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Chromosome 7H ch of Hordeum chilense carries the Phytoene synthase 1 (Psy1) gene encoding the first step in the carotenoid biosynthetic pathway. As such it can be used in the improvement of seed carotenoid content in wheat. Four introgressions of chromosome 7H ch into wheat have been characterized by in situ hybridization of labeled DNA probes and by several sets of DNA markers. Chromosome-specific SSR were used for the identification of wheat chromosomes. Besides 113 conserved orthologous set (COS) markers were tested for homoeologous group 7, of which 97 amplified in H. chilense and 32 were polymorphic between H. chilense and wheat, and 28 expressed sequence tag (EST) barley markers previously allocated to chromosome 7. A total of 60 markers (32 COS and 28 EST) were allocated to chromosome 7H ch with 28 assigned to 7H ch S and 22 to 7H ch L. A combination of in situ probing and marker genotyping have shown that among the four introgressions there was a substitution line 7H ch (7D), a ditelosomic addition line for the long arm of 7H ch and two homozygous centric translocations 7H ch SÁ2DS and 7H ch SÁ5AL. The Psy1 gene was localized on the short arm of 7H ch . The positions of markers from the international barley consortium map (IBSC2012) were determined and the comparative arm location between H. chilense and H. vulgare is discussed. The genetic stocks characterized here include new wheat-H. chilense recombinations useful for genetic studies and with a potential for breeding.

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Section: Discussionmentioning

confidence: 99%

Section: Plant Materialsmentioning

confidence: 99%

“…Root tips were prepared as described by Said et al (2012). Preparation of somatic metaphase chromosomes and FISH protocol were carried out as described by Cabrera et al (2002).…”

Section: Fluorescence In Situ Hybridization (Fish)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cytological and molecular characterization of wheat-Hordeum chilense chromosome 7Hch introgression lines

et al. 2014

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“…Root tips were prepared as described in Said et al (2012). Preparations were made as described by Cabrera et al (1999).…”

Section: Fluorescence In Situ Hybridization (Fish)mentioning

confidence: 99%

Molecular and cytogenetic characterization of a common wheat-Agropyron cristatum chromosome translocation conferring resistance to leaf rust

et al. 2014

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Crested wheatgrass (Agropyron cristatum L. Gaertn.) is a perennial species of economic importance as forage that also displays potentially valuable traits for wheat improvement trough intergeneric hybridization. In order to incorporate resistance genes from A. cristatum against wheat leaf rust (Puccinia triticina Erikss.) into common wheat (Triticum aestivum L.) a breeding programme was carried out by crossing and backcrossing the self-fertile amphiploid AABBDDPP (2n = 8x = 56) with T. aestivum (2n = 6x = 42; AABBDD). The AABBDDPP amphiploid was previously obtained by crossing tetraploid wheat (Triticum turgidum L. conv. durum Desf. 2n = 4x = 28; AABB) with a self-fertile allotetraploid (2n = 4x = 28; DDPP) between diploid wheat (Aegilops tauschii Coss.) and crested wheatgrass (A. cristatum). After three backcrosses a fertile stable line (named TH4) was obtained with 42 chromosomes. The fluorescence in situ hybridization and GISH analysis confirmed that TH4 carries a compensating robertsonian translocation involving the long arm of wheat chromosome 1B and the short arm of an unidentified A. cristatum chromosome. Specific molecular markers from A. cristatum also demonstrated the presence of chromatin from this species in the TH4 line. Macroscopic and microscopic observations indicated that the A. cristatum fragment that has been transferred to common wheat contributed a substantial level of partial resistance to leaf rust. The A. cristatum translocation line in bread wheat makes the disease resistance gene(s) from A. cristatum accessible for wheat breeding programmes.

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Sequence analysis of wheat subtelomeres reveals a high polymorphism among homoeologous chromosomes

Aguilar

Prieto

2020

The Plant Genome

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Bread wheat, Triticum aestivum L., is one of the most important crops in the world. Understanding its genome organization (allohexaploid; AABBDD; 2n = 6x = 42) is essential for geneticists and plant breeders. Particularly, the knowledge of how homologous chromosomes (equivalent chromosomes from the same genome) specifically recognize each other to pair at the beginning of meiosis, the cellular process to generate gametes in sexually reproducing organisms, is fundamental for plant breeding and has a big influence on the fertility of wheat plants. Initial homologous chromosome interactions contribute to specific recognition and pairing between homologues at the onset of meiosis. Understanding the molecular basis of these critical processes can help to develop genetic tools in a breeding context to promote interspecific chromosome associations in hybrids or interspecific genetic crosses to facilitate the transfer of desirable agronomic traits from related species into a crop like wheat. The terminal regions of chromosomes, which include telomeres and subtelomeres, participate in chromosome recognition and pairing. We present a detailed molecular analysis of subtelomeres of wheat chromosome arms 1AS, 4AS, 7AS, 7BS and 7DS. Results showed a high polymorphism in the subtelomeric region among homoeologues (equivalent chromosomes from related genomes) for all the features analyzed, including genes, transposable elements, repeats, GC content, predicted CpG islands, recombination hotspots and targeted sequence motifs for relevant DNA-binding proteins. These polymorphisms might be the molecular basis for the specificity of homologous recognition and pairing in initial chromosome interactions at the beginning of meiosis in wheat. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Development and characterisation of structural changes in chromosome 3Hch from Hordeum chilense in common wheat and their use in physical mapping

Cited by 16 publications

References 48 publications

Cytological and molecular characterization of wheat-Hordeum chilense chromosome 7Hch introgression lines

Cytological and molecular characterization of wheat-Hordeum chilense chromosome 7Hch introgression lines

Molecular and cytogenetic characterization of a common wheat-Agropyron cristatum chromosome translocation conferring resistance to leaf rust

Sequence analysis of wheat subtelomeres reveals a high polymorphism among homoeologous chromosomes

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