Development and characterization of ceftriaxone-loaded P3HB-based microparticles for drug delivery

Murueva, Anastasiya V.; Shershneva, Anna M.; Abanina, Kseniya V.; Prudnikova, Svetlana V.; Shishatskaya, Ekaterina I.

doi:10.1080/07373937.2018.1487451

Cited by 19 publications

(12 citation statements)

References 68 publications

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“…The data obtained from in vitro CIN releasing behavior were fitted to various mathematical models, e.g., zero-order, first-order, Higuchi, Hixson-Crowell, Korsmeyer–Peppas, to determine the CIN release kinetics. The fitted model was subsequently selected based on a comparison of the relevant correlation coefficients [ 41 ].…”

Section: Methodsmentioning

confidence: 99%

Fabrication and Characterization of Cinnamaldehyde-Loaded Mesoporous Bioactive Glass Nanoparticles/PHBV-Based Microspheres for Preventing Bacterial Infection and Promoting Bone Tissue Regeneration

et al. 2021

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Polyhydroxybutyrate-co-hydroxyvalerate (PHBV) is considered a suitable polymer for drug delivery systems and bone tissue engineering due to its biocompatibility and biodegradability. However, the lack of bioactivity and antibacterial activity hinders its biomedical applications. In this study, mesoporous bioactive glass nanoparticles (MBGN) were incorporated into PHBV to enhance its bioactivity, while cinnamaldehyde (CIN) was loaded in MBGN to introduce antimicrobial activity. The blank (PHBV/MBGN) and the CIN-loaded microspheres (PHBV/MBGN/CIN5, PHBV/MBGN/CIN10, and PHBV/MBGN/CIN20) were fabricated by emulsion solvent extraction/evaporation method. The average particle size and zeta potential of all samples were investigated, as well as the morphology of all samples evaluated by scanning electron microscopy. PHBV/MBGN/CIN5, PHBV/MBGN/CIN10, and PHBV/MBGN/CIN20 significantly exhibited antibacterial activity against Staphylococcus aureus and Escherichia coli in the first 3 h, while CIN releasing behavior was observed up to 7 d. Human osteosarcoma cell (MG-63) proliferation and attachment were noticed after 24 h cell culture, demonstrating no adverse effects due to the presence of microspheres. Additionally, the rapid formation of hydroxyapatite on the composite microspheres after immersion in simulated body fluid (SBF) during 7 d revealed the bioactivity of the composite microspheres. Our findings indicate that this system represents an alternative model for an antibacterial biomaterial for potential applications in bone tissue engineering.

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Section: Methodsmentioning

confidence: 99%

Fabrication and Characterization of Cinnamaldehyde-Loaded Mesoporous Bioactive Glass Nanoparticles/PHBV-Based Microspheres for Preventing Bacterial Infection and Promoting Bone Tissue Regeneration

et al. 2021

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“…According to Murueva et al. (2019), this indicates no phenomenon of particle erosion. These authors also show that the release of the active compound, ceftriaxone, encapsulated by a spray dryer, also followed Higuchi's model.…”

Section: Resultsmentioning

confidence: 94%

Solid lipid microparticles loaded with ascorbic acid: Release kinetic profile during thermal stability

Carvalho

Oriani

Oliveira

et al. 2021

J. Food Process. Preserv.

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This work aimed to study the stability by thermal cyclization of solid lipid microparticles (SLMs) loaded with ascorbic acid (AA). It studies thermal cyclization stability, SLMs were submitted to storage in stress conditions at two alternating temperatures (30 and 45°C) for 48 hr each cycle. The particles were characterized by thermal behavior using DSC, polymorphism, AA retention levels, and AA retention kinetic modeling. The analyses were performed on days 0, 7, 15, 30, and 45 of storage. At the end of the 45th day, the SLMs presented good AA retention values, equivalent to 76.39%, 74.25%–83.07%, respectively, for F1, F2, and F3. The results indicate that all the SLMs had controlled release and followed the Higuchi kinetic model, and the melting peaks remained stable throughout the storage period. The polymorphic analysis revealed that the β' crystals type was predominant in all the formulations studied. Novelty impact statement For the application of solid lipid microparticles (SLMs), it is necessary to study their physical stability during storage. The use of temperature fluctuations (thermal cyclization) allows the study of the polymorphic, thermal, and retention behavior of the asset in the SLMs. The particles obtained in this work proved to be stable (controlled release by Higuchi model) when subjected to thermal stress during the 45 days of storage, with AA retention ranging from 74.25% to 83.07%, β' crystals, and invariable melting properties.

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“…Particle size distribution and f-potential measurements were carried out using a Zetasizer Nano-Zs particle analyzer (Malvern Instruments, Worcestershire, UK), as proposed by Murueva et al [29] and P erez et al [30] The particle size determination was performed on the mixed INS-CS feeding solution, i.e. before injecting INS-CS mixed solutions (pH 3) into the nanospray dryer.…”

Section: Dynamic Light Scattering (Dls) and F-potentialmentioning

confidence: 99%

High molecular weight chitosan based particles for insulin encapsulation obtained via nanospray technology

et al. 2020

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The objective of this work was to obtain Chitosan (CS) based particles for Insulin (INS) encapsulation, via nanospray drying of a feeding solution containing equal amounts of both components (0.1% w/v total solids content). The process was performed at pH 3 which is out of the range for electrostatic interactions to occur. The analysis involved the nanoparticles (NP) characterization in the solution before drying (pH 3) by dynamic light scattering (DLS) and after re-hydration at different pHs (3< pH < 11). The dried product was characterized by Fourier-transform spectroscopy (FTIR), scanning electron microscopy (SEM) and differential scanning calorimetry (DSC). FTIR allowed detecting the chemical groups involved in INS-CS interactions. The encapsulation efficiency of the glassy NP was 62.3 ± 0.32% as determined by HPLC. Upon powder re-hydration, NP of diameter <200 nm were obtained, with a minority of them exceeding the micron. The change in the shape and temperature of the main endothermic DSC peak and the higher T g value of the NP would confirm the increase in INS thermal stability when entrapped in a CS matrix. In terms of biological activity an in-vitro system was assayed. 3T3-L1 fibroblasts were exposed to INS and Insulin-Chitosan nanoparticles (INS-CS NP). Both treatments showed AKT phosphorylation, which is an indication of AKT activation. The activity of AKT plays an essential role in cell metabolism (lipid and glucose), growth, proliferation, polarity, among others. This activity is a measure of the upstream cell signals, i.e. INS's receptor activity. Phosphorylated AKT was detected during the assay time for INS-CS NP, showing remarkable differences respect to single INS. Nanodrying technology could be used to trap INS into CS matrix keeping the specific hormone functions and protecting it from the hostile conditions of the body.

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Development and characterization of ceftriaxone-loaded P3HB-based microparticles for drug delivery

Cited by 19 publications

References 68 publications

Fabrication and Characterization of Cinnamaldehyde-Loaded Mesoporous Bioactive Glass Nanoparticles/PHBV-Based Microspheres for Preventing Bacterial Infection and Promoting Bone Tissue Regeneration

Fabrication and Characterization of Cinnamaldehyde-Loaded Mesoporous Bioactive Glass Nanoparticles/PHBV-Based Microspheres for Preventing Bacterial Infection and Promoting Bone Tissue Regeneration

Solid lipid microparticles loaded with ascorbic acid: Release kinetic profile during thermal stability

High molecular weight chitosan based particles for insulin encapsulation obtained via nanospray technology

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