Development and characterization of novel EST‐SSR markers for <i>Speranskia tuberculata</i> (Euphorbiaceae)

Fu, Yi; Ju, Miao-Miao; Ma, Haiching; Xin, Peiyao; He, Chengzhong; Jia, Dianzeng; Tian, Bin

doi:10.3732/apps.1600067

Cited by 2 publications

(3 citation statements)

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“…Three chloroplast fragments, psbJ-petA , psbB-psbF , and trnL-trnF , and two species-specific low-copy nuclear genes ( 6146 and 38274 ) developed using transcriptome data ( Fu et al, 2016 ) following the procedure described by Ye et al (2017c) were used to detect potential genetic variation in S. tuberculata ( Supplementary Table 1 ). The PCRs were performed following the protocol of Ma et al (2019) , except for different annealing temperatures ( Supplementary Table 1 ), and the products were sequenced by Sangon Biotech (Shanghai, China).…”

Section: Methodsmentioning

confidence: 99%

“…Six of the 18 nSSR markers developed for Speranskia species ( Fu et al, 2016 ), which produced reproducible, scorable, and polymorphic products, were used to detect genetic variation in S. tuberculata ( Table 2 ). The six nSSRs were amplified ( Fu et al, 2016 ) and then genotyped using a 3730xl automated Genetic Analyzer (Applied Biosystems, Foster City, CA, United States). Allele sizes were determined using GENEMAPPER 3.7 (Applied Biosystems).…”

Section: Methodsmentioning

confidence: 99%

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Insights Into the Significance of the Chinense Loess Plateau for Preserving Biodiversity From the Phylogeography of Speranskia tuberculata (Euphorbiaceae)

et al. 2021

Front. Plant Sci.

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The significance of the Chinese Loess Plateau (CLP) in maintaining biodiversity for northern China has rarely been shown, as previous phylogeographic studies are mostly woody species and they have revealed that Quaternary refugia are mainly located in mountain regions. We selected a drought-enduring endemic herb, Speranskia tuberculata (Euphorbiaceae), to determine its glacial refugia and postglacial demographic history. To this end, we sampled 423 individuals from 38 populations covering its entire geographic distribution. Three chloroplast DNA (cpDNA) fragments, two low-copy nuclear genes, and six nuclear microsatellites (nSSRs) were used and supplemented with ecological niche modeling (ENM) to infer the phylogeographic history of this species. Populations with private haplotypes and high haplotype diversity of cpDNA are mainly located in the CLP or scattered around northeastern China and the coastal region. Spatial expansion, detected using a neutrality test and mismatch distribution, may have resulted in a widely distributed ancestral cpDNA haplotype, especially outside of the CLP. For nuclear DNA, private haplotypes are also distributed mainly in the CLP. In nSSRs, STRUCTURE clustering identified two genetic clusters, which are distributed in the west (western cluster) and east (eastern cluster), respectively. Many populations belonged, with little to no admixture, to the western cluster while (hardly) pure populations of the eastern cluster were barely found. Genetic differentiation is significantly correlated with geographic distance, although genetic diversity is uniformly distributed. ENM suggests that the distribution of S. tuberculata has recently expanded northwards from the southern CLP, whereas it has experienced habitat loss in the south. Thus, S. tuberculata populations probably survived the last glacial maximum (LGM) in the southern CLP and experienced post-glacial expansion. Wind-dispersed pollen could bring the majority of genotypes to the front during spatial expansion, resulting in uniformly distributed genetic diversity. Based on evidence from molecular data and vegetation and climate changes since the LGM, we conclude that drought-enduring species, especially herbaceous species, are likely to have persisted in the CLP during the LGM and to have experienced expansion to other regions in northern China.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Insights Into the Significance of the Chinense Loess Plateau for Preserving Biodiversity From the Phylogeography of Speranskia tuberculata (Euphorbiaceae)

et al. 2021

Front. Plant Sci.

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“…is a typical example. It is a perennial herbaceous plant that is endemic to Northeast China and grows on grassy slopes, meadows, or thickets, usually in dry places, 300-1900 m in height [2,3]. Speranskia tuberculata is a drought-resistant plant.…”

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Biologically Active Copmounds in the Exrtacts of Speranskia Tuberculata (Bunge) Baill and Their Effect on the Viability of Cancer Cells of Five Different Lines

Sun,

Derkach

2024

Фарм. час.

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The aim of the work. Identifying the most considerable bioactive compounds in Speranskia tuberculata extracts and studying their effect on the viability of cancer cells of five lines. Materials and Methods. The herb Speranskia tuberculata extract was prepared based on ethyl acetate and petroleum ether in the 12.5-75 μg/ml concentration range. The presence of compounds in the extracts was investigated by the tandem high-performance liquid chromatography-mass spectroscopy (HPLC/MS) method. The MTT cytotoxicity test was used to evaluate the effectiveness of extracts on five commercially available cell lines: A549 (human lung adenocarcinoma), HEPG2 (human hepatocellular carcinoma of the liver), A375 (human malignant melanoma), HELA (human pancreatic carcinoma), RAW264.7 (macrophage-like cell line from mice transformed with Abelson's leukaemia virus). Results and Discussion. The peaks of about 300 compounds were identified in the mass spectra. Fifty-six compounds with the largest peaks were investigated from the point of view of manifestations of anticancer, antitumour, antioxidant and anti-inflammatory activity known from the literature. Only 35 of the 56 peaks have a documented effect on the listed diseases, another 15 compounds are active on other diseases, and 6 showed no activity. Of the 35 compounds belonging to 10 chemical classes, alkaloids, lipids, and polyphenols dominate (20 out of 35). Chemical reactions for phytochemical screening of chemical classes found in extracts are presented. In MTT tests, the viability during treatment of 4 out of 5 cell lines significantly decreases with increasing concentration of extracts. Only RAW264.7 cells were stable - their viability did not fall below 75-85%. Ethyl acetate extracts were the most effective. They maximally reduce viability to 18%, and the IC50 of this extract for all cell lines varied between 49-53 μg/ml. For petroleum ether, the IC50 of three lines was 65-74 μg/ml (for A375, the approximate value reached 114 μg/ml). Conclusions. The peaks of about 300 compounds found in extracts of the herb Speranskia tuberculata were identified by the HPLC/MS. Among the 56 most intense peaks, 35 compounds, according to the literature, have anticancer, antitumour, antioxidant and anti-inflammatory effects. MTT tests showed that the studied extracts significantly reduced the viability of cancer cells in 4 out of 5 lines. IC50 for ethyl acetate extracts is 49-53 μg/ml, and for petroleum ether - 65-74 μg/ml.

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Development and characterization of novel EST‐SSR markers for Speranskia tuberculata (Euphorbiaceae)

Cited by 2 publications

References 9 publications

Insights Into the Significance of the Chinense Loess Plateau for Preserving Biodiversity From the Phylogeography of Speranskia tuberculata (Euphorbiaceae)

Insights Into the Significance of the Chinense Loess Plateau for Preserving Biodiversity From the Phylogeography of Speranskia tuberculata (Euphorbiaceae)

Biologically Active Copmounds in the Exrtacts of Speranskia Tuberculata (Bunge) Baill and Their Effect on the Viability of Cancer Cells of Five Different Lines

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