Mimosa bimucronata (Candolle) O. Kuntze, native to the American tropics, is the worst invasive tree species in China, where it is distributed in the southern coastal areas of Guangdong and Fujian provinces. It is characterized by rapid growth and waterlogging resistance, and was introduced in China for slope and bank protection. However, there is still a lack of effective molecular markers for its invasion management. In this study, we used a qubit dsDNA HS assay kit to extract DNA, and DNBSEQ-500 whole genome re-sequencing technology to obtain single nucleotide polymorphism (SNP) markers. In total, we developed and characterized 165 SNP markers. Observed (H O ) and expected heterozygosity (H E ) ranged from 0.0667 to 0.1000, and 0.1800 to 0.5000, respectively. The polymorphic information content (PIC) ranged from 0.1638 to 0.3750. These SNP markers will help to analyze the population genetic structure and assess the invasion risk for M. bimucronata.
Full TextMimosa bimucronata (Candolle) O. Kuntze, native to the American tropics, is the worst invasive tree species in China, and has been listed by China's State Council (https://www.gov.cn/) to be in the fourth batch of alien invasive species in China. M. bimucronata produces numerous propagules, leading to a wide propagation range, rapid growth, and strong reproductive ability (Zeng et al. 2013). It can therefore easily form a strong community in a short time and overcrowd local species, and has caused serious ecological harm in Guangdong, Fujian, and Hainan provinces. M. bimucronata can grow in a variety of soil conditions (Xu. 2014). It is heat, waterlogging, and drought-resistant with strong adaptability (Liu and Liu. 2011). It was rst introduced to China as a slope and bank revetment plant. It can also be used as rewood, and to study degraded habitats or green space restoration in tropical areas (Lan et al. 2019).Previous studies (e.g., Lan et al. 2019) have mostly focused on the morphological characteristics, distribution range, and ecological habits of M. bimucronata. So far, there is insu cient comprehensive research on the genetic variation of the main Chinese population of M. bimucronata, and limited information on its genome and effective molecular markers, which further hinders research on its management. Therefore, there is an urgent need to carry out genetic research on major M. bimucronata populations in China to better manage and protect surrounding natural resources and biodiversity. As a third-generation genetic marker (Liu et al. 2019; Qu et al. 2019), SNPs are the most convenient and rapid DNA markers in genetic research due to their wide genomic distribution and polymorphism (Gao et al. 2019;Li et al. 2018; Xu et al. 2021). SNP has the advantages of CO dominance, high polymorphism, strong representativeness, and wide distribution (Sun et al. 2021; Wang et al. 2022;). Therefore, DNBSEQ-500 whole-genome sequencing technology was used to develop and characterize SNPs to provide genetic markers for population genetic diversity and conservation gen...