Development and characterization of eleven polymorphic microsatellite loci from South China field mouse (Apodemus draco)

Gu, Xiaodong; Liu, Shaoying; Wang, Yuezhao; Wu, Hua

doi:10.1007/s10592-009-9868-2

Cited by 8 publications

(8 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…flavicollis and A . draco : As7, As11, As20 and As34 [ 42 ], TNF(CA) [ 43 ], SFM2 [ 44 ], and SCFM2, SCFM6 and SCFM9 [ 45 ] ( S1 File ). PCR products were run with a GS-500 ROX size standard on an ABI 310 Genetic Analyzer (Applied Biosystems), and scored using Genemapper version 3.7 (Applied Biosystems).…”

Section: Methodsmentioning

confidence: 99%

Disentangle the Causes of the Road Barrier Effect in Small Mammals through Genetic Patterns

et al. 2016

View full text Add to dashboard Cite

Road barrier effect is among the foremost negative impacts of roads on wildlife. Knowledge of the factors responsible for the road barrier effect is crucial to understand and predict species’ responses to roads, and to improve mitigation measures in the context of management and conservation. We built a set of hypothesis aiming to infer the most probable cause of road barrier effect (traffic effect or road surface avoidance), while controlling for the potentially confounding effects road width, traffic volume and road age. The wood mouse Apodemus sylvaticus was used as a model species of small and forest-dwelling mammals, which are more likely to be affected by gaps in cover such as those resulting from road construction. We confront genetic patterns from opposite and same roadsides from samples of three highways and used computer simulations to infer migration rates between opposite roadsides. Genetic patterns from 302 samples (ca. 100 per highway) suggest that the highway barrier effect for wood mouse is due to road surface avoidance. However, from the simulations we estimated a migration rate of about 5% between opposite roadsides, indicating that some limited gene flow across highways does occur. To reduce highway impact on population genetic diversity and structure, possible mitigation measures could include retrofitting of culverts and underpasses to increase their attractiveness and facilitate their use by wood mice and other species, and setting aside roadside strips without vegetation removal to facilitate establishment and dispersal of small mammals.

show abstract

Section: Methodsmentioning

confidence: 99%

Disentangle the Causes of the Road Barrier Effect in Small Mammals through Genetic Patterns

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Hence it is an excellent model system for biogeographic studies [16] . Previous studies of Apodemus considered this region to be the Pleistocene refugium or the radiation center for the East Asian Apodemus species [1] , [8] , [17] , [18] . However, there has not been sufficient evidence from morphometric and molecular studies to describe the phylogenetic relationship between A. draco and A. ilex , especially the biogeography in the south of the Hengduan Mountains and the Yunnan-Guizhou Plateau.…”

Section: Introductionmentioning

confidence: 99%

Phylogeographic Study of Apodemus ilex (Rodentia: Muridae) in Southwest China

Liu

Chen

et al. 2012

PLoS ONE

Self Cite

View full text Add to dashboard Cite

BackgroundThe Mountains of southwest China have complex river systems and a profoundly complex topography and are among the most important biodiversity hotspots in the world. However, only a few studies have shed light on how the mountains and river valleys promote genetic diversity. Apodemus ilex is a fine model for investigating this subject.Methodology/Principal FindingsTo assess the genetic diversity and biogeographic patterns of Apodemus ilex, the complete cytochrome b gene sequences (1,140 bp) were determined from 203 samples of A. draco/ilex that were collected from southwest China. The results obtained suggested that A. ilex and A. draco are sistergroups and diverged from each other approximately 2.25 million years ago. A. ilex could be divided into Eastern and Western phylogroups, each containing two sub-groups and being widespread in different geographical regions of the southern Hengduan Mountains and the western Yunnan - Guizhou Plateau. The population expansions of A. ilex were roughly from 0.089 Mya to 0.023 Mya.ConclusionsOur result suggested that A. ilex is a valid species rather than synonym of A. draco. As a middle-high elevation inhabitant, the phylogenetic pattern of A. ilex was strongly related to the complex geographical structures in southwest China, particularly the existence of deep river valley systems, such as the Mekong and Salween rivers. Also, it appears that the evolutionary history of A. ilex, such as lineage divergences and population expansions were strongly affected by climate fluctuation in the Late Pleistocene.

show abstract

“…Microsatellite marker is an ideal tool to analyze comparative phylogeography of organisms because of their abundance, high polymorphism content, co-dominance, and bi-parentally inherited characteristics (Hewltt 2000). Therefore, on basis of the microsatellite development of the striped field mouse, South China field mouse and Chevrier's field mouse (Wu et al 2008(Wu et al , 2009Gu et al 2009), we report in this paper the isolation and characterization of microsatellite DNA markers in the Korean field mouse and large-eared field mouse.…”

mentioning

confidence: 99%

Microsatellite DNA markers for comparative phylogeography analysis in the Korean field mouse (Apodemus peninsulae) and large-eared field mouse (Apodemus latronum)

Zhang²

2009

Conservation Genet Resour

Self Cite

View full text Add to dashboard Cite

The sympatric Apodemus species are excellent models for studying comparative phylogeography of organisms. On basis of the microsatellite development of three Apodemus species, we describe in this paper the isolation and characterization of microsatellite DNA markers in the two Apodemus species (Apodemus peninsulae and Apodemus latronum) from genomic DNA-enriched libraries. Ten and eight polymorphic loci were found in A. peninsulae and A. latronum, respectively. These loci were highly polymorphic with numbers of alleles per locus ranging from 11-20 and 6-16, and expected heterozygosity from 0.891-0.947 and 0.714-0.950 for A. peninsulae and A. latronum, respectively. Seven and six loci followed Hardy-Weinberg expectations for A. peninsulae and A. latronum, respectively. No significant linkage association was found among all these loci. These polymorphic microsatellite loci will be useful in studying comparative phylogeography of the sympatric Apodemus species.

show abstract

Development and characterization of eleven polymorphic microsatellite loci from South China field mouse (Apodemus draco)

Cited by 8 publications

References 10 publications

Disentangle the Causes of the Road Barrier Effect in Small Mammals through Genetic Patterns

Disentangle the Causes of the Road Barrier Effect in Small Mammals through Genetic Patterns

Phylogeographic Study of Apodemus ilex (Rodentia: Muridae) in Southwest China

Microsatellite DNA markers for comparative phylogeography analysis in the Korean field mouse (Apodemus peninsulae) and large-eared field mouse (Apodemus latronum)

Contact Info

Product

Resources

About