Development and characterization of microsatellite markers for the Chinese endangered medicinal plant Tetrastigma hemsleyanum

Wang, Y.H.; Chen, N.; Zhang, Y.C.; Fu, Chengxin

doi:10.4238/2014.october.31.21

Cited by 5 publications

(3 citation statements)

References 12 publications

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“…Methodological details for nuclear microsatellite genotyping are described in detail in Wang et al . ().…”

Section: Methodsmentioning

confidence: 97%

“…All sampled individuals were genotyped at eight nSSR loci using primers (Tehe10, Tehe17, Tehe44, Tehe135, Tehe153, Tehe158, Tehe220, Tehe272) (GenBank accession numbers: KF557574-KF557583) developed specifically for T. hemsleyanum using a double-suppression PCR method (Lian et al, 2006). Methodological details for nuclear microsatellite genotyping are described in detail in Wang et al (2014).…”

Section: Plant Materials and Sampling Designmentioning

confidence: 99%

See 1 more Smart Citation

Molecular phylogeography and ecological niche modelling of a widespread herbaceous climber, Tetrastigma hemsleyanum (Vitaceae): insights into Plio–Pleistocene range dynamics of evergreen forest in subtropical China

et al. 2015

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SummaryWarm-temperate evergreen (WTE) forest represents the typical vegetation type of subtropical China, but how its component species responded to past environmental change remains largely unknown. Here, we reconstruct the evolutionary history of Tetrastigma hemsleyanum, an herbaceous climber restricted to the WTE forest.Twenty populations were genotyped using chloroplast DNA sequences and nuclear microsatellite loci to assess population structure and diversity, supplemented by phylogenetic dating, ancestral area reconstructions and ecological niche modeling (ENM) of the species distributions during the Last Glacial Maximum (LGM) and at present.Lineages in Southwest vs Central-South-East China diverged through climate/tectonicinduced vicariance of an ancestral southern range during the early Pliocene. Long-term stability in the Southwest contrasts with latitudinal range shifts in the Central-South-East region during the early-to-mid-Pleistocene. Genetic and ENM data strongly suggest refugial persistence in situ at the LGM.Pre-Quaternary environmental changes appear to have had a persistent influence on the population genetic structure of this subtropical WTE forest species. Our findings suggest relative demographic stability of this biome in China over the last glacial-interglacial cycle, in contrast with palaeobiome reconstructions showing that this forest biome retreated to areas of today's tropical South China during the LGM.

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“…Methodological details for nuclear microsatellite genotyping are described in detail in Wang et al . ().…”

Section: Methodsmentioning

confidence: 97%

Section: Plant Materials and Sampling Designmentioning

confidence: 99%

Molecular phylogeography and ecological niche modelling of a widespread herbaceous climber, Tetrastigma hemsleyanum (Vitaceae): insights into Plio–Pleistocene range dynamics of evergreen forest in subtropical China

et al. 2015

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“…Thus, T. hemsleyanum production hardly meets the increasing market demand [ 8 ]. In recent years, T. hemsleyanum has become an endangered and precious species in China due to its medicinal and economic value [ 9 ], boasting a market price of $58.25 per kilogram [ 1 , 10 ]. As such, the production and utilization issues associated with a long growth cycle and many ecological environment requirements need to be solved urgently.…”

Section: Introductionmentioning

confidence: 99%

Induction, Flavonoids Contents, and Bioactivities Analysis of Hairy Roots and True Roots of Tetrastigma hemsleyanum Diels et Gilg

Wang

et al. 2023

Molecules

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The flavonoids in Tetrastigma hemsleyanum Diels et Gilg (T. hemsleyanum) have high medicinal value. However, because of slow growth and harsh ecological environments, T. hemsleyanum is currently an endangered species. In light of this, we present a detailed hairy root induction procedure as a promising alternative to true roots with medicinal value. The percentage of explants induced by Agrobacterium rhizogenes (A. rhizogenes) to produce hairy roots out of the total number of explants infected (induction rate 1) was 95.83 ± 7.22%, and the proportion of hairy roots that contained Rol B fragments among all the hairy roots with or without Rol B fragments (positive rate) was 96.57 ± 1.72%. The transformation was further confirmed by the expression of the GUS protein. A high-productive hairy root line was screened for the comparative profiling of six flavonoids with true roots using high-performance liquid chromatography (HPLC). The contents of (+)-catechin, (−)-epicatechin, neochlorogenic acid, luteolin-6-C-glucoside, and orientin were 692.63 ± 127.24, 163.34 ± 31.86, 45.95 ± 3.46, 209.68 ± 6.03, and 56.82 ± 4.75 μg/g dry weight (DW) of 30-day-old hairy roots, respectively, which were higher than those of 3-year-old true roots. Hairy roots have stronger antioxidant activity than true roots. Overall, the hairy roots of T. hemsleyanum could serve as promising alternative sources for the production of flavonoids with medicinal uses.

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The possible future changes in potential suitable habitats of Tetrastigma hemsleyanum (Vitaceae) in China predicted by an ensemble model

Wang

Qian

FenYao

et al. 2022

Global Ecology and Conservation

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Development and characterization of microsatellite markers for the Chinese endangered medicinal plant Tetrastigma hemsleyanum

Cited by 5 publications

References 12 publications

Molecular phylogeography and ecological niche modelling of a widespread herbaceous climber, Tetrastigma hemsleyanum (Vitaceae): insights into Plio–Pleistocene range dynamics of evergreen forest in subtropical China

Molecular phylogeography and ecological niche modelling of a widespread herbaceous climber, Tetrastigma hemsleyanum (Vitaceae): insights into Plio–Pleistocene range dynamics of evergreen forest in subtropical China

Induction, Flavonoids Contents, and Bioactivities Analysis of Hairy Roots and True Roots of Tetrastigma hemsleyanum Diels et Gilg

The possible future changes in potential suitable habitats of Tetrastigma hemsleyanum (Vitaceae) in China predicted by an ensemble model

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