Development and characterization of interspecific hybrids between Oryza sativa and O. latifolia by in situ hybridization

Yi, Chuandeng; Tang, Shuzhu; Zhou, Yong; Gong, Zhiyun; Gu, Minghong

doi:10.1007/s11434-008-0399-x

Cited by 7 publications

(6 citation statements)

References 15 publications

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“…10, a linear relationships being 1.09 nm/V and 2.42 nm/V respectively are obtained. These proportionality constants are in agreement with those reported in the literature 62,[64][65][66] unlike the one related to the barrier layer thickness. These results confirm that the EIS measurements have no influence on the porous oxide layer.…”

Section: Scsupporting

confidence: 92%

Electrochemical Impedance Investigation of Anodic Alumina Barrier Layer

Benfedda

Hamadou

Benbrahim

et al. 2012

J. Electrochem. Soc.

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In the present work, well ordered nanoporous anodic aluminum oxides (AAO) have been prepared on aluminum by a two step anodization process in 0.5 M oxalic acid at various potentials. We report the properties and semiconducting characteristics of the porous alumina barrier layers by electrochemical impedance spectroscopy analysis (EIS). EIS is considered to be a highly sensitive and non-destructive technique that allows determining barrier oxide layer characteristics. Aluminum oxide barrier is considered as a semiconductor which acts as a p-n heterojunction at anodizing voltages up to 20 V. The alumina barrier layer structure consists of a hole transport inner layer and an electron transport outer layer. Doping densities, flatband potential as well as space charge layer thickness are discussed in correlation with anodizing potential. Barrier layer thicknesses measurements obtained by EIS were compared with those obtained after EIS measurements by direct scanning electron microscopy observations.

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Section: Scsupporting

confidence: 92%

Electrochemical Impedance Investigation of Anodic Alumina Barrier Layer

Benfedda

Hamadou

Benbrahim

et al. 2012

J. Electrochem. Soc.

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“…Although hybridization between cultivated and distantly related wild species are very difficult (Fu et al 2007;Jena et al 2016), and many researchers have demonstrated that it is possible to overcome the difficulties of wide cross, such as O. alta crossing with O. sativa, and to transfer elite genes to cultivated rice by crossing combined with embryo rescue culture (Mao et al 1995;Yan et al 1996;Yi et al 2008;Zhang et al 2014). By rescuing hybrid embryos, in vitro F 1 plantlets were obtained in 2x × 2x combinations between O. sativa and O. officinalis (Fu et al 2011).…”

Section: Introductionmentioning

confidence: 99%

In vitro induction of auto-allotetraploid in a newly developed wild rice line from Oryza alta Swallen

Zhang

Shivute

Shahid

et al. 2019

Plant Cell Tiss Organ Cult

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Oryza alta Swallen is an important germplasm for rice resistance breeding; however, its CCDD genome (2n = 48) resulted in low crossability when the wild rice was crossed with O. sativa and restricted the success of transferring the desirable traits into cultivated rice. Induction of polyploidy is an efficient way for overcoming the low crossability among different species. A new O. alta line, Huaye 5, was developed by our group in 2016, which had high fertility (64.93%) and photoperiod-insensitive. Huaye 5 was used to induce auto-allotetraploidy using tissue culture in the present study. The tissue culture system was established by comparing five basic media (N6, B5, MS, NB and MB), two hormones (2,4-D and 6-BA) for induction and two differentiation media (MS and NB), and then induced auto-allotetraploid in the wild rice line by colchicine. The medium and hormone combinations of NB + 2,4-D (2.5 mg/L) + 6-BA (1.0 mg/L) produced the induction rate of 20%, and MS medium was found to be a suitable medium for callus induction with a differentiation rate of 10.15%, and the treatment of 600 mg/L colchicine for 24 h was the best protocol for inducing auto-allotetraploid. Subsequently, auto-allotetraploid plants (2n = 96) were obtained in the present study and their ploidy levels were detected by using flow cytometry, stomata size and chromosomes count methods. Many inclusions in the parenchyma cells surrounding vascular bundle were observed in auto-allotetraploid rice compared to the parent. We developed a new germplasm from O. alta, and established a protocol of in vitro induction of auto-allotetraploid, which can be used for crossing with autotetraploid rice. Key messageWe have successfully established a protocol for the in vitro induction of auto-allotetraploid in Oryza alta, which can be used to cross with autotetraploid or neo-tetraploid rice.Communicated by Manoj Prasad.

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“…The crossability between O. sativa (2n=2X=24, AA genome) and O. alta (2n=4X=48, CCDD genome) was 5.30% (Fu et al 2007). The crossability between O. sativa (2n=2X=24, AA genome) and O. latifolia (2n=4X=48, CCDD genome) was 4.89% (Yi et al 2008). In this study, the crossability between O. sativa (2n=2X=24, AA genome) and O. ridleyi (2n=4X=48, HHJJ genome) was 0.71% due to the more distant relationship, which is the first step for transferring and utilizing the favorable exogenous genes from O. ridleyi.…”

Section: Discussionmentioning

confidence: 99%

“…The pollinated spikelets were wrapped in Kraft paper bags and sprayed with a solution of 0.22 mmol L -1 gibberellin (GA 3 ) 3 d after pollination (Jena and Khush 1984). Hybrid embryo rescue was conducted as described previously (Yi et al 2008). Briefly, after 10 d of pollination, the immature hybrid embryos that had inflated ovaries were peeled in a super clean bench and sterilized with 70% ethanol and 0.5% sodium hypochlorite, followed by washing three times with sterile water.…”

Section: Plant Materials and Embryo Rescuementioning

confidence: 99%

Identification and cell wall analysis of interspecific hybrids between Oryza sativa and Oryza ridleyi

Wang

et al. 2017

Journal of Integrative Agriculture

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Oryza ridleyi is an allotetraploid wild species with the HHJJ genome, and O. sativa is a diploid cultivated rice that has the AA genome. Although the wide hybrid between the two species is difficult to obtain, we overcome this difficulty by young embryo rescue. An obvious heterosis was primarily found for the plant height, tillering ability, vegetative vigor, etc. However, the hybrid panicle and culm traits were found to resemble that of the wild rice parent, O. ridleyi, for the long awns, exoteric purple stigma, grain shattering, dispersed panicles, and culm mechanical strength. Genomic in situ hybridization (GISH) analysis was subsequently performed on the mitotic metaphase chromosome of the root tips, and we determined that the hybrid is an allotriploid with 36 chromosomes and its genomic constitution is AHJ. Chemical analyses conducted on the culm of O. sativa, O. ridleyi, and their interspecific hybrids showed that major changes occurred in the xylose, glucose, and arabinose concentrations, which are correlated with the specific hemicellulose polymer and cellulose components that are important in the primary cell walls of green plants. Meanwhile, the culm anatomical analyses indicated that additional large vascular bundles and an extra sclerenchyma cell layer were found in O. ridleyi. Additionally, further thickening of the secondary cell walls of the cortical fiber sclerenchyma cells and the phloem companion cells was discovered in O. ridleyi and in the interspecific hybrids. These results imply that there may be a potential link between culm mechanical strength and culm anatomical structure.

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Development and characterization of interspecific hybrids between Oryza sativa and O. latifolia by in situ hybridization

Cited by 7 publications

References 15 publications

Electrochemical Impedance Investigation of Anodic Alumina Barrier Layer

Electrochemical Impedance Investigation of Anodic Alumina Barrier Layer

In vitro induction of auto-allotetraploid in a newly developed wild rice line from Oryza alta Swallen

Identification and cell wall analysis of interspecific hybrids between Oryza sativa and Oryza ridleyi

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