Development and characterization of five rainbow trout pituitary single-cell clone lines capable of producing pituitary hormones

Chen, Maria J.; Chiou, Pinwen Peter; Liao, Yu-Hsian; Lin, Chun-Mean; Chen, Thomas T.

doi:10.1677/joe-09-0315

Cited by 15 publications

(7 citation statements)

References 29 publications

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“…This is likely due to the increased in concentration of dose which had the satisfactory action on the female's gonads. According to Chen et al [3], the synchronization of ovulation in fish after the application of pituitary dose is very important aspect. Egg weight, egg number and percentage egg weight were higher in 4 mg/kg dosage than the other dosages.…”

Section: Discussionmentioning

confidence: 99%

“…Demand for fish in both rural and urban areas is very high and continues to grow in Tanzania by FAO in 2007 [1]. The African catfish (Clarias gariepinus) has gained popularity in the aquaculture sector of Tanzania in the recent times [2,3]. Its demand has increased as food, control of over-population in tilapia ponds and as bait for the Nile perch fishery [4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

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Effects of Different Catfish Pituitary Gland Extract Dosages on Eggs and Hatchlings Quantity of African Catfish, Clarias gariepinus at a Constant Latency Period

Moshaand¹,

Mlingi²

2018

J Aquac Res Development

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An experiment was conducted to assess the effects of different dosages of catfish pituitary glands extract (CPGE) on eggs and hatchlings quantity of African catfish, Clarias gariepinus at a constant latency period. In this experiment, four dosages (2 mg/kg, 3 mg/kg, 4 mg/kg and 5 mg/kg of female) were used. Each dosage extracted from a male (300 g to 305 g) was injected to individual female (350 g to 355 g) at three replications and a total of 12 females were injected. The females were striped after 17 hour at 26°C to 28°C, eggs were counted and incubated. After for 24 hours of incubation at 26°C to 28°C, all hatchlings were counted and recorded. The results indicated that there were significant differences (P<0.05) among the dosages on egg and hatchlings quantity. Egg weight, egg number and percentage egg weight were significantly (P<0.05) higher in dosage three (4 mg/kg) compared to other treatments. Total hatchlings and percentage hatchlings produced from striped females were significantly (P<0.05) higher compared to other dosages. In conclusion, the experiment indicated that higher eggs and hatchlings quantity at a constant latency period are attained with 4 mg/kg of female. Therefore, it is recommended that for better induction of ovulation in African catfish, 4 mg/kg of female pituitary gland extract should be used as suggested by this study.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of Different Catfish Pituitary Gland Extract Dosages on Eggs and Hatchlings Quantity of African Catfish, Clarias gariepinus at a Constant Latency Period

Moshaand¹,

Mlingi²

2018

J Aquac Res Development

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“…2015b; Eckart et al ., 2018), five pituitary gland (Chen et al . 2010), three milt (Vo et al . 2015b), two each of intestine (Kawano et al .…”

Section: Scenario Of Global Cell Line Development and Usementioning

confidence: 99%

Comprehensive update on inventory of finfish cell lines developed during the last decade (2010–2020)

Thangaraj

Narendrakumar

Geetha

et al. 2021

Reviews in Aquaculture

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Cell culture has become an indispensable part of modern research and is considered the best alternatives for different biological research on whole animals as they mimic the host cellular genetic homogeneity and also reduces the variability of in vivo responses. Hence, there has been increasing development of cell lines by different research groups and companies globally. For this reason, to cover the dearth of knowledge of newly developed fish cell lines, we have made a comprehensive update on new fish cell lines established in the past decade (2010)(2011)(2012)(2013)(2014)(2015)(2016)(2017)(2018)(2019)(2020) to reflect the number of cell lines now available. This review focuses on the appraisal of the literature and of work done in finfish cell culture around the globe during the last decade and summarizes 280 newly established fish cell lines derived from 20 different tissue types of 44 fish families belonging to 21 different countries raising the total finfish cell lines to 783. The highest number of cell lines developed in the past decade was from the fish of the family Cyprinidae, which are most widely cultured in Asian countries. Also, the review discusses the need of new cell line development, different methods of cell line development and further enumerates the various applications and future prospects for these cell lines which we believe will serve as a referral document for researchers in the field.

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“…Pituitary tissue culture requires the pituitary from many individuals for experiment because the fish pituitary is very small. Thus, differences between individuals can lead to a number of variations within or between experiments ( Chen et al, 2010 ). Pituitary cell culture requires the process of separating and culturing pituitary cells, which is difficult to reproduce consistently, and recovery periods can affect specific gene expression ( Chen et al, 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Response of Pituitary Cells and Tissues to Neurokinin B and F in the Nile tilapia

Mun¹,

Oh²,

Kwon³

2022

Dev. Reprod.

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Neurokinin B (NKB) is a neuropeptide involved in the regulation of reproductive endocrine system of vertebrate animals, including fish. However, the pathway of NKB action in fish has not been clearly elucidated. In order to clarify the effect of NKB and NKF (neurokinin F) on gonadotropic hormone (GTH) gene expression in the pituitary, we studied the changes of LHβ and FSHβ gene expressions by using two different pituitary culture methods (whole pituitary culture or dispersed pituitary cell culture). Pituitaries were removed from mature female and male Nile tilapia. Changes of LHβ and FSHβ gene expressions were measured and compared after the treatment with NKB or NKF peptides at concentrations 0 to 1,000 nM. Expression of GTH genes in the whole pituitary cultures treated with NKB or NKF peptides did not show significant difference except in female at one concentration when treated with NKF. On the contrary, there were significant changes of GTH gene expressions in the dispersed pituitary cell cultures when treated with NKB and NKF peptides. These results suggest that dispersed pituitary cell culture is more relevant than whole pituitary culture in studying the function of pituitary, and that NKB and NKF could act directly on the pituitary to regulate the expression of GTH genes.

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Development and characterization of five rainbow trout pituitary single-cell clone lines capable of producing pituitary hormones

Cited by 15 publications

References 29 publications

Effects of Different Catfish Pituitary Gland Extract Dosages on Eggs and Hatchlings Quantity of African Catfish, Clarias gariepinus at a Constant Latency Period

Effects of Different Catfish Pituitary Gland Extract Dosages on Eggs and Hatchlings Quantity of African Catfish, Clarias gariepinus at a Constant Latency Period

Comprehensive update on inventory of finfish cell lines developed during the last decade (2010–2020)

Response of Pituitary Cells and Tissues to Neurokinin B and F in the Nile tilapia

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