Development and characterization of gemcitabine hydrochloride loaded lipid polymer hybrid nanoparticles (LPHNs) using central composite design

Yalcin, Tahir Emre; Ilbasmiş-Tamer, Sibel; Takka, Sevgi

doi:10.1016/j.ijpharm.2018.06.063

Cited by 48 publications

(21 citation statements)

References 35 publications

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“…It was found that after 1 month storage, droplet size slightly increased at 40 °C in both formulations (p<0.05). The mean droplet size of the F5C and F5GC increased to about 494 nm and 443 nm at 40°C, respectively, in 6 months possibly due to aggregation [24,25]. This also demonstrated the low stability of NEs at 40 °C as a result of coalescence and aggregation.…”

Section: Storage Stability Of Nesmentioning

confidence: 80%

Development and characterization of camphor-loaded ozonated olive oil nanoemulsions

Yalcin¹,

Takka²

2020

JRP

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The purpose of present study is to prepare and characterize camphor-loaded ozonated olive oil nanoemulsions (NEs). In this study, olive oils were ozonated with different times (up to 24 h) and their viscosities were examined. NEs were prepared by high-energy ultrasonication technique and characterized according to droplet size distribution, zeta potential, microscopic evaluation, and storage stability. The effect of oil phase/aqueous phase volume ratio, sonication time, and co-solvent (glycerol) on formulations were evaluated. 6 h ozonated olive oil was chosen for developing NEs due to its liquid form and viscosity (189 mPas). The obtained camphor-loaded NEs (with or without glycerol) had nearly 300 nm droplet size with negative zeta potential. Microscopic analysis revealed the spherical shape of droplets. The long-term stability tests showed that camphor-loaded NEs with glycerol were more stable than NEs without glycerol at 4 °C and 25 °C. According to the results, ozonated olive oil based NEs with glycerol might be promising nanosystems for topical delivery of camphor.

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Section: Storage Stability Of Nesmentioning

confidence: 80%

Development and characterization of camphor-loaded ozonated olive oil nanoemulsions

Yalcin¹,

Takka²

2020

JRP

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“…The effect of RGDV-gemcitabine on A549 cell monolayer was assayed with wound healing model 29 . In brief, in a six-well plate A549 cells were at 37 °C and in 5% CO 2 grown for 24 h to reach a confluence of 80–90%.…”

Section: Methodsmentioning

confidence: 99%

Exploring the action of RGDV-gemcitabine on tumor metastasis, tumor growth and possible action pathway

Zhang

et al. 2020

Sci Rep

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The coupling of Arg-Gly-Asp-Val (RGDV) and gemcitabine led to a hypothesis that the conjugate (RGDV-gemcitabine) could inhibit tumor metastasis. To confirm this hypothesis the activities of RGDV-gemcitabine inhibiting tumor metastasis in vitro and in vivo were presented for the first time. AFM (atomic force microscopy) imaged that RGDV-gemcitabine was able to adhere onto the surface of serum-starved A549 cells, to block the extending of the pseudopodia. Thereby RGDV-gemcitabine was able to inhibit the invasion, migration and adhesion of serum-starved A549 cells in vitro. On C57BL/6 mouse model RGDV-gemcitabine dose dependently inhibited the metastasis of planted tumor towards the lung and the minimal dose was 0.084 µmol/kg/3 days. The decrease of serum TNF-α (tumor necrosis factor), IL-8 (interleukin-8), MMP-2 (matrix metalloprotein-2) and MMP-9 (matrix metalloprotein-9) of the treated C57BL/6 mice was correlated with the action pathway of RGDV-gemcitabine inhibiting the metastasis of the planted tumor towards lung.

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“…It should allow the diffusion of dissolved drug molecules through membrane to evaluate the dissolution rate of respirable size drug particles. In this study, a dialysis membrane (MWCO = 12,400 Da) was selected as this is less resistive and commonly used in drug diffusion studies 42–44 . The thickness of the hydrated dialysis membrane is ∼60 μm which is comparable to the thickness of the ciliated cells in the upper airway region (tracheobronchial region) of the lung 5 .…”

Section: Methodsmentioning

confidence: 99%

A STELLA simulation model for in vitro dissolution testing of respirable size particles

Eedara

Tucker

Das

2019

Sci Rep

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In vitro dissolution testing is a useful quality control tool to discriminate the formulations and to approximate the in vivo drug release profiles. A dissolution apparatus has been custom-made for dissolution testing of dry powder formulations in a small volume of stationary medium (25 μL spread over 4.91 cm2 area i.e. ~50 μm thick). To understand the system and predict the key parameters which influence the dissolution of respirable size particles, a simulation model was constructed using STELLA modeling software. Using this model, the permeation (dissolution followed by diffusion through the membrane) of two anti-tubercular drugs of differing solubilities, moxifloxacin (17.68 ± 0.85 mg mL−1) and ethionamide (0.46 ± 0.02 mg mL−1), from the respirable size particles and their diffusion from a solution were simulated. The simulated permeation profiles of moxifloxacin from solution and respirable size particles were similar, indicating fast dissolution of the particles. However, the simulated permeation profile of ethionamide from respirable size particles showed slower permeation compared to the solution indicating the slow dissolution of the respirable size particles of ethionamide. The sensitivity analysis suggested that increased mucus volume and membrane thickness decreased the permeation of drug. While this model was useful in predicting and distinguishing the dissolution behaviours of respirable size moxifloxacin and ethionamide, further improvement could be made using appropriate initial parameter values obtained by experiments.

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Development and characterization of gemcitabine hydrochloride loaded lipid polymer hybrid nanoparticles (LPHNs) using central composite design

Cited by 48 publications

References 35 publications

Development and characterization of camphor-loaded ozonated olive oil nanoemulsions

Development and characterization of camphor-loaded ozonated olive oil nanoemulsions

Exploring the action of RGDV-gemcitabine on tumor metastasis, tumor growth and possible action pathway

A STELLA simulation model for in vitro dissolution testing of respirable size particles

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