Development and characterization of microsatellite markers for the fruit fly, Bactrocera tau (Diptera: Tephritidae)

Wei, Yan; Liu, Li; Suosheng, Huang; Li, Chaoxu; Ma, Zhiying; Qin, Weiquan

doi:10.1007/s13355-015-0359-2

Cited by 8 publications

(6 citation statements)

References 11 publications

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“…We found the lowest H O value at locus Tin06 (0.05-T. incompleta) and the highest value at loci Tbi02 for T. bicolor and Tgr06 for T. grandis (H E = 0.88). The number of alleles found for Tomoplagia species is consistent with those found in some other species of Tephritidae, such as Anastrepha fraterculus (7-18 alleles per locus, mean = 12.9; Manni et al 2015) and Ceratitis capitata (8-22 alleles per locus, mean = 13.6; Bonizzoni et al 2000), but exceed those found for Anastrepha suspensa (2-12 alleles per locus, mean = 6.1; Fritz and Schable 2004) and Bactrocera tau (2-9 alleles per locus, mean = 4.5; Yan et al 2015). Yan et al (2015) also found a locus with low observed heterozygosity for Bactrocera tau (H O = 0.069), as we found in T. incompleta (locus Tin06).…”

Section: Discussionsupporting

confidence: 88%

Characterization of microsatellite loci for three species of Tomoplagia (Diptera: Tephritidae) and absence of cross-species amplification

et al. 2020

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Tomoplagia (Diptera: Tephritidae) are endophagous herbivores on flowerheads of Asteraceae, with varying levels of host specialization. We isolated, characterized, and tested the heterologous amplification for sets of microsatellites of three species from this genus associated with native hosts in the Espinhaço mountain range, Brazil. We used two protocols to build microsatellite-enriched libraries and characterized 12 polymorphic loci for each fruit-fly species. The samples of T. grandis, a monophagous species, presented a lower number of alleles than the samples of the oligophagous species T. incompleta and T. bicolor. The average observed heterozygosity did not vary among species and the high values of Polymorphism Information Content suggest that the characterized microsatellite loci are quite informative. The primers developed for each Tomoplagia species failed to amplify the microsatellite loci for the other two species, which indicates a low conservation of the flanking nucleotide sequences in the three evaluated species. The sets of polymorphic microsatellites characterized for these species of Tomoplagia may be used in future ecological and evolutionary studies that aim to detect patterns of diversity on a fine scale.

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Section: Discussionsupporting

confidence: 88%

Characterization of microsatellite loci for three species of Tomoplagia (Diptera: Tephritidae) and absence of cross-species amplification

et al. 2020

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“…However, neither the new (SUW) nor the old (e.g., WYS and WOL) populations are in HWE (Table 1). In fact, due to a heterozygote deficiency, many populations of expansive species are not found to indicate HWE, e.g., among Lepidoptera (Wei et al 2013), Coleoptera (Wu et al 2016) and Diptera (Yan et al 2015). In the case of spiders, HWE has usually been noted in stable-range populations (Ramirez & Chi 2004;Ramirez et al 2007Ramirez et al , 2013.…”

Section: Discussionmentioning

confidence: 99%

Population structure of the expansive wasp spider (Argiope bruennichi) at the edge of its range

Wawer

Rutkowski

Krehenwinkel

et al. 2017

Journal of Arachnology

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Abstract. The wasp spider Argiope bruennichi (Scopoli, 1772) is of Mediterranean-Pontian origin, but for decades it has been expanding northwards, including into the territory of Poland. Based on well-documented expansion records, we can distinguish ''old'' (south-eastern and south-western) and ''new'' populations (north-eastern), respectively, from the 1930s to the 2000s. In Poland, some populations of A. bruennichi were expected to be more genetically isolated from others, due to distance effects or differential times of arrival. We evaluated whether the oldest populations were in a state of HardyWeinberg equilibrium (HWE), and whether recently founded populations were in an expansion phase. Specimens of A. bruennichi (n ¼ 184) were collected at six localities in Poland and single sampling sites in Italy and Japan. Nine microsatellite loci were amplified although only five were useful in the final analyses. Based on the genotypes obtained, we estimated basic measures of genetic diversity and tested for deviation from HWE. The results showed a low level of polymorphism amongst the investigated markers, and accordingly, we found a low genetic diversity in populations. Only populations from Italy and Japan, and one population from Poland, were in HWE. The level of genetic differentiation among sampling sites from Poland was also very low. The high dispersal ability of the wasp spider appears to have facilitated high gene flow among populations. The peripheral and recently settled populations were characterized by the highest heterozygosity and the lowest inbreeding coefficient (F IS ) . The remaining Polish populations are therefore still in the expansion phase, as indicated by deviations from HWE.

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“…Microsatellite markers have been developed and used for many fruit fly species (Augustinos et al 2008;Bonizzoni et al 2001;Buahom et al 2013;Leal et al 2021;Lin et al 2020;Qin et al 2018;Wang et al 2021;Yan et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Development of microsatellite markers for the Japanese orange fly, Bactrocera tsuneonis (Diptera: Tephritidae)

et al. 2022

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To monitor and control the Japanese orange fly Bactrocera tsuneonis, it is important to determine their dispersal ability. Therefore, to determine the genetic structure of this species, we developed microsatellite markers at 17 loci for B. tsuneonis. A total of 80 adult females and males randomly collected from abandoned orchards in the Oita and Yamaguchi Prefectures, in the western part of Japan, were used for polymorphism analysis using the microsatellite markers. The average number of alleles was 2.65 for the 17 loci, in which two loci did not show polymorphism. The expected heterozygosity ranged from 0 to 0.663, although the observed heterozygosity was relatively low between 0-0.105. The null allele frequencies varied among populations. Moreover, no polymorphism was found in the three larval populations collected in the Oita Prefecture. These results suggested a high degree of inbreeding for this species. Significant genetic differentiation between females and males of the same population was not observed, although it was observed between the Oita and Yamaguchi populations.A bottleneck effect was detected in Yamaguchi but not in Oita, and this is congruent with the history of an expanding distribution in this species. These markers could be used for population discrimination and to expand the geographic history of this species, potentially contributing to the pest management of B. tsuneonis.

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Development and characterization of microsatellite markers for the fruit fly, Bactrocera tau (Diptera: Tephritidae)

Cited by 8 publications

References 11 publications

Characterization of microsatellite loci for three species of Tomoplagia (Diptera: Tephritidae) and absence of cross-species amplification

Characterization of microsatellite loci for three species of Tomoplagia (Diptera: Tephritidae) and absence of cross-species amplification

Population structure of the expansive wasp spider (Argiope bruennichi) at the edge of its range

Development of microsatellite markers for the Japanese orange fly, Bactrocera tsuneonis (Diptera: Tephritidae)

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