Development and characterization of monoclonal antibodies to subgroup A avian leukosis virus

Qiu, Yinsheng; Li, X.; Fu, Loning; Cui, Zhizhong; Li, W.; Wu, Zhonghu; Sun, Shuhan

doi:10.1111/vco.12036

Cited by 4 publications

(1 citation statement)

References 15 publications

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“…The 50% tissue culture infective dose (TCID 50 ) of ALV-A was determined using a limiting dilution assay in a 96-well plate covered with chicken embryo fibroblast (CEF) from 9-day-old chicken embryos, according to the Reed-Muench method. The positive cells were identified using an indirect immunofluorescence assay (IFA) mediated by a monoclonal antibody (MAb) specific for ALV-A 13,14 .…”

Section: Methodsmentioning

confidence: 99%

Comparison of Viremia, Cloacal Virus Shedding, Antibody Responses and Pathological Lesions in Adult Chickens, Quails, and Pigeons Infected with ALV-A

Zhang

et al. 2019

Sci Rep

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Subgroup A of the avian leukosis virus (ALV-A) can cause severe pathological lesions and death in infected chickens, and its reported hosts have increased recently. To assess the susceptibility of adult chickens, quails, and pigeons to ALV-A, three sets of 250-day-old birds were intraperitoneally inoculated with ALV-A. Viremia and cloacal virus shedding were dynamically detected using an immunofluorescence assay (IFA), ALV-P27 antigen ELISA or RT-PCR; pathological lesions were assessed using tissue sections; ALV-A in tissues was detected by IFA; and ALV-A antibody responses were detected using antibody ELISA kits and an immune diffusion test. The results indicated that persistent viremia occurred in 80% (8/10) of infected chickens, and transient viremia occurred in 17% (2/12) of infected quails, but no viremia occurred in infected pigeons. Cloacal virus shedding occurred intermittently in 80% (8/10) of infected chickens and in 8% (1/12) of infected quails but did not occur in infected pigeons. Severe inflammatory pathological lesions occurred in the visceral tissues of most infected chickens, and mild lesions occurred in a few of the infected quails, but no pathological lesions occurred in the infected pigeons. The ALV-A virus was detected in the visceral tissues of most infected chickens but not in the infected quails and pigeons. Obviously different ALV-A antibody responses occurred in the infected chickens, quails and pigeons. It can be concluded that adult chickens, quails and pigeons have dramatically different susceptibilities to ALV-A. This is the first report on artificial infection by ALV-A in different birds.

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Section: Methodsmentioning

confidence: 99%

Comparison of Viremia, Cloacal Virus Shedding, Antibody Responses and Pathological Lesions in Adult Chickens, Quails, and Pigeons Infected with ALV-A

Zhang

et al. 2019

Sci Rep

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Identification of a linear B-cell epitope on the avian leukosis virus P27 protein using monoclonal antibodies

Qin

Zhu

et al. 2016

Arch Virol

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Avian leukosis virus (ALV) is an avian oncogenic retrovirus that can induce various clinical tumors. The capsid protein P27 is the group-specific antigen of ALV and has many viral antigen sites that are easy to detect. In this study, we produced a monoclonal antibody (mAb), 3A9, that is specific for the P27 protein. A series of partially overlapping peptides were screened to define (181)PPSAR(185) as the minimal linear epitope recognized by mAb 3A9. The identified epitope could be recognized by chicken anti-ALV and mouse anti-ALV P27 sera. The epitope was highly conserved among a number of ALV-A, ALV-B and ALV-J strains. MAb 3A9 might be a valuable tool for the development of new immunodiagnostic approaches for ALV, and the defined linear epitope might help further our understanding of the antigenic structure of the P27 protein.

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Identification of a novel linear B-cell epitope in the p27 of Avian leukosis virus

et al. 2017

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Development and characterization of monoclonal antibodies to subgroup A avian leukosis virus

Cited by 4 publications

References 15 publications

Comparison of Viremia, Cloacal Virus Shedding, Antibody Responses and Pathological Lesions in Adult Chickens, Quails, and Pigeons Infected with ALV-A

Comparison of Viremia, Cloacal Virus Shedding, Antibody Responses and Pathological Lesions in Adult Chickens, Quails, and Pigeons Infected with ALV-A

Identification of a linear B-cell epitope on the avian leukosis virus P27 protein using monoclonal antibodies

Identification of a novel linear B-cell epitope in the p27 of Avian leukosis virus

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