Development and characterization of new nanoscaled ultrasound active lipid dispersions as contrast agents

Marxer, Elena Eva Julianne; Brüßler, Jana; Becker, Andreas; Schümmelfeder, J.; Schubert, Rolf; Nimsky, Christopher; Bakowsky, Udo

doi:10.1016/j.ejpb.2010.12.007

Cited by 56 publications

(28 citation statements)

References 40 publications

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“…In addition, AFM may lead to wider nanoparticles due to the tip broadening effect, which takes place when the tip is in contact with soft or sticky biomaterials (Luykx, Peters, Van Ruth, & Bouwmeester, 2008;Yang et al, 2007). However, other authors have published AFM images of nanoemulsions with good results (Mao et al, 2009;Marxer et al, 2011). Accordingly to our results, Preetz, Hauser, Hause, Kramer and Mäder (2010) have reported differences between droplet sizes of nanoemulsions and nanocapsules, since they found that the mean droplet size determined by DLS was 150 nm, whereas it ranged between 50 and 500 nm if they were observed by TEM or AFM.…”

Section: Tem and Afm Imagesmentioning

confidence: 94%

Effect of processing parameters on physicochemical characteristics of microfluidized lemongrass essential oil-alginate nanoemulsions

et al. 2013

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Section: Tem and Afm Imagesmentioning

confidence: 94%

Effect of processing parameters on physicochemical characteristics of microfluidized lemongrass essential oil-alginate nanoemulsions

et al. 2013

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“…Good contrastenhanced effects have been observed in a number of reported studies, and phospholipid-shell and gas-core NBs have shown optimal contrast-enhancing abilities. [16][17][18][19] However, studies in vivo have focused on the contrast enhancement abilities of these agents in normal organs or tumors, not on the potential of NBs for the passive targeting of tumors. As a result, the purpose of this work was to fabricate nanosized, phospholipidshelled NBs with high ultrasonic-imaging efficiency and of a sufficiently small size to pass through the pores of tumor vasculature and achieve passive tumor targeting.…”

Section: Discussionmentioning

confidence: 99%

“…Nanoscale ultrasound contrast agents with various shells (polymers or phospholipids) and cores (gas, liquid, or solid) have been fabricated and exhibit good contrast enhancement. Based on several in vitro [16][17][18][19] and in vivo 20 studies, phospholipid-shell and gas-core NBs have shown optimal contrast enhancement abilities. However, research on NBs is still in the initial stages.…”

Section: Introductionmentioning

confidence: 99%

Nanobubbles for enhanced ultrasound imaging of tumors

Yin¹,

Wang²,

Zheng³

et al. 2012

IJN

126

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Abstract:The fabrication and initial applications of nanobubbles (NBs) have shown promising results in recent years. A small particle size is a basic requirement for ultrasound contrastenhanced agents that penetrate tumor blood vessel pores to allow for targeted imaging and therapy. However, the nanoscale size of the particles used has the disadvantage of weakening the imaging ability of clinical diagnostic ultrasound. In this work, we fabricated a lipid NBs contrast-enhanced ultrasound agent and evaluated its passive targeting ability in vivo. The results showed that the NBs were small (436.8 ± 5.7 nm), and in vitro ultrasound imaging suggested that the ultrasonic imaging ability is comparable to that of microbubbles (MBs). In vivo experiments confirmed the ability of NBs to passively target tumor tissues. The NBs remained in the tumor area for a longer period because they exhibited enhanced permeability and retention. Direct evidence was obtained by direct observation of red fluorescence-dyed NBs in tumor tissue using confocal laser scanning microscopy. We have demonstrated the ability to fabricate NBs that can be used for the in vivo contrast-enhanced imaging of tumor tissue and that have potential for drug/gene delivery.

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“…UCAs have been reported as therapeutic agents for targeted or controlled drug/gene release. Marxer et al [54] developed a new kind of drug carriers with an average particle size of 200–300 nm based on different lipid formulations (DPPC/CH, DPPC/PEG40S, DSPC/PEG40S). Compared with the commercially available contrast agent SonoVue, the carriers exhibited adjustable properties such as small size, biocompatibility, good ultrasound reflectivity, high loading capacity, and long circulation (Figure 4(a)).…”

Section: Commonly Used Nanocarriers For Ultrasound-mediated Deliverymentioning

confidence: 99%

“…Compared with the commercially available contrast agent SonoVue, the nanoscaled ultrasound active lipid dispersions showed good ultrasound reflectivity. (b) Visualization of diameters by atomic force microscopy [54, 55]. …”

Section: Figurementioning

confidence: 99%

Ultrasound-Mediated Local Drug and Gene Delivery Using Nanocarriers

Zhou

Chen

Wáng

et al. 2014

BioMed Research International

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With the development of nanotechnology, nanocarriers have been increasingly used for curative drug/gene delivery. Various nanocarriers are being introduced and assessed, such as polymer nanoparticles, liposomes, and micelles. As a novel theranostic system, nanocarriers hold great promise for ultrasound molecular imaging, targeted drug/gene delivery, and therapy. Nanocarriers, with the properties of smaller particle size, and long circulation time, would be advantageous in diagnostic and therapeutic applications. Nanocarriers can pass through blood capillary walls and cell membrane walls to deliver drugs. The mechanisms of interaction between ultrasound and nanocarriers are not clearly understood, which may be related to cavitation, mechanical effects, thermal effects, and so forth. These effects may induce transient membrane permeabilization (sonoporation) on a single cell level, cell death, and disruption of tissue structure, ensuring noninvasive, targeted, and efficient drug/gene delivery and therapy. The system has been used in various tissues and organs (in vitro or in vivo), including tumor tissues, kidney, cardiac, skeletal muscle, and vascular smooth muscle. In this review, we explore the research progress and application of ultrasound-mediated local drug/gene delivery with nanocarriers.

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Development and characterization of new nanoscaled ultrasound active lipid dispersions as contrast agents

Cited by 56 publications

References 40 publications

Effect of processing parameters on physicochemical characteristics of microfluidized lemongrass essential oil-alginate nanoemulsions

Effect of processing parameters on physicochemical characteristics of microfluidized lemongrass essential oil-alginate nanoemulsions

Nanobubbles for enhanced ultrasound imaging of tumors

Ultrasound-Mediated Local Drug and Gene Delivery Using Nanocarriers

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