Development and Characterization of Hemicellulose-Based Films for Antibacterial Wound-Dressing Application

Abstract

Hemicelluloses are biopolymers with versatile properties for biomedical applications. Herein, hemicellulose (arabinoxylan)-based antibacterial film dressings were prepared and characterized. Arabinoxylan was isolated from psyllium husk. Blank and gentamicin-loaded films were prepared by the solvent cast method using glycerol as the plasticizer. The appropriate composition of the films was obtained by varying the amounts of arabinoxylan, glycerol, and gentamicin. The films were found to be transparent, smooth, bubble-free, flexible, and easily peelable with 2% to 3% arabinoxylan. They had uniform thickness and swelled up to 60% of their initial size. The mechanical properties and water vapor transmission rate through the films were found to be suitable for wound-dressing application. Fourier transform infrared spectroscopy (FTIR) analysis confirmed drug–film compatibility. In an in vitro release study, more than 85% of the gentamicin was released from the films in 12 h. The antibacterial activities of the gentamicin-loaded films were found to be close to the standard gentamicin solution. The films were found to be cytocompatible in cell viability assay. These results suggested that hemicellulose-based films are promising materials for the dressing of infected wounds.

Antibiotic-Loaded Psyllium Husk Hemicellulose and Gelatin-Based Polymeric Films for Wound Dressing Application

“…The GM particles were homogeneously dispersed throughout the films, without a sign of clumping of particles to form large agglomerates or sedimentation, which further validated proper mixing of the film components. The results of SEM analysis of the films are in close agreement with a previous study where drug particles were uniformly distributed within AX films [14].…”

“…Since all the other factors were similar for all AX-GL films, the increase in the thickness of AXGL5 and AXGL5D can be attributed to the presence of more solids in the films. The thickness of the AX-GL films was higher than that of the previously reported AX films due to the addition of GL to the formulation of AX-GL films [14].…”

“…The TS of the AX-GL films is higher than that of our previously reported AX films, indicating that the addition of GL to the film formulations enhanced the TS of the films. Similarly, due to the addition of GL to the film formulations, the EAB of the AXGL4 films was lower than that of the previously reported AX film (containing 2.5% AX) [14]. Moreover, the tensile strength of all AX-GL films was higher than that of electrospun gelatin-arabinoxylan-ferulate fiber, as reported by Aduba Jr et al [21].…”

“…However, both films exhibited almost equal maximum antibiotic release after 24 h, indicating that the films hold the provided antibiotic for a long period of time at infected wounds. These results suggest that inclusion of GL in the film formulation prolongs the release time of the GM as compared to the previously reported AX films [14].…”

Starch-Capped Silver Nanoparticles Impregnated into Propylamine-Substituted PVA Films with Improved Antibacterial and Mechanical Properties for Wound-Bandage Applications

“…Any breach in the skin by wound causes invasion of microorganisms into the sensitive underneath tissues that lead to wound infection. The advanced research on wound dressing development suggests that the ideal wound dressing should provide a wet environment to the wound, absorb exudates from the wound, should be highly biocompatible, having good antimicrobial activity for quick tissue regeneration and mechanical features to remain intact [ 3 , 4 , 5 , 6 , 7 ].…”