Development and characterization of microsatellite loci in the mistletoe <i>Psittacanthus schiedeanus</i> (Loranthaceae)

González, Clementina; Harvey, Nick; Ornelas, Juan Francisco

doi:10.3732/apps.1400099

Cited by 8 publications

(9 citation statements)

References 16 publications

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“…PCRs were carried out in a 5‐μl volume with the Multiplex PCR Kit (Qiagen) using two primer mixes following the protocols of González et al . (). PCR products were run on an Applied Biosystems 3730xl automated sequencer with a GeneScan‐600 LIZ Size Standard (Applied Biosystems, Foster City, CA, USA) and visualized using G eneious v.6.1.8 (Biomatters Auckland, New Zealand).…”

Section: Methodsmentioning

confidence: 97%

“…DNA was amplified by PCR using 10 nuclear microsatellite loci specifically designed and standardized for P. schiedeanus (sensu Kuijt, 2009), but which show some successful cross-species amplifications (Gonz alez et al, 2015). PCRs were carried out in a 5-ll volume with the Multiplex PCR Kit (Qiagen) using two primer mixes following the protocols of Gonz alez et al (2015).…”

Section: Molecular Datamentioning

confidence: 99%

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The influence of climatic niche preferences on the population genetic structure of a mistletoe species complex

et al. 2017

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The prevalent view on genetic structuring in parasitic plants is that host-race formation is caused by varying degrees of host specificity. However, the relative importance of ecological niche divergence and host specificity to population differentiation remains poorly understood. We evaluated the factors associated with population differentiation in mistletoes of the Psittacanthus schiedeanus complex (Loranthaceae) in Mexico. We used genetic data from chloroplast sequences and nuclear microsatellites to study population genetic structure and tested its association with host preferences and climatic niche variables. Pairwise genetic differentiation was associated with environmental and host preferences, independent of geography. However, environmental predictors appeared to be more important than host preferences to explain genetic structure, supporting the hypothesis that the occurrence of the parasite is largely determined by its own climatic niche and, to a lesser degree, by host specificity. Genetic structure is significant within this mistletoe species complex, but the processes associated with this structure appear to be more complex than previously thought. Although host specificity was not supported as the major determinant of population differentiation, we consider this to be part of a more comprehensive ecological model of mistletoe host-race formation that incorporates the effects of climatic niche evolution.

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Section: Methodsmentioning

confidence: 97%

Section: Molecular Datamentioning

confidence: 99%

The influence of climatic niche preferences on the population genetic structure of a mistletoe species complex

et al. 2017

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“…(). Genomic DNA (200 ng) was digested using RsaI and the fragments originated were ligated to a MluI adaptor and amplified by PCR (González et al., ). Fragments with microsatellite regions were selected using probes AG, AC, AAC, CCG, CTG, and AAT, and subsequently cloned.…”

Section: Methodsmentioning

confidence: 99%

“…The isolation of microsatellites was performed by Genetic Marker Services (Brighton, UK; www.geneticma rkerservices.com). An enriched genomic library was constructed for E. heros following the protocol described by Edwards et al (1996), with some modifications proposed by Gonz alez et al (2015) and Letelier et al (2015). Genomic DNA (200 ng) was digested using RsaI and the fragments originated were ligated to a MluI adaptor and amplified by PCR (Gonz alez et al, 2015).…”

Section: Development and Characterization Of Eight Microsatellite Locimentioning

confidence: 99%

“…An enriched genomic library was constructed for E. heros following the protocol described by Edwards et al (1996), with some modifications proposed by Gonz alez et al (2015) and Letelier et al (2015). Genomic DNA (200 ng) was digested using RsaI and the fragments originated were ligated to a MluI adaptor and amplified by PCR (Gonz alez et al, 2015). Fragments with microsatellite regions were selected using probes AG, AC, AAC, CCG, CTG, and AAT, and subsequently cloned.…”

Section: Development and Characterization Of Eight Microsatellite Locimentioning

confidence: 99%

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Structure and genetic variation among populations of Euschistus heros from different geographic regions in Brazil

Husch

Ferreira

Seraphim

et al. 2018

Entomologia Exp Applicata

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The Neotropical brown stink bug, Euschistus heros (Fabricius) (Hemiptera: Pentatomidae), is a major Brazilian soybean pest. Aiming to provide relevant information to implement pest and insecticide resistance management, new microsatellite loci were developed for E. heros and used in a study of genetic diversity and population structure. The population analysis was performed using eight microsatellite loci from 17 samples (n = 243 individuals) collected in the major soybean‐producing regions in Brazil (northeastern, midwestern, and southern regions). These microsatellite loci provided high genetic diversity values on the whole extension for the studied region (He = 0.895; total number of alleles = 400). Neotropical brown stink bug populations in general displayed low genetic structure levels among the samples (overall ΦST = 0.009). An exception was the sample from the northeastern region, which showed a significant genetic differentiation (pairwise ΦST = 0.031–0.063). Bayesian cluster analysis confirmed these results, did not show population subdivision, and indicated considerable levels of gene flow. Significant correlations between genetic differences and geographic distance were obtained. The lowest estimate of migration was found in the population from São Desidério, which was also the most distant from the remaining populations based on genetic distance. Some plausible hypotheses for the low genetic differentiation among these populations are the fast expansion of soybean production areas, the main food source of E. heros, polyvoltinism, and possible influence of anthropogenic dispersal. All these factors could have led to high population densities, a wide distribution that may contribute to reduced population differentiation, and increased genetic diversity.

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Characterization of microsatellite loci for three species of Tomoplagia (Diptera: Tephritidae) and absence of cross-species amplification

et al. 2020

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Tomoplagia (Diptera: Tephritidae) are endophagous herbivores on flowerheads of Asteraceae, with varying levels of host specialization. We isolated, characterized, and tested the heterologous amplification for sets of microsatellites of three species from this genus associated with native hosts in the Espinhaço mountain range, Brazil. We used two protocols to build microsatellite-enriched libraries and characterized 12 polymorphic loci for each fruit-fly species. The samples of T. grandis, a monophagous species, presented a lower number of alleles than the samples of the oligophagous species T. incompleta and T. bicolor. The average observed heterozygosity did not vary among species and the high values of Polymorphism Information Content suggest that the characterized microsatellite loci are quite informative. The primers developed for each Tomoplagia species failed to amplify the microsatellite loci for the other two species, which indicates a low conservation of the flanking nucleotide sequences in the three evaluated species. The sets of polymorphic microsatellites characterized for these species of Tomoplagia may be used in future ecological and evolutionary studies that aim to detect patterns of diversity on a fine scale.

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Development and characterization of microsatellite loci in the mistletoe Psittacanthus schiedeanus (Loranthaceae)

Cited by 8 publications

References 16 publications

The influence of climatic niche preferences on the population genetic structure of a mistletoe species complex

The influence of climatic niche preferences on the population genetic structure of a mistletoe species complex

Structure and genetic variation among populations of Euschistus heros from different geographic regions in Brazil

Characterization of microsatellite loci for three species of Tomoplagia (Diptera: Tephritidae) and absence of cross-species amplification

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