Development and characterization of gelatin based nanoparticles for targeted delivery of zidovudine

Jadhav, Namdeo; Tone, Jadhav S; Irny, Preeti; Nadaf, Sameer J.

doi:10.4103/2230-973x.119213

Cited by 11 publications

(4 citation statements)

References 15 publications

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“…Stability of our prepared cationic Gel.NPs was improved at +17.6 Mv. compared to -23.1 Mv as mentioned in a previous study [ 36 ]. This stability might have played an important role in maintaining the stable electrostatic interaction between Gel.NPs and the negatively charged recombinant NS2 gene to form the intended NS2 /Gel.NPs conjugate, in addition to facilitate the penetration of the conjugate into the negatively charged cell membrane and nucleus of the target bacteria which is assumed to enhance the gene delivery process more than the gene alone [ 34 ].…”

Section: Discussionmentioning

confidence: 63%

Gelatin nanoparticles enhance delivery of hepatitis C virus recombinant NS2 gene

et al. 2017

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BackgroundDevelopment of an effective non-viral vaccine against hepatitis C virus infection is of a great importance. Gelatin nanoparticles (Gel.NPs) have an attention and promising approach as a viable carrier for delivery of vaccine, gene, drug and other biomolecules in the body.Aim of workThe present study aimed to develop stable Gel.NPs conjugated with nonstructural protein 2 (NS2) gene of Hepatitis C Virus genotype 4a (HCV4a) as a safe and an efficient vaccine delivery system.Methods and resultsGel.NPs were synthesized and characterized (size: 150±2 nm and zeta potential +17.6 mv). NS2 gene was successfully cloned and expressed into E. coli M15 using pQE-30 vector. Antigenicity of the recombinant NS2 protein was confirmed by Western blotting to verify the efficiency of NS2 as a possible vaccine. Then NS2 gene was conjugated to gelatin nanoparticles and a successful conjugation was confirmed by labeling and imaging using Confocal Laser Scanning Microscope (CLSM). Interestingly, the transformation of the conjugated NS2/Gel.NPs complex into E. coli DH5-α was 50% more efficient than transformation with the gene alone. In addition, conjugated NS2/Gel.NPs with ratio 1:100 (w/w) showed higher transformation efficiency into E. coli DH5-α than the other ratios (1:50 and 2:50).ConclusionGel.NPs effectively enhanced the gene delivery in bacterial cells without affecting the structure of NS2 gene and could be used as a safe, easy, rapid, cost-effective and non-viral vaccine delivery system for HCV.

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Section: Discussionmentioning

confidence: 63%

Gelatin nanoparticles enhance delivery of hepatitis C virus recombinant NS2 gene

et al. 2017

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“…The initial “burst effect” occurs due to free drug binding at the surface of the nanospheres, and this will help to achieve the minimum therapeutic level, followed by second-phase release of drug up to 12 hours (97.5%±2%). 56 The mechanism of drug transport/permeation through the nanospheres was considered by fitting the release profile to the five kinetic models (first-order, Higuchi, Korsmeyer–Peppas, Baker and Lonsdale, and Hixon and Crowell). The data were analyzed by fitting them to Sigma plot version 9, and the best-fit curve with an R 2 of 0.9838 was acknowledged for the Korsmeyer–Peppas model.…”

Section: Resultsmentioning

confidence: 99%

Nanoparticle formulation by Büchi B-90 Nano Spray Dryer for oral mucoadhesion

Harsha¹,

Al‐Dhubiab²,

Nair³

et al. 2015

DDDT

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Diabetes is considered one of the main threats to global public health in this era. It is increasing rapidly in every part of the world; the prevalence of the disease will grow to the point where 366 million people will be affected by 2030. The prevalence of diabetes mellitus (DM) in the Saudi population is high, and the majority of patients suffer from type 2 DM. Marketed oral antidiabetic drugs have indicated poor tolerability during chronic treatments, and this contributes to the moderately large proportion of type 2 DM patients that remain inadequately managed. Vildagliptin nanospheres were prepared with aminated gelatin using a spray-drying method; narrow particle-size distribution was seen at 445 nm. The angle of repose was found to be θ <33.5°. The nanospheres appeared to be spherical with a smooth surface. The drug content and percentage yield of the nanospheres were found to be 76.2%±4.6% and 83%±2%, respectively. The nanosphere-swell profile was found to be 165%±7%. The pure drug was 100% dissolved in 30 minutes, and the nanosphere formulation took 12 hours to dissolve (97.5%±2%), and followed a Korsmeyer–Peppas kinetic model with an R2 of 0.9838. The wash-off test of nanospheres found that they exhibited an excellent mucoadhesive property at 86.7% for 8 hours. The stability-study data showed no changes in the physicochemical properties of the nanospheres, and suggested that the nanospheres be stored below room temperature. The amount of vildagliptin retained was 1.6% within 3 hours, and in comparison with the gelatin vildagliptin nanoparticles formulation, the percentage that was retained was much higher (98.2% in 12 hours).

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“…After placing the samples directly on a pan, they underwent 24 scans in the spectral region of 600 to 4000 cm -1 . 23…”

Section: Fourier Transformation-infrared Spectroscopymentioning

confidence: 99%

Solubility Enhancement of Poorly Water-Soluble Drug Ritonavir using Polyvinylpyrrolidone and Chitosan-based Platform Technique

Nilima N,

Nagesh H

2023

IJDDT

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The antiviral medication ritonavir (RTV) belongs to the biopharmaceutics classification system (BCS) class II drug category and is known for its high permeability and poor solubility. Therefore, the prime objective of this research was to increase the rate of dissolution and improve the solubility of RTV using a polymer system. Solid dispersions consisting of binary polymer components i.e., chitosan (CH) and polyvinylpyrrolidone (PVP) were prepared using microwave microwave-assisted physical mixing technique and optimization of solid dispersion was performed with 32 factorial designs. Dispersions were analyzed for their physicochemical characteristics by the use of X-ray diffraction (XRD), differential scanning calorimetry (DSC), and fourier transform infrared spectroscopy (FTIR). The polymeric dispersions prepared were evaluated for the release of RTV throughout 1 h in 0.1N sodium chloride in a USP class II dissolving device. The phase solubility study demonstrated a considerable improvement in the saturation solubility of RTV when CH and PVP K30 were present either individually or in combination. Virtual interaction studies between the drug and polymers revealed physical interactions driven by van der Waals and hydrophobic forces between RTV and excipients. Carbonyl, amine, and hydroxyl groups, among others, were probably present in both RTV and CH, which allowed for these interactions to take place. There was no discernible interaction between the RTV and the polymers included in the FTIR research. The dispersion strategies have improved the dissolving rate, according to the in-vitro drug release investigation. A formulation including a polymeric dispersion of the optimal concentration of PVP and chitosan may efficiently boost the release of RTV, according to the in-vitro release profile.

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Development and characterization of gelatin based nanoparticles for targeted delivery of zidovudine

Cited by 11 publications

References 15 publications

Gelatin nanoparticles enhance delivery of hepatitis C virus recombinant NS2 gene

Gelatin nanoparticles enhance delivery of hepatitis C virus recombinant NS2 gene

Nanoparticle formulation by Büchi B-90 Nano Spray Dryer for oral mucoadhesion

Solubility Enhancement of Poorly Water-Soluble Drug Ritonavir using Polyvinylpyrrolidone and Chitosan-based Platform Technique

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Development and characterization of gelatin based nanoparticles for targeted delivery of zidovudine

Cited by 11 publications

References 15 publications

Gelatin nanoparticles enhance delivery of hepatitis C virus recombinant NS2 gene

Gelatin nanoparticles enhance delivery of hepatitis C virus recombinant NS2 gene

Nanoparticle formulation by B&uuml;chi B-90&nbsp;Nano Spray Dryer for oral mucoadhesion

Solubility Enhancement of Poorly Water-Soluble Drug Ritonavir using Polyvinylpyrrolidone and Chitosan-based Platform Technique

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Nanoparticle formulation by Büchi B-90 Nano Spray Dryer for oral mucoadhesion